Next Article in Journal
Antibacterial Use of Macroalgae Compounds against Foodborne Pathogens
Next Article in Special Issue
Opportunist Coinfections by Nontuberculous Mycobacteria and Fungi in Immunocompromised Patients
Previous Article in Journal
Prevalence of Antibiotic-Resistant Pulmonary Tuberculosis in Bangladesh: A Systematic Review and Meta-Analysis
Previous Article in Special Issue
Mycogenic Metal Nanoparticles for the Treatment of Mycobacterioses
Article

Single Cell Analysis of Drug Susceptibility of Mycobacterium abscessus during Macrophage Infection

1
Department of Microbiology and Immunology, Yong Loo Lin School of Medcine, National University of Singapore, 5 Science Drive 2, Singapore 117545, Singapore
2
Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ 07110, USA
3
Department of Medical Sciences, Hackensack Meridian School of Medicine at Seton Hall University, Nutley, NJ 07110, USA
4
Immunology Programme, Life Sciences Institute, National University of Singapore, Singapore 117545, Singapore
*
Authors to whom correspondence should be addressed.
Antibiotics 2020, 9(10), 711; https://doi.org/10.3390/antibiotics9100711
Received: 21 September 2020 / Revised: 15 October 2020 / Accepted: 16 October 2020 / Published: 17 October 2020
(This article belongs to the Special Issue Mycobacterial Infections and Therapy)
Mycobacterium abscessus is an emerging health risk to immunocompromised individuals and to people with pre-existing pulmonary conditions. As M. abscessus possesses multiple mechanisms of drug resistance, treatments of M. abscessus are of poor efficacy. Therefore, there is an urgent need for new therapeutic strategies targeting M. abscessus. We describe an experimental system for screening of compounds for their antimicrobial activity against intracellular M. abscessus using flow cytometry and imaging flow cytometry. The assay allows simultaneous analysis of multiple parameters, such as proportion of infected host cells, bacterial load per host cell from the infected population, and host cell viability. We verified the suitability of this method using two antibiotics with known activity against M. abscessus: clarithromycin and amikacin. Our analysis revealed a high degree of infection heterogeneity, which correlated with host cell size. A higher proportion of the larger host cells is infected with M. abscessus as compared to smaller host cells, and infected larger cells have higher intracellular bacterial burden than infected smaller cells. Clarithromycin treatment has a more pronounced effect on smaller host cells than on bigger host cells, suggesting that heterogeneity within the host cell population has an effect on antibiotic susceptibility of intracellular bacteria. View Full-Text
Keywords: Mycobacterium abscessus; drug screen; single cell analysis Mycobacterium abscessus; drug screen; single cell analysis
Show Figures

Figure 1

MDPI and ACS Style

Brzostek, J.; Fatin, A.; Chua, W.H.; Tan, H.Y.; Dick, T.; Gascoigne, N.R.J. Single Cell Analysis of Drug Susceptibility of Mycobacterium abscessus during Macrophage Infection. Antibiotics 2020, 9, 711. https://doi.org/10.3390/antibiotics9100711

AMA Style

Brzostek J, Fatin A, Chua WH, Tan HY, Dick T, Gascoigne NRJ. Single Cell Analysis of Drug Susceptibility of Mycobacterium abscessus during Macrophage Infection. Antibiotics. 2020; 9(10):711. https://doi.org/10.3390/antibiotics9100711

Chicago/Turabian Style

Brzostek, Joanna, Amierah Fatin, Wen H. Chua, Hui Y. Tan, Thomas Dick, and Nicholas R.J. Gascoigne 2020. "Single Cell Analysis of Drug Susceptibility of Mycobacterium abscessus during Macrophage Infection" Antibiotics 9, no. 10: 711. https://doi.org/10.3390/antibiotics9100711

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop