Identification and Heterologous Expression of the Biosynthetic Gene Cluster Encoding the Lasso Peptide Humidimycin, a Caspofungin Activity Potentiator
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Antibiotics 2020, 9(2), 67; https://doi.org/10.3390/antibiotics9020067
Received: 22 January 2020 / Revised: 3 February 2020 / Accepted: 5 February 2020 / Published: 7 February 2020
(This article belongs to the Special Issue Antibiotics Acting on Cell Wall)
Humidimycin (MDN-0010) is a ribosomally synthesized and post-translationally modified peptide (RiPP) belonging to class I lasso peptides, and is structurally related to siamycins, which have been shown to have strong antimicrobial activities against Gram-positive bacteria and to possess anti-HIV activity. Humidimycin was isolated from the strain Streptomyces humidus CA-100629, and was shown to synergize the activity of the fungal cell wall inhibitor caspofungin. In this work, the biosynthetic gene cluster of humidimycin was identified by genome mining of S. humidus CA-100629, cloned by Gibson assembly, and heterologously expressed.
Keywords:
Humidimycin; MDN-0010; lasso peptide; RiPP; Streptomyces humidus; genome mining; biosynthetic gene cluster; Gibson assembly; heterologous expression
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MDPI and ACS Style
Sánchez-Hidalgo, M.; Martín, J.; Genilloud, O. Identification and Heterologous Expression of the Biosynthetic Gene Cluster Encoding the Lasso Peptide Humidimycin, a Caspofungin Activity Potentiator. Antibiotics 2020, 9, 67.
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