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Antibiotics
Volume 9
Issue 2
10.3390/antibiotics9020067
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Article

Identification and Heterologous Expression of the Biosynthetic Gene Cluster Encoding the Lasso Peptide Humidimycin, a Caspofungin Activity Potentiator

by
Marina Sánchez-Hidalgo
*,
Jesús Martín
and
Olga Genilloud
Fundación MEDINA, Avenida del Conocimiento 34, 18016 Granada, Spain
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(2), 67; https://doi.org/10.3390/antibiotics9020067
Received: 22 January 2020 / Revised: 3 February 2020 / Accepted: 5 February 2020 / Published: 7 February 2020
(This article belongs to the Special Issue Antibiotics Acting on Cell Wall)
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Abstract

Humidimycin (MDN-0010) is a ribosomally synthesized and post-translationally modified peptide (RiPP) belonging to class I lasso peptides, and is structurally related to siamycins, which have been shown to have strong antimicrobial activities against Gram-positive bacteria and to possess anti-HIV activity. Humidimycin was isolated from the strain Streptomyces humidus CA-100629, and was shown to synergize the activity of the fungal cell wall inhibitor caspofungin. In this work, the biosynthetic gene cluster of humidimycin was identified by genome mining of S. humidus CA-100629, cloned by Gibson assembly, and heterologously expressed. View Full-Text
Keywords: Humidimycin; MDN-0010; lasso peptide; RiPP; Streptomyces humidus; genome mining; biosynthetic gene cluster; Gibson assembly; heterologous expression Humidimycin; MDN-0010; lasso peptide; RiPP; Streptomyces humidus; genome mining; biosynthetic gene cluster; Gibson assembly; heterologous expression
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MDPI and ACS Style

Sánchez-Hidalgo, M.; Martín, J.; Genilloud, O. Identification and Heterologous Expression of the Biosynthetic Gene Cluster Encoding the Lasso Peptide Humidimycin, a Caspofungin Activity Potentiator. Antibiotics 2020, 9, 67. https://doi.org/10.3390/antibiotics9020067

AMA Style

Sánchez-Hidalgo M, Martín J, Genilloud O. Identification and Heterologous Expression of the Biosynthetic Gene Cluster Encoding the Lasso Peptide Humidimycin, a Caspofungin Activity Potentiator. Antibiotics. 2020; 9(2):67. https://doi.org/10.3390/antibiotics9020067

Chicago/Turabian Style

Sánchez-Hidalgo, Marina, Jesús Martín, and Olga Genilloud. 2020. "Identification and Heterologous Expression of the Biosynthetic Gene Cluster Encoding the Lasso Peptide Humidimycin, a Caspofungin Activity Potentiator" Antibiotics 9, no. 2: 67. https://doi.org/10.3390/antibiotics9020067

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