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Article

Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling

1
Department of Microbiology, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, Egypt
2
Center for Drug Research and Development, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, Egypt
3
Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt
4
Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt
5
Department of Microbiology & Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt
6
Virology Department, Egyptian Center for Research and Regenerative Medicine (ECRRM), Cairo 11517, Egypt
7
Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt
8
Independent Researcher, 69126 Heidelberg, Germany
9
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt
10
Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia 61111, Egypt
11
Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
12
Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62512, Egypt
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Antibiotics 2020, 9(9), 629; https://doi.org/10.3390/antibiotics9090629
Received: 20 August 2020 / Revised: 5 September 2020 / Accepted: 8 September 2020 / Published: 22 September 2020
(This article belongs to the Special Issue Novel Antibiotics from Actinomycetes)
In the present study, we investigated the actinomycetes associated with the Red Sea-derived soft coral Sarcophyton glaucum in terms of biological and chemical diversity. Three strains were cultivated and identified to be members of genera Micromonospora, Streptomyces, and Nocardiopsis; out of them, Micromonospora sp. UR17 was putatively characterized as a new species. In order to explore the chemical diversity of these actinobacteria as far as possible, they were subjected to a series of fermentation experiments under altering conditions, that is, solid and liquid fermentation along with co-fermentation with a mycolic acid-containing strain, namely Nocardia sp. UR23. Each treatment was found to affect these actinomycetes differently in terms of biological activity (i.e., antitrypanosomal activity) and chemical profiles evidenced by LC-HRES-MS-based metabolomics and multivariate analysis. Thereafter, orthogonal projections to latent structures discriminant analysis (OPLS-DA) suggested a number of metabolites to be associated with the antitrypanosomal activity of the active extracts. The subsequent in silico screenings (neural networking-based and docking-based) further supported the OPLS-DA results and prioritized desferrioxamine B (3), bafilomycin D (10), and bafilomycin A1 (11) as possible antitrypanosomal agents. Our approach in this study can be applied as a primary step in the exploration of bioactive natural products, particularly those from actinomycetes. View Full-Text
Keywords: actinomycetes; co-fermentation; Micromonospora; metabolomics; docking; antitrypanosomal; OSMAC actinomycetes; co-fermentation; Micromonospora; metabolomics; docking; antitrypanosomal; OSMAC
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MDPI and ACS Style

Gamaleldin, N.M.; Bakeer, W.; Sayed, A.M.; Shamikh, Y.I.; El-Gendy, A.O.; Hassan, H.M.; Horn, H.; Abdelmohsen, U.R.; Hozzein, W.N. Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling. Antibiotics 2020, 9, 629. https://doi.org/10.3390/antibiotics9090629

AMA Style

Gamaleldin NM, Bakeer W, Sayed AM, Shamikh YI, El-Gendy AO, Hassan HM, Horn H, Abdelmohsen UR, Hozzein WN. Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling. Antibiotics. 2020; 9(9):629. https://doi.org/10.3390/antibiotics9090629

Chicago/Turabian Style

Gamaleldin, Noha M., Walid Bakeer, Ahmed M. Sayed, Yara I. Shamikh, Ahmed O. El-Gendy, Hossam M. Hassan, Hannes Horn, Usama R. Abdelmohsen, and Wael N. Hozzein 2020. "Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling" Antibiotics 9, no. 9: 629. https://doi.org/10.3390/antibiotics9090629

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