The Effect of 2-Thiocyanatopyridine Derivative 11026103 on Burkholderia Cenocepacia: Resistance Mechanisms and Systemic Impact
Abstract
:1. Introduction
2. Results and Discussion
2.1. Selection and Genetic Characterization of B. cenocepacia Mutants Resistant to 11026103
2.2. Resistance of B. cenocepacia to 11026103 Is Solely Efflux-Mediated
2.3. Knockdowns in Transport and Metabolic Genes Increase Resistance to 11026103
2.4. Transcriptomic Response of B. cenocepacia Elicited by 11026103
3. Materials and Methods
3.1. Bacterial Strains, Plasmids, and Growth Conditions
3.2. Chemical Mutagenesis
3.3. MIC Determination
3.4. Construction of pSCrhaB2 Derivatives and Genetic Modification of B. cenocepacia
3.5. Whole-Genome Sequencing and Data Analysis
3.6. RNA-Seq and Data Analysis
3.7. Competitive Fitness Assay and Data Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Nunvar, J.; Hogan, A.M.; Buroni, S.; Savina, S.; Makarov, V.; Cardona, S.T.; Drevinek, P. The Effect of 2-Thiocyanatopyridine Derivative 11026103 on Burkholderia Cenocepacia: Resistance Mechanisms and Systemic Impact. Antibiotics 2019, 8, 159. https://doi.org/10.3390/antibiotics8040159
Nunvar J, Hogan AM, Buroni S, Savina S, Makarov V, Cardona ST, Drevinek P. The Effect of 2-Thiocyanatopyridine Derivative 11026103 on Burkholderia Cenocepacia: Resistance Mechanisms and Systemic Impact. Antibiotics. 2019; 8(4):159. https://doi.org/10.3390/antibiotics8040159
Chicago/Turabian StyleNunvar, Jaroslav, Andrew M. Hogan, Silvia Buroni, Svetlana Savina, Vadim Makarov, Silvia T. Cardona, and Pavel Drevinek. 2019. "The Effect of 2-Thiocyanatopyridine Derivative 11026103 on Burkholderia Cenocepacia: Resistance Mechanisms and Systemic Impact" Antibiotics 8, no. 4: 159. https://doi.org/10.3390/antibiotics8040159
APA StyleNunvar, J., Hogan, A. M., Buroni, S., Savina, S., Makarov, V., Cardona, S. T., & Drevinek, P. (2019). The Effect of 2-Thiocyanatopyridine Derivative 11026103 on Burkholderia Cenocepacia: Resistance Mechanisms and Systemic Impact. Antibiotics, 8(4), 159. https://doi.org/10.3390/antibiotics8040159