Designed Antimicrobial Peptides for Topical Treatment of Antibiotic Resistant Acne Vulgaris
Abstract
:1. Introduction
2. Results
2.1. Designed Antimicrobial Peptides
2.2. C. acnes In Vitro Antibacterial Activity
2.3. Limited Mammalian Cytotoxicity
2.4. High Therapeutic Index
2.5. RP556 Activity in a Multidrug Resistant C. acnes Murine Model
3. Discussion
4. Materials and Methods
4.1. Designed Antimicrobial Peptides
4.2. Minimum Inhibitory Concentration (MIC)
4.3. Mammalian Cytotoxicity
4.4. Animals
4.5. In Vivo Efficacy Assessment of RP556 against Multidrug-Resistant HL043PA2 C. acnes
4.6. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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C. acnes | Antibiotic Sensitivity | dAMPs | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Tetra # | Ery # | Clind # | RP444 | RP551 | RP553 | RP554 | RP556 | RP557 | RP568 | |
ATCC6919 | S | S | S | 2 | 2 | 8 | 4 | 4 | 8 | >32 |
ATCC11827 | S | S | S | 8 | 2–4 | 16 | 8 | 8 | 8 | >32 |
HL007PA1 | R | R | R | 4 | 2 | 16 | 4 | 4 | 8 | >32 |
HL013PA1 | R | R | R | 4 | 2 | 8 | 2–4 | 2 | 2 | >32 |
HL038PA1 | R | R | R | 4 | 2 | 16 | 8 | 4 | 8 | >32 |
HL043PA1 | R | R | R | 4 | 2 | 16 | 4 | 4 | 8 | >32 |
HL043PA2 | R | R | R | 8 | 2 | 16 | 8 | 4 | 8 | >32 |
HL045PA1 | R | R | R | 4 | 2 | 16 | 4 | 4 | 4 | >32 |
HL053PA1 | R | R | R | 4 | 2 | 16 | 4 | 4 | 4 | >32 |
HL056PA1 | R | R | R | 4 | 2 | 16 | 4 | 2 | 4 | >32 |
HL072PA1 | R | R | R | 8 | 2–4 | 16 | 8 | 8 | 8 | >32 |
HL082PA2 | R | R | S | 2 | 2 | 16 | 8 | 2 | 2 | >32 |
dAMP | MIC (μg/mL) | EC10 (μg/mL) | Therapeutic Index | EC50 (μg/mL) | Therapeutic Index |
---|---|---|---|---|---|
RP444 | 8 | 13 | 1.63 | 20 | 2.5 |
RP551 | 2 | 16.6 | 8.3 | 34.3 | 17.2 |
RP553 | 16 | 238 | 39.7 | 268 | 16.8 |
RP554 | 8 | 19 | 2.38 | 31 | 3.88 |
RP556 | 4 | 276 | 70 | 519 | 130 |
RP557 | 8 | 246 | 30.8 | 281 | 35.1 |
RP568 | >32 | 146 | 4.56 | 229 | 7.16 |
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Woodburn, K.W.; Jaynes, J.; Clemens, L.E. Designed Antimicrobial Peptides for Topical Treatment of Antibiotic Resistant Acne Vulgaris. Antibiotics 2020, 9, 23. https://doi.org/10.3390/antibiotics9010023
Woodburn KW, Jaynes J, Clemens LE. Designed Antimicrobial Peptides for Topical Treatment of Antibiotic Resistant Acne Vulgaris. Antibiotics. 2020; 9(1):23. https://doi.org/10.3390/antibiotics9010023
Chicago/Turabian StyleWoodburn, Kathryn W., Jesse Jaynes, and L. Edward Clemens. 2020. "Designed Antimicrobial Peptides for Topical Treatment of Antibiotic Resistant Acne Vulgaris" Antibiotics 9, no. 1: 23. https://doi.org/10.3390/antibiotics9010023