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Antibiotics 2018, 7(3), 70; https://doi.org/10.3390/antibiotics7030070

Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae

1
Department of Biochemistry Microbiology and Immunology, 2D01 Health Science Building, 107 Wiggins Road, University of Saskatchewan, Saskatoon, SK S7N5E5, Canada
2
Vaccine and Infectious Disease Organization—International Vaccine Centre, 120 Veterinary Road, Saskatoon, SK S7N5E3, Canada
3
Aquila Diagnostic Systems Inc., 4-034 National Institute for Nanotechnology, 11421 Saskatchewan Drive, Edmonton, AB T6G2M9, Canada
4
National Microbiology Laboratory, Streptococcus and STI Unit, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada
5
Department of Computer Science, 176 Thorvaldson Building, 110 Science Place, University of Saskatchewan, Saskatoon, SK S7N5C9, Canada
*
Author to whom correspondence should be addressed.
Received: 19 July 2018 / Revised: 1 August 2018 / Accepted: 2 August 2018 / Published: 4 August 2018
(This article belongs to the Special Issue Multi-Drug Resistant Neisseria gonorrhoeae)
Full-Text   |   PDF [271 KB, uploaded 7 August 2018]

Abstract

Eleven primer pairs were developed for the identification of Neisseria gonorrhoeae. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 N. gonorrhoeae isolates and 40 other Neisseria or non-Neisseria species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50 N. gonorrhoeae cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for N. gonorrhoeae diagnosis. View Full-Text
Keywords: Neisseria gonorrhoeae; molecular diagnosis; RT-PCR; hydrogel Neisseria gonorrhoeae; molecular diagnosis; RT-PCR; hydrogel
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Perera, S.R.; Taheri, A.; Khan, N.H.; Parti, R.P.; Stefura, S.; Skiba, P.; Acker, J.P.; Martin, I.; Kusalik, A.; Dillon, J.-A.R. Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae. Antibiotics 2018, 7, 70.

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