Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection
AbstractHemolytic–uremic syndrome is a life-threating disease most often associated with Shiga toxin-producing microorganisms like Escherichia coli (STEC), including E. coli O157:H7. Shiga toxin is encoded by resident prophages present within this bacterium, and both its production and release depend on the induction of Shiga toxin-encoding prophages. Consequently, treatment of STEC infections tend to be largely supportive rather than antibacterial, in part due to concerns about exacerbating such prophage induction. Here we explore STEC O157:H7 prophage induction in vitro as it pertains to phage therapy—the application of bacteriophages as antibacterial agents to treat bacterial infections—to curtail prophage induction events, while also reducing STEC O157:H7 presence. We observed that cultures treated with strictly lytic phages, despite being lysed, produce substantially fewer Shiga toxin-encoding temperate-phage virions than untreated STEC controls. We therefore suggest that phage therapy could have utility as a prophylactic treatment of individuals suspected of having been recently exposed to STEC, especially if prophage induction and by extension Shiga toxin production is not exacerbated. View Full-Text
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Howard-Varona, C.; Vik, D.R.; Solonenko, N.E.; Li, Y.-F.; Gazitua, M.C.; Chittick, L.; Samiec, J.K.; Jensen, A.E.; Anderson, P.; Howard-Varona, A.; Kinkhabwala, A.A.; Abedon, S.T.; Sullivan, M.B. Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection. Antibiotics 2018, 7, 101.
Howard-Varona C, Vik DR, Solonenko NE, Li Y-F, Gazitua MC, Chittick L, Samiec JK, Jensen AE, Anderson P, Howard-Varona A, Kinkhabwala AA, Abedon ST, Sullivan MB. Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection. Antibiotics. 2018; 7(4):101.Chicago/Turabian Style
Howard-Varona, Cristina; Vik, Dean R.; Solonenko, Natalie E.; Li, Yueh-Fen; Gazitua, M. C.; Chittick, Lauren; Samiec, Jennifer K.; Jensen, Aubrey E.; Anderson, Paige; Howard-Varona, Adrian; Kinkhabwala, Anika A.; Abedon, Stephen T.; Sullivan, Matthew B. 2018. "Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection." Antibiotics 7, no. 4: 101.
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