Bacteriostatic and Bactericidal Effect of Tigecycline on Leptospira spp.
Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy
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Antibiotics 2020, 9(8), 467; https://doi.org/10.3390/antibiotics9080467
Received: 30 June 2020
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Revised: 28 July 2020
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Accepted: 30 July 2020
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Published: 30 July 2020
(This article belongs to the Special Issue Global Antimicrobial Resistance: What Are the Current Emerging Threats?)
Tigecycline is a relatively new antimicrobial, belonging to glycylcyclines with antimicrobial activity against a large spectrum of bacteria. Very few data are available on its effect on Leptospira spp., which consist in a bacteriostatic mechanism. The aim of this investigation was to evaluate the bacteriostatic and bactericidal effect of tigecycline on reference Leptospira strains belonging to 16 serovars. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined through the microdilutions method, and tetracycline was used as the control. Results showed that tigecycline had higher MIC and MBC values than tetracycline. Obtained MIC values were between 4 and 32 µg/mL, while MBC values between 16 and >128 µg/mL. Patoc (MIC: 4 µg/mL; MBC: 16 µg/mL) resulted in the most susceptible serovar, while the most resistant were Bataviae (MIC: 32 µg/mL; MBC: 64 µg/mL), Bratislava (MIC: 8 µg/mL; MBC 128 µg/mL), and Tarassovi (MIC: 8 µg/mL; MBC: >128 µg/mL). This is the first investigation focused on the effect of tigecycline against Leptospira spp. reference strains. Since tigecycline is used as a treatment for bacteremia and urinary tract disease, and these symptoms could be linked to Leptospira infection, the possibility of using this antibiotic as a treatment for leptospirosis should be evaluated. Further studies are needed to explore the possibility to use tigecycline for in vivo application against Leptospira.
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Keywords:
Leptospira; minimum inhibitory concentration (MIC); microdilutions method; tigecycline; antibiotic susceptibility; leptospirosis
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MDPI and ACS Style
Bertelloni, F.; Cilia, G.; Fratini, F. Bacteriostatic and Bactericidal Effect of Tigecycline on Leptospira spp. Antibiotics 2020, 9, 467. https://doi.org/10.3390/antibiotics9080467
AMA Style
Bertelloni F, Cilia G, Fratini F. Bacteriostatic and Bactericidal Effect of Tigecycline on Leptospira spp. Antibiotics. 2020; 9(8):467. https://doi.org/10.3390/antibiotics9080467
Chicago/Turabian StyleBertelloni, Fabrizio, Giovanni Cilia, and Filippo Fratini. 2020. "Bacteriostatic and Bactericidal Effect of Tigecycline on Leptospira spp." Antibiotics 9, no. 8: 467. https://doi.org/10.3390/antibiotics9080467
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