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Article

Clonal Structure, Virulence Factor-encoding Genes and Antibiotic Resistance of Escherichia coli, Causing Urinary Tract Infections and Other Extraintestinal Infections in Humans in Spain and France during 2016

1
Laboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, Spain
2
Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), 15706 Santiago de Compostela, Spain
3
Service de Microbiologie, Hôpital Beaujon, AP-HP, 92110 Clichy, France
4
Université de Paris Diderot, IAME, UMR 1137, INSERM, F-75018 Paris, France
5
Unidade de Microbioloxía, Hospital Universitario Lucus Augusti (HULA), 27003 Lugo, Spain
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(4), 161; https://doi.org/10.3390/antibiotics9040161
Received: 27 February 2020 / Revised: 1 April 2020 / Accepted: 2 April 2020 / Published: 4 April 2020
(This article belongs to the Special Issue Pathogenic Escherichia coli: Infections and Therapies)
Escherichia coli is the main pathogen responsible for extraintestinal infections. A total of 196 clinical E. coli consecutively isolated during 2016 in Spain (100 from Lucus Augusti hospital in Lugo) and France (96 from Beaujon hospital in Clichy) were characterized. Phylogroups, clonotypes, sequence types (STs), O:H serotypes, virulence factor (VF)-encoding genes and antibiotic resistance were determined. Approximately 10% of the infections were caused by ST131 isolates in both hospitals and approximately 60% of these infections were caused by isolates belonging to only 10 STs (ST10, ST12, ST58, ST69, ST73, ST88, ST95, ST127, ST131, ST141). ST88 isolates were frequent, especially in Spain, while ST141 isolates significantly predominated in France. The 23 ST131 isolates displayed four clonotypes: CH40-30, CH40-41, CH40-22 and CH40-298. Only 13 (6.6%) isolates were carriers of extended-spectrum beta-lactamase (ESBL) enzymes. However, 37.2% of the isolates were multidrug-resistant (MDR). Approximately 40% of the MDR isolates belonged to only four of the dominant clones (B2-CH40-30-ST131, B2-CH40-41-ST131, C-CH4-39-ST88 and D-CH35-27-ST69). Among the remaining MDR isolates, two isolates belonged to B2-CH14-64-ST1193, i.e., the new global emergent MDR clone. Moreover, a hybrid extraintestinal pathogenic E.coli (ExPEC)/enteroaggregative isolate belonging to the A-CH11-54-ST10 clone was identified. View Full-Text
Keywords: E. coli; clonal structure; ST88; ST141; ST131; ST1193; virulence genes; resistance E. coli; clonal structure; ST88; ST141; ST131; ST1193; virulence genes; resistance
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MDPI and ACS Style

Flament-Simon, S.-C.; Nicolas-Chanoine, M.-H.; García, V.; Duprilot, M.; Mayer, N.; Alonso, M.P.; García-Meniño, I.; Blanco, J.E.; Blanco, M.; Blanco, J. Clonal Structure, Virulence Factor-encoding Genes and Antibiotic Resistance of Escherichia coli, Causing Urinary Tract Infections and Other Extraintestinal Infections in Humans in Spain and France during 2016. Antibiotics 2020, 9, 161. https://doi.org/10.3390/antibiotics9040161

AMA Style

Flament-Simon S-C, Nicolas-Chanoine M-H, García V, Duprilot M, Mayer N, Alonso MP, García-Meniño I, Blanco JE, Blanco M, Blanco J. Clonal Structure, Virulence Factor-encoding Genes and Antibiotic Resistance of Escherichia coli, Causing Urinary Tract Infections and Other Extraintestinal Infections in Humans in Spain and France during 2016. Antibiotics. 2020; 9(4):161. https://doi.org/10.3390/antibiotics9040161

Chicago/Turabian Style

Flament-Simon, Saskia-Camille, Marie-Hélène Nicolas-Chanoine, Vanesa García, Marion Duprilot, Noémie Mayer, María P. Alonso, Isidro García-Meniño, Jesús E. Blanco, Miguel Blanco, and Jorge Blanco. 2020. "Clonal Structure, Virulence Factor-encoding Genes and Antibiotic Resistance of Escherichia coli, Causing Urinary Tract Infections and Other Extraintestinal Infections in Humans in Spain and France during 2016" Antibiotics 9, no. 4: 161. https://doi.org/10.3390/antibiotics9040161

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