Next Article in Journal / Special Issue
Screening of E. coli β-clamp Inhibitors Revealed that Few Inhibit Helicobacter pylori More Effectively: Structural and Functional Characterization
Previous Article in Journal
Sperm Quality during Storage Is Not Affected by the Presence of Antibiotics in EquiPlus Semen Extender but Is Improved by Single Layer Centrifugation
Open AccessFeature PaperArticle

Establishing a System for Testing Replication Inhibition of the Vibrio cholerae Secondary Chromosome in Escherichia coli

1
LOEWE Center for Synthetic Microbiology-SYNMIKRO, Philipps-Universität Marburg, Marburg 35032, Germany
2
School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, Manchester M1 7DN, UK
*
Author to whom correspondence should be addressed.
N.S., S.M., T.S. and F.S.K. contributed equally to this work.
Academic Editor: Anders Løbner-Olesen
Antibiotics 2018, 7(1), 3; https://doi.org/10.3390/antibiotics7010003
Received: 30 October 2017 / Revised: 5 December 2017 / Accepted: 20 December 2017 / Published: 23 December 2017
(This article belongs to the Special Issue Bacterial DNA Replication and Replication Inhibitors)
Regulators of DNA replication in bacteria are an attractive target for new antibiotics, as not only is replication essential for cell viability, but its underlying mechanisms also differ from those operating in eukaryotes. The genetic information of most bacteria is encoded on a single chromosome, but about 10% of species carry a split genome spanning multiple chromosomes. The best studied bacterium in this context is the human pathogen Vibrio cholerae, with a primary chromosome (Chr1) of 3 M bps, and a secondary one (Chr2) of about 1 M bps. Replication of Chr2 is under control of a unique mechanism, presenting a potential target in the development of V. cholerae-specific antibiotics. A common challenge in such endeavors is whether the effects of candidate chemicals can be focused on specific mechanisms, such as DNA replication. To test the specificity of antimicrobial substances independent of other features of the V. cholerae cell for the replication mechanism of the V. cholerae secondary chromosome, we establish the replication machinery in the heterologous E. coli system. We characterize an E. coli strain in which chromosomal replication is driven by the replication origin of V. cholerae Chr2. Surprisingly, the E. coli ori2 strain was not inhibited by vibrepin, previously found to inhibit ori2-based replication. View Full-Text
Keywords: chromosome engineering; replication initiation; drug development chromosome engineering; replication initiation; drug development
Show Figures

Figure 1

MDPI and ACS Style

Schallopp, N.; Milbredt, S.; Sperlea, T.; Kemter, F.S.; Bruhn, M.; Schindler, D.; Waldminghaus, T. Establishing a System for Testing Replication Inhibition of the Vibrio cholerae Secondary Chromosome in Escherichia coli. Antibiotics 2018, 7, 3.

AMA Style

Schallopp N, Milbredt S, Sperlea T, Kemter FS, Bruhn M, Schindler D, Waldminghaus T. Establishing a System for Testing Replication Inhibition of the Vibrio cholerae Secondary Chromosome in Escherichia coli. Antibiotics. 2018; 7(1):3.

Chicago/Turabian Style

Schallopp, Nadine; Milbredt, Sarah; Sperlea, Theodor; Kemter, Franziska S.; Bruhn, Matthias; Schindler, Daniel; Waldminghaus, Torsten. 2018. "Establishing a System for Testing Replication Inhibition of the Vibrio cholerae Secondary Chromosome in Escherichia coli" Antibiotics 7, no. 1: 3.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop