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Article

Silent Outbreaks of Candida duobushaemulonii in a Pediatric Ward in Brazil

by
Daniel Wagner de Castro Lima Santos
1,2,
Bram Spruijtenburg
3,4,5,
Eelco F. J. Meijer
3,4,5,
Dayse Azevedo Coelho de Souza
6,7,
Conceição de Maria Pedrozo e Silva de Azevedo
6,7 and
Jacques F. Meis
3,4,8,*
1
Instituto D’Or de Pesquisa e Ensino (IDOR), São Luís 65076-820, MA, Brazil
2
Hospital Universitário, Universidade Federal do Maranhão, São Luís 65020-070, MA, Brazil
3
Department of Medical Microbiology, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands
4
Radboudumc-CWZ Center of Expertise for Mycology, 6532 SZ Nijmegen, The Netherlands
5
Department of Medical Microbiology and Immunology, Canisius-Wilhelmina Hospital/Dicoon, 6532 SZ Nijmegen, The Netherlands
6
Programa de Pós Graduação em Ciências da Saúde, Universidade Federal do Maranhão (UFMA), São Luís 65020-070, MA, Brazil
7
Hospital de Cancer Aldenora Bello(HCAB), São Luís 65031-630, MA, Brazil
8
Institute of Translational Research, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and Excellence Center for Medical Mycology, University of Cologne, 50923 Cologne, Germany
*
Author to whom correspondence should be addressed.
Antibiotics 2026, 15(3), 237; https://doi.org/10.3390/antibiotics15030237
Submission received: 29 January 2026 / Revised: 18 February 2026 / Accepted: 23 February 2026 / Published: 25 February 2026
(This article belongs to the Special Issue Prevention and Antimicrobial Surveillance of Hospital-Associated Infections)
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Abstract

Background: While Candida auris is well known to cause hospital outbreaks, other species in the C. haemulonii complex are less well documented but gained attention as opportunistic pathogens. Only one documented outbreak has been published. We describe the second, silent, fungemia outbreak due to antifungal-susceptible C. duobushaemulonii. Methods: We retrospectively genotyped six C. duobushaemulonii bloodstream isolates, collected in a 4-month-period in 2022 (n = 4) and during a week in 2024 (n = 2) in pediatric patients in Brazil. Whole genome sequencing (WGS) was done and compared to n = 33 publicly available genomes, including four cases from an outbreak in Panama. Antifungal susceptibility was performed with the reference CLSI method. Results: MALDI-TOF-MS identified isolates as either C. pseudohaemulonii or C. duobushaemulonii albeit with low scores. ITS sequence analyses confirmed all isolates as C. duobushaemulonii. WGS proved the presence of an outbreak among four pediatric patients in 2022 and a genetically distinct cluster of two cases in 2024. All six isolates were susceptible to azoles and echinocandins and were interpreted as being resistant to amphotericin B with a MIC at breakpoint of 2 µg/mL. Conclusions: This study describes the second documented outbreak due to the rare yeast C. duobushaemulonii, belonging to the C. haemulonii species complex, during 2022–2024 in patients admitted to a pediatric oncology ward in a Brazilian hospital.

1. Introduction

The recent emergence of Candida auris, a global fungal public health threat [1], has drawn attention to seven closely related, less well-known species in the C. haemulonii species complex that include C. haemulonii sensu strictu, C. duobushaemulonii, C. pseudohaemulonii, C. vulturna, C. haemulonii var. vulnera [2,3], and the recently identified species C. khanbhai [4] and C. molenica [5]. Invasive infections other than C. auris are uncommon and are mainly encountered in India, China and the Americas, including sporadic nosocomial outbreaks involving C. haemulonii, C. duobushaemulonii and C. vulturna [3]. C. duobushaemulonii, previously classified as C. haemulonii group II, was re-categorized as a separate species in 2012 [6]. While C. haemulonii was already described in 1962 [7], C. duobushaemulonii, the second species in the C. haemulonii species complex, was retrospectively found in a yeast culture collection at the US Centers for Disease Control (CDC) as early as 1990 [8]. Although cases of C. duobushaemulonii infection have been rare to date, they have been mainly reported in the USA, Latin America, India, and China [8,9,10,11,12,13]. These studies either mentioned no clinical background or included only adults in the study population except one study which had three pediatric patients among 10 cases with fungemia [9]. A study on rare fungemias in children in Taiwan reported only one non-oncologic patient with C. duobushaemulonii among 46 cases [14]. C. duobushaemulonii has several virulence factors in common with C. auris which aid in medical-device colonization, antifungal and disinfectant tolerance, osmotic stress and thermotolerance, all contributing to its persistence in hospital environments and risks of nosocomial outbreaks [15,16].
A series of invasive C. duobushaemulonii infections were identified in Panama in 2016 concomitant with cases of C. auris [8,9,17] and subsequent epidemiological investigations, including whole genome sequencing (WGS), confirmed the first outbreak with this sibling of C. auris in 2020 [8]. By using high-resolution WGS analysis and a large set of publicly available genomes, we aimed to study two in-hospital transmission events with C. duobushaemulonii in a pediatric oncology ward in Northeast Brazil, where earlier also an outbreak with C. vulturna occurred [18].

2. Results

2.1. Patients and Isolate Identification

The clinical and laboratory parameters of the six patients are summarized in Table 1 and Table 2. The mean age of the patients was 6.6 years (range 3 to 12 years), and 50% were male. The majority suffered from acute lymphoblastic leukemia as an underlying condition. A central venous catheter was present in all patients at the time of diagnosis, and two patients received total parenteral nutrition. Five patients were treated with broad-spectrum antimicrobial therapy at the time of fungemia. Fever and chills were the most common presenting features and were observed in five patients (83%). Concomitant bacteraemia was detected in two patients, involving Acinetobacter baumannii in one case and Staphylococcus epidermidis in the other. Severe neutropenia (<100 cells/mm3) was present in two patients at the time of diagnosis of fungemia. All patients remained haemodynamically stable during targeted antifungal therapy with intravenous fluconazole (12 mg/kg followed by 6 mg/kg once daily) and follow up blood cultures remained negative. No cases of deep-seated infections were detected. The 30-day mortality was 67% (4/6 patients), most likely related to the severe underlying diseases. We suspected an outbreak because the first (2022) and second (2024) group of patients were timely restricted and had the same provisional pathogen isolated. The first outbreak occurred in the same period as a larger outbreak with C. vulturna. We considered this outbreak to be silent because it was only uncovered several months after the event.
The six clinical isolates were initially misidentified as C. pseudohaemulonii/C. duobushaemulonii (with low scores of <1.7) or retrieved no database match with MALDI-TOF-MS but were later determined to be C. duobushaemulonii confirmed by sequencing of the ITS region (Figure 1).

2.2. Outbreak Investigation

All isolates were sent for Illumina sequencing and results are detailed in Supplementary Table S1. By conducting WGS SNP analysis on all six C. duobushaemulonii blood culture isolates and 33 controls from eight countries, an overall low genetic diversity was found with less than 1500 SNPs difference. The six isolates from the current investigated hospital clustered in two distinct branches, a tight cluster of four isolates occurring in 2022 and one cluster in 2024 respectively (Figure 2). Within the two groups, isolates were virtually identical (2 SNPs difference at most), while between isolates of the two identified outbreaks the variation was 462 SNPs. When compared to the nearest control isolates for both clusters, the genetic distance was at least 100 SNPs. A cluster of four isolates, from different patients, from a published outbreak in Panama formed a cluster on the phylogenetic WGS tree, with two identical isolates (0 SNPs) and two with less than 5 SNPs difference. No aneuploidy or large-scale copy number variation was found in the six isolates involved in the current outbreaks. By including control isolates from other countries, there were no monophyletic branches visible based on the country or continent of origin.

2.3. Resistance Investigation

By conducting AFST according to CLSI microbroth dilution, isolates were found to be resistant to amphotericin B (2 µg/mL) and susceptible for fluconazole (4–8 μg/mL) and echinocandins (≤0.125 μg/mL) (Table 3). Genomic inspection of known, resistance-associated genes ERG3, ERG6, ERG11 and FKS1 did not show any missense mutations when compared to the reference genome.

3. Discussion

C. duobushaemulonii, previously classified as C. haemulonii group II, was re-categorized and named a separate species in 2012 [6], as part of the C. haemulonii species complex that also comprises C. haemulonii senso strictu, C. haemulonii var. vulnera, and C. pseudohaemulonii [2]. Retrospective studies of archived isolates found that the earliest recorded C. duobushaemulonii were from 1990, stored in the CDC culture collection [8], and from 1996 in Spain [19]. C. duobushaemulonii is an opportunistic yeast that has been identified in various environments and from clinical settings such as deep cutaneous infections [20], recurrent vulvovaginal candidiasis [21], chronic wound infections [22] and candidemia [9]. Additionally, antifungal susceptibility testing has shown that C. duobushaemulonii is often resistant to amphotericin B and fluconazole [6,9]. Most cases of C. duobushaemulonii infections have been reported in the USA, Latin America and Asia [8,9,10,11,12,13] with only sporadic reports from other countries suggesting a geographic restriction. As seen with many other rare Candida species, C. duobushaemulonii infections are likely underreported because identifying closely related species in the complex is challenging. Phenotypic identification systems can misidentify C. duobushaemulonii as closely related species in the C. haemulonii species complex. Although MALDI-TOF-MS databases have improved the ability to differentiate C. auris and closely related species, C. duobushaemulonii is sometimes misidentified as C. haemulonii or C. pseudohaemulonii [23], as was the case in this report.
A recent review [24] reported that China and especially Brazil were the countries with the highest numbers of C. haemulonii species complex infections including C. duobushaemulonii. The prevalence rates of the C. haemulonii species complex in 12 Brazilian medical centers rose, in the period 2008 to 2013, from 0.9% to 1.7% (2014 to 2019) [25]. A more recent survey of bloodstream infections caused by rare Sacharomycetales in 28 medical centers across Brazil, showed that C. haemulonii sensu stricto (14%) and C. duobushaemulonii (12%) made up more than 25% among rare isolates. Stratified by two eight-year periods, C. duobushaemulonii increased by 400% between the years 2007–2015 versus 2016–2023 [26].
In the most recent Brazilian study, C. duobushaemulonii had an increased fluconazole MIC90 of 16 µg/mL, and a high amphotericin B MIC90 of ≥8 µg/mL similar as earlier data from Brazil [27,28]. A study from the CDC of n = 55 C. duobushaemulonii from Latin America showed that 93% had elevated MICs of amphotericin B and, similar as the Brazilian studies, 87% had fluconazole MICs of ≤16 μg/mL [8]. Interestingly, n = 5 isolates with an MIC of 256 μg/mL had the Y132F mutation in the ERG11 gene (ERG11Y132F), with concomitant elevated voriconazole MICs of 1–2 μg/mL. All abovementioned studies used CLSI methods and thus were comparable with our results. The isolates responsible for the two outbreaks in our study had slightly elevated MICs of amphotericin B and fluconazole. Chromosomal duplications linked to resistance and resistance-associated genes were inspected, which showed wildtype ERG6 and ERG11, which is in line with earlier reports and suggests that susceptibility patterns may have regional variability. In contrast, the first outbreak described in Panama was caused by a fluconazole resistant clone exhibiting ERG11Y132F, a similar substitution recently reported in China [16]. In contrast, outbreaks in two other Brazilian states with C. auris, the most prominent species in the complex, also showed low azole and amphotericin B MICs for clades I and IV, which are known for their resistant phenotype [29,30]. A similar observation was made for C. vulturna, another species in the complex, also showing low MICs of fluconazole and amphotericin B [18]. The difference between Brazil and other countries regarding the pressure for developing resistance for these antifungals remains unclear. Unfortunately, no environmental isolates could be retrieved, making the source of the current and previous outbreak unknown [18]. Source tracking is notoriously difficult especially for outbreaks with rare yeasts, although medical devices and contaminated hands of health care workers are occasionally involved [3].
Whole-genome sequencing SNP analysis demonstrated two clusters of clonally related C. duobushaemulonii blood isolates within a pediatric hospital in northeast Brazil which had previously reported an outbreak of C. vulturna [18]. By comparing the present outbreaks with published C. duobushaemulonii WGS data from eight countries, an overall low genetic diversity was found with less than 1500 SNPs, which is concordant with previous reports [8]. Interestingly, the same is also found for related species in the complex, like C. haemulonii, C. vulturna, or within the different C. auris clades [8,31], suggesting that the common ancestors of these populations are a recent expansion within the human population. Within the two clusters in the present outbreak, isolates were virtually identical (2 SNPS at most) and between the clusters from 2022 and 2024 there was a >450 SNP difference. Despite a two-year difference between the two outbreaks in the same hospital, this genetic difference of more than 450 SNPs is unlikely to be accrued within this time period, suggesting two separate introductions and nosocomial transmission events. To date, only one other, retrospectively identified, clonal transmission event for C. duobushaemulonii has been reported, consisting of four fluconazole resistant isolates, exhibiting ERG11Y132F, in a single Panamanian hospital. The latter cluster consisted of an identical blood and nail isolate (0 SNPs), and two blood isolates separated by <5 SNPs [8].
The hospital in Panama recorded n = 14 cases of C. duobushaemulonii between November 2016–May 2017 [9], but an outbreak was only confirmed for four cases [8]. Concomitant infections were observed, overlapping in time with other species in the C. haemulonii species complex and C. auris [8,17], similar as the observed clusters of C. duobushaemulonii and C. vulturna in Brazil [18]. It is not unimaginable that patients had undetected mixed fungemia with more than one species from the C. haemulonii species complex. This has been described at least once in a patient repatriated from Vietnam to Australia with a double infection/colonization with C. duobushaemulonii and C. auris [32].
The main limitation of this study was the retrospective nature and the failure to find potential environmental sources of the outbreak. Another limitation of this research is the lack of benchmarking data. With only one previous outbreak described in the literature [8]—and without a specified setting—the ability to compare our results remains limited. Consequently, this study should be viewed as an exploratory step in understanding the transmission dynamics of this poorly described sibling of C. auris.

4. Materials and Methods

4.1. Study Population and Isolates

The outbreak occurred in Hospital de Cancer Aldenora Bello, a reference center in São Luís, Maranhão, which is a 150-bed specialized teaching hospital that focuses on oncology. The hospital admits approximately 500 pediatric cancer patients annually. Six pediatric patients were identified with bloodstream infection caused by C. duobushaemulonii. Clinical and laboratory patient information was retrospectively collected from chart reviews. Four cases occurred over a three-month period in 2022, and two additional cases were identified two years later, during a one-month period in 2024. Yeasts were isolated from blood cultures taken from febrile patients and processed with the BACTEC 9240 system (Becton Dickinson, Franklin Lakes, NJ, USA).

4.2. Species Identification

Isolates were processed for identification with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) (MALDI Biotyper version 3.1, database MBT 7854 MSP library RUO; Bruker Daltonik, Bremen, Germany). Spectra were obtained from cultures grown for 48 h on Sabouraud agar. A protein extraction protocol with sequential ethanol, formic acid, and acetonitrile was used [33]. All extracts were overlaid with 2 μL of matrix (saturated solution of α-cyano-4-hydroxycinnamic acid in 50% acetonitrile–2.5% trifluoroacetic acid; Bruker Daltonik) and air dried at room temperature to acquire spectra. A score of ≥2 equaled identification to the species level, and a score between 1.7 and 1.9 means identification to the genus level. Final species identification was obtained by sequencing of the ITS2 region with ITS-1 and ITS-4 primers as previously described [8]. DNA of fresh colonies was extracted using the MagNA Pure 96 instrument and the MagNA Pure DNA and Viral NA Small Volume Kit polymerase (Roche Diagnostics, Mannheim, Germany) according to manufacturer’s protocol. In short, the ITS2 region was amplified with a thermocycler (Biometra, Westburg, Germany) using 1 U FastStart Taq polymerase (all Roche Diagnostics, Mannheim, Germany), 1 × FastStart Taq polymerase buffer, MgCl2 (3 mM) polymerase (all Roche Diagnostics), forward and reverse primers (5 µM) and isolated DNA. Amplicons were purified using the Ampliclean protocol (NimaGen, Nijmegen, The Netherlands). Sanger sequencing was conducted on a 3500XL genetic analyzer (Applied Biosystems, Foster City, CA, USA). Control sequences of the Metschnikowiaceae clade were retrieved from the National Center for Biotechnology Information (NCBI) nucleotide database and included C. vulturna CVDH05 (OQ519941.1), C. duobushaemulonii (JX459666.1), C. pseudohaemulonii (NR_163771.1), C. haemulonii (AY500375.1), C. haemulonii var. vulnera (JX459686.1), C. auris (PP178707.1), C. khanbhai (OP626788.1), and C. lusitaniae (AF172262.1). Sequences were aligned and a phylogenetic tree was built and visualized as described earlier [8]. Generated ITS sequences of the current study were deposited to the NCBI Genbank database (Accession numbers: PX844698-PX844703).

4.3. Antifungal Susceptibility Testing (AFST)

In vitro AFST according to Clinical and Laboratory Standards Institute (CLSI) M27 standard was performed for amphotericin B (Bristol Meyers Squibb, Rotterdam, The Netherlands), fluconazole (Sigma-Aldrich, Amsterdam, The Netherlands), itraconazole (Janssen Cilag, Breda, the Netherlands), voriconazole (Pfizer, New York, NY, USA), posaconazole (Merck, Darmstadt, Germany), isavuconazole (Basilea Pharmaceutica, Basel, Switzerland), anidulafungin (Astellas Pharma, Tokyo, Japan) and micafungin (Astellas Pharma) [34]. Briefly, colonies were diluted in RPMI medium to a final concentration of 1 × 103–5 × 105 CFU/mL obtained with a Genesys 20 Spectrophotometer (Thermo Fisher, Waltham, MA, USA). Microbroth dilution plates were read visually after incubation at 35 °C for 24 h. Minimum inhibitory concentrations (MICs) were defined as the lowest antifungal concentration that reduced growth by 50% or more when compared to the growth control, except for amphotericin B with 100% growth reduction. Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258 were used as quality control strains. For many rare yeasts, including C. duobushaemulonii, no breakpoints are available. Therefore MICs were interpreted with tentative CDC breakpoints that were established for Candida auris (https://www.cdc.gov/candida-auris/hcp/laboratories/antifungal-susceptibility-testing.html, accessed on 18 February 2026) with the resistance breakpoint for amphotericin B of ≥2 µg/mL, for fluconazole of ≥32 µg/mL and echinocandins (anidulafungin or micafungin) of ≥4 µg/mL.

4.4. Whole Genome Sequencing (WGS) Single Nucleotide Polymorphism (SNP) Analysis

All isolates were cultured at 35 °C for 24 h on Sabouraud dextrose agar (SDA) (Oxoid, Hampshire, UK). DNA was extracted and purified with the MagNA Pure 96 systems according to manufacturers’ instructions as described earlier [35]. Next, extracted DNA was treated with 5 µg/µL of RNase A (Merck KGaA, Darmstadt, Germany) at room temperature for one hour, following a second purification with the MagNA Pure 96 system. Final DNA concentration was assessed with Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) using the double DNA (dsDNA) high-sensitivity option. Genomic libraries were prepared and sequenced with the Illumina Novaseq 6000 platform (Illumina, San Diego, CA, USA) with two times 150 bp paired-end-read mode at Eurofins Genomics (Ebersberg, Germany). Genomic reads were aligned to the C. duobushaemulonii reference genome B09383 (GCA_002926085.1) and a SNP analysis was conducted as previously described [36]. Briefly, read alignment was performed using BWA v0.7.19 and generated BAM files were filtered based on the MAPQ score and if reads were properly paired. Variants were called using Freebayes v1.3.9 and subsequently filtered in a combined VCF file using a minimum QUAL score of 100 and a minimum read depth of 20 for each isolate. The phylogeny was visualized with MEGA11 v11.0.10 by constructing a neighbor-joining tree and iTOL v6. Previously, this SNP calling pipeline was validated for C. auris by using a benchmark dataset of clade I isolates [35]. Up to five randomly selected control isolates per country from the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) were included in the SNP analysis, in addition to four Panamanian isolates involved in an earlier outbreak [8] (Supplementary Table S2). Aneuploidy and large-scale copy number variation was assessed with QualiMap BamQC v2.3. With BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi, accessed on 23 May 2025) antifungal resistance-associated genes ERG11 (CXQ87_003531), FKS1 (CXQ87_002203), ERG6 (CXQ87_004088), ERG3 (CXQ87_003761) were located in the B09383 reference genome and isolates were visually inspected for missense mutations using Integrated Genomics Viewer. Missense mutations were defined as position for which isolates had a minimum read depth of 15 times, with a nucleotide variant present in more than 90% of the reads. Raw read data generated during this study have been submitted to the NCBI SRA database under BioProject ID PRJNA1279651.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/antibiotics15030237/s1, Table S1: Overview of sequencing results for the six C. duobushaemulonii isolates collected in the current study. Table S2: C. duobushaemulonii control isolates (n = 33) used in single nucleotide polymorphism (SNP) analysis.

Author Contributions

Conceptualization, C.d.M.P.e.S.d.A., D.W.d.C.L.S. and J.F.M.; Acquisition of data, B.S. and D.A.C.d.S.; Writing—original draft preparation, B.S. and J.F.M.; Molecular analysis, B.S.; Resources, E.F.J.M.; Supervision, C.d.M.P.e.S.d.A., D.W.d.C.L.S., E.F.J.M. and J.F.M.; Writing—review and editing, D.W.d.C.L.S., B.S., D.A.C.d.S., E.F.J.M., C.d.M.P.e.S.d.A. and J.F.M. All authors have read and agreed to the published version of the manuscript.

Funding

This work received support from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Processo: 408891/2022-0 and the Canisius-Wilhelmina Hospital (grant number CWZ_001421). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Institutional Review Board Statement

The study was conducted in accordance with the Declaration of Helsinki, and approved by the Institutional Research Ethics Committee of the CEUMA University in compliance with the requirements of Resolution 466/2012 of the National Health Council, on 27 February 2025 with approval number 7.416.253.

Informed Consent Statement

Patient consent was waived due to de-identification of patients.

Data Availability Statement

The raw WGS read data have been submitted to the NCBI Sequence Read Archive (BioProject ID: PRJNA1279651) and ITS sequences have been submitted to the NCBI Genbank database (Accession numbers: PX844698-PX844703).

Conflicts of Interest

Eelco F. J. Meijer received research grants from Mundipharma and Scynexis, is in the scientific advisory board for Pfizer and has received speaker fees from Gilead Sciences. All other authors declare that there are no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Abbreviations

The following abbreviations are used in this manuscript:
AFSTAntifungal susceptibility testing
CDCUS Centers for Disease Control
CLSIClinical and Laboratory Standards Institute
ITSInternally Transcribed Spacer
IVIntravenous
MALDI-TOF-MSMatrix-assisted laser desorption/ionization-time of flight mass spectrometry
MICMinimum inhibitory concentration
NCBINational Center for Biotechnology Information
SNPSingle-nucleotide polymorphism
WGSWhole genome sequencing

References

  1. Lionakis, M.S.; Chowdhary, A. Candida auris Infections. N. Engl. J. Med. 2024, 391, 1924–1935. [Google Scholar] [CrossRef] [PubMed]
  2. Cao, C.; Bing, J.; Liao, G.; Nobile, C.J.; Huang, G. Candida haemulonii species complex: Emerging fungal pathogens of the Metschnikowiaceae clade. Zoonoses 2023, 3, 43. [Google Scholar] [CrossRef] [PubMed]
  3. Harchand, R.; Spruijtenburg, B.; Meijer, E.F.J.; de Groot, T.; Rudramurthy, S.M.; Meis, J.F. Candida vulturna, the next fungal menace? A narrative review. Mycoses 2025, 68, e70070. [Google Scholar] [CrossRef]
  4. de Jong, A.W.; Al-Obaid, K.; Mohd Tap, R.; Gerrits van den Ende, B.; Groenewald, M.; Joseph, L.; Ahmad, S.; Hagen, F. Candida khanbhai sp. nov., a new clinically relevant yeast within the Candida haemulonii species complex. Med. Mycol. 2023, 61, myad009. [Google Scholar] [CrossRef] [PubMed]
  5. Zandijk, A.; van der Bruggen, T.; Sipiczki, M.; Tissing, W.J.E.; Wolfs, T.F.W.; Gerrits van den Ende, B.; Groenewald, M.; Hagen, F. Expansion of the Candidozyma haemuli Species Complex—The Novel Species Candidozyma molenica, Isolated from Clinical and Environmental Sources. Mycopathologia 2025, 190, 81. [Google Scholar] [CrossRef]
  6. Cendejas-Bueno, E.; Kolecka, A.; Alastruey-Izquierdo, A.; Theelen, B.; Groenewald, M.; Kostrzewa, M.; Cuenca-Estrella, M.; Gómez-López, A.; Boekhout, T. Reclassification of the Candida haemulonii complex as Candida haemulonii (C. haemulonii group I.), C. duobushaemulonii sp. nov. (C. haemulonii group II), and C. haemulonii var. vulnera var. nov.: Three multi resistant human pathogenic yeasts. J. Clin. Microbiol. 2012, 50, 3641–3651. [Google Scholar] [CrossRef]
  7. van Uden, N.; Kolipinski, M.C. Torulopsis haemulonii nov. spec., a yeast from the Atlantic Ocean. Antonie Van Leeuwenhoek 1962, 28, 78–80. [Google Scholar] [CrossRef]
  8. Gadi, L.; Muñhoz, J.F.; Seith, M.; Wagner, D.; Berkow, E.L.; Forsberg, K.; Jackson, B.R.; Ramos-Castro, R.; Escandón, P.; Dolande, M.; et al. Understanding the Emergence of Multidrug-Resistant Candida: Using Whole-Genome Sequencing to Describe the Population Structure of Candida haemulonii Species Complex. Front. Genet. 2020, 11, 554. [Google Scholar] [CrossRef]
  9. Ramos, R.; Caceres, D.H.; Perez, M.; Garcia, N.; Castillo, W.; Santiago, E.; Borace, J.; Lockhart, S.R.; Berkow, E.L.; Hayer, L.; et al. Emerging Multidrug-Resistant Candida duobushaemulonii Infections in Panama Hospitals: Importance of Laboratory Surveillance and Accurate Identification. J. Clin. Microbiol. 2018, 56, e00371-18. [Google Scholar] [CrossRef]
  10. De Almeida, J.N., Jr.; Assy, J.G.; Levin, A.S.; Del Negro, G.M.B.; Giudice, M.C.; Tringoni, M.P.; Thomaz, D.Y.; Motta, A.L.; Abdala, E.; Pierroti, L.C.; et al. Candida haemulonii Complex Species, Brazil, January 2010–March 2015. Emerg. Infect. Dis. 2016, 22, 561–563. [Google Scholar] [CrossRef]
  11. Kumar, A.; Prakash, A.; Singh, A.; Kumar, H.; Hagen, F.; Meis, J.F.; Chowdhary, A. Candida haemulonii species complex: An emerging species in India and its genetic diversity assessed with multilocus sequence and amplified fragment-length polymorphism analyses. Emerg. Microbes Infect. 2016, 5, 49. [Google Scholar] [CrossRef] [PubMed]
  12. Hou, X.; Xiao, M.; Chen, S.C.; Wang, H.; Cheng, J.-W.; Chen, X.-X.; Xu, Z.-P.; Fan, X.; Kong, F.; Xu, Y.-C. Identification and Antifungal Susceptibility Profiles of Candida haemulonii Species Complex Clinical Isolates from a Multicenter Study in China. J. Clin. Microbiol. 2016, 54, 2676–2680. [Google Scholar] [CrossRef]
  13. Chen, X.F.; Zhang, H.; Jia, X.M.; Cao, J.; Li, L.; Hu, X.-L.; Li, N.; Xiao, Y.-L.; Xia, F.; Ye, L.Y.; et al. Antifungal susceptibility profiles and drug resistance mechanisms of clinical Candida duobushaemulonii isolates from China. Front. Microbiol. 2022, 13, 1001845. [Google Scholar] [CrossRef] [PubMed]
  14. Tsai, M.H.; Hsu, J.F.; Yang, L.Y.; Pan, Y.B.; Lai, M.Y.; Chu, S.M.; Huang, H.R.; Chiang, M.C.; Fu, R.H.; Lu, J.J. Candidemia due to uncommon Candida species in children: New threat and impacts on outcomes. Sci. Rep. 2018, 8, 15239. [Google Scholar] [CrossRef]
  15. Ramos, L.S.; Branquinha, M.H.; Santos, A.L. Different classes of hydrolytic enzymes produced by multidrug-resistant yeasts comprising the Candida haemulonii complex. Med. Mycol. 2017, 55, 228–232. [Google Scholar] [CrossRef]
  16. Zhou, H.; Yang, X.; Zhou, Q.; Hu, C.; Xin, C.; Song, Z. Character Virulence Association Factors and Gene Mutation Mediating Multidrug Resistance Phenotypes in Candidozyma duobushaemulonii. Mycopathologia 2025, 190, 95. [Google Scholar] [CrossRef]
  17. Araúz, A.B.; Caceres, D.H.; Santiago, E.; Armstrong, P.; Arosemena, S.; Ramos, C.; Espinosa-Bode, A.; Borace, J.; Hayer, L.; Cedeño, I.; et al. Isolation of Candida auris from 9 patients in Central America: Importance of accurate diagnosis and susceptibility testing. Mycoses 2018, 61, 44–47. [Google Scholar] [CrossRef]
  18. Teixeira De Macedo, A.; Wagner de Castro Lima Santos, D.; Spruijtenburg, B.; de Souza, D.A.C.; Dos Santos Barbosa, L.F.M.; Marques, S.; Dos Santos, J.R.A.; Meijer, E.F.J.; de Groot, T.; de Azevedo, C.M.P.E.S.; et al. Clonal outbreak of Candida vulturna in a paediatric oncology ward in Maranhão, Brazil. J. Infect. 2024, 89, 106349. [Google Scholar] [CrossRef]
  19. Jurado-Martín, I.; Marcos-Arias, C.; Tamayo, E.; Guridi, A.; de Groot, P.W.J.; Quindós, G.; Eraso, E. Candida duobushaemulonii: An old but unreported pathogen. J. Fungi 2020, 6, 374. [Google Scholar] [CrossRef]
  20. Fang, S.-Y.; Wei, K.-C.; Chen, W.-C.; Lee, S.J.; Yang, K.C.; Wu, C.S.; Sun, P.L. Primary deep cutaneous candidiasis caused by Candida duobushaemulonii in a 68-year-old man: The first case report and literature review. Mycoses 2016, 59, 818–821. [Google Scholar] [CrossRef] [PubMed]
  21. Boatto, H.F.; Cavalcanti, S.D.; Del Negro, G.M.; Girão, M.J.; Francisco, E.C.; Ishida, K.; Gompertz, O.F. Candida duobushaemulonii: An emerging rare pathogenic yeast isolated from recurrent vulvovaginal candidiasis in Brazil. Mem. Inst. Oswaldo Cruz 2016, 111, 407–410. [Google Scholar] [CrossRef]
  22. Frías-De-León, M.G.; Martínez-Herrera, E.; Acosta-Altamirano, G.; Arenas, R.; Rodríguez-Cerdeira, C. Superficial candidosis by Candida duobushaemulonii: An emerging microorganism. Infect. Genet. Evol. 2019, 75, 103960. [Google Scholar] [CrossRef]
  23. Grenfell, R.C.; da Silva Junior, A.R.; Del Negro, G.M.; Munhoz, R.B.; Gimenes, V.M.; Assis, D.M.; Rockstroh, A.C.; Motta, A.L.; Rossi, F.; Juliano, L.; et al. Identification of Candida haemulonii Complex Species: Use of ClinProTools(TM) to Overcome Limitations of the Bruker Biotyper(TM), VITEK MS(TM) IVD, and VITEK MS(TM) RUO Databases. Front. Microbiol. 2016, 7, 940. [Google Scholar] [CrossRef]
  24. Francisco, E.C.; de Jong, A.W.; Colombo, A.L. Candida haemulonii Species Complex: A Mini-review. Mycopathologia 2023, 188, 909–917. [Google Scholar] [CrossRef]
  25. Lima, S.L.; Francisco, E.C.; De Almeida Júnior, J.N.; Santos, D.W.C.L.; Carlesse, F.; Queiroz-Telles, F.; Melo, A.S.A.; Colombo, A.L. Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex Among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years. J. Fungi 2020, 6, 110. [Google Scholar] [CrossRef]
  26. Buonafine, C.P.; dos Santos, A.E.; Favarello, L.M.; Cognialli, R.C.R.; Aquino, V.R.; Mendes, A.V.A.; Guimarães, T.; Sukiennik, T.C.T.; Carlesse, F.; de Tarso, O.E.; et al. Prevalence rates, species distribution, and antifungal susceptibility of rare Candida and related Saccharomycotina yeasts causing bloodstream infections in 28 medical centers. Mycoses 2026, 69. [Google Scholar] [CrossRef]
  27. Ramos, L.S.; Figueiredo-Carvalho, M.H.; Barbedo, L.S.; Ziccardi, M.; Chaves, A.L.; Zancope-Oliveira, R.M.; Pinto, M.R.; Sgarbi, D.B.; Dornelas-Ribeiro, M.; Branquinha, M.H.; et al. Candida haemulonii Complex: Species Identification and Antifungal Susceptibility Profiles of Clinical Isolates from Brazil. J. Antimicrob. Chemother. 2015, 70, 111–115. [Google Scholar] [CrossRef] [PubMed]
  28. Ramos, L.S.; Figueiredo-Carvalho, M.H.G.; Silva, L.N.; Siqueira, N.L.M.; Lima, J.C.; Oliveira, S.S.; Almeida-Paes, R.; Zancopé Oliveira, R.M.; Azevedo, F.S.; Ferreira, A.L.P.; et al. The Threat Called Candida haemulonii Species Complex in Rio de Janeiro State, Brazil: Focus on Antifungal Resistance and Virulence Attributes. J. Fungi 2022, 8, 574. [Google Scholar] [CrossRef] [PubMed]
  29. Nobrega de Almeida, J.; Brandão, I.B.; Francisco, E.C.; de Almeida, S.L.R.; de Oliveira Dias, P.; Pereira, F.M.; Santos Ferreira, F.; de Andrade, T.S.; de Miranda Costa, M.M.; de Souza Jordão, R.T.; et al. Axillary Digital Thermometers Uplifted a Multidrug Susceptible Candida auris Outbreak among COVID-19 Patients in Brazil. Mycoses 2021, 64, 1062–1072. [Google Scholar] [CrossRef]
  30. Spruijtenburg, B.; Nobrega de Almeida Júnior, J.; Ribeiro, F.d.C.; Kemmerich, K.K.; Baeta, K.; Meijer, E.F.J.; de Groot, T.; Meis, J.F.; Colombo, A.L. Multicenter Candida auris Outbreak Caused by Azole-Susceptible Clade IV in Pernambuco, Brazil. Mycoses 2024, 67, e13752. [Google Scholar] [CrossRef]
  31. Suphavilai, C.; Ko, K.K.K.; Lim, K.M.; Tan, M.G.; Boonsimma, P.; Chu, J.J.K.; Goh, S.S.; Rajandran, P.; Lee, L.C.; Tan, K.Y.; et al. Detection and characterisation of a sixth Candida auris clade in Singapore: A genomic and phenotypic study. Lancet Microbe 2024, 5, 100878. [Google Scholar] [CrossRef]
  32. Xie, O.; Streitberg, R.; Hughes, C.; Stuart, R.; Graham, M. Candida duobushaemulonii sepsis and Candida auris co-isolation following hospitalisation in Vietnam. Pathology 2020, 52, 590–591. [Google Scholar] [CrossRef] [PubMed]
  33. Goyer, M.; Lucchi, G.; Ducoroy, P.; Vagner, O.; Bonnin, A.; Dalle, F. Optimization of the Preanalytical Steps of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Identification Provides a Flexible and Efficient Tool for Identification of Clinical Yeast Isolates in Medical Laboratories. J. Clin. Microbiol. 2012, 50, 3066–3068. [Google Scholar] [CrossRef] [PubMed]
  34. CLSI standard M27; Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. 4th ed. Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2017.
  35. Spruijtenburg, B.; Jacomel Favoreto de Souza Lima, B.; Granadillo Tosar, T.; Borman, A.M.; Andersen, C.T.; Nizamuddin, S.; Ahmad, S.; de Almeida Junior, J.N.; Vicente, V.A.; Nosanchuk, J.D.; et al. The yeast genus Tardiomyces gen. nov. with one new species and two new combinations. Infection 2024, 52, 1799–1812. [Google Scholar] [CrossRef]
  36. de Groot, T.; Spruijtenburg, B.; Parnell, L.A.; Chow, N.A.; Meis, J.F. Optimization and Validation of Candida auris Short Tandem Repeat Analysis. Microbiol. Spectr. 2022, 10, e0264522. [Google Scholar] [CrossRef]
Antibiotics 15 00237 g001
Figure 1. Phylogenetic tree based on the ITS region of Candida haemulonii species complex including Clavispora lusitaniae as outgroup. Isolates from the current study are marked in bold.
Figure 1. Phylogenetic tree based on the ITS region of Candida haemulonii species complex including Clavispora lusitaniae as outgroup. Isolates from the current study are marked in bold.
Antibiotics 15 00237 g001
Antibiotics 15 00237 g002
Figure 2. Whole genome sequencing (WGS) single nucleotide polymorphism (SNP) analysis of C. duobushaemulonii isolates. On 33 publicly available WGS data, SNP analysis was performed. Isolates from this study (2022–2024) are marked in bold. B09383 was used as the reference genome, and positions with a minimal coverage of 15 were included for the comparative analysis and the tree was constructed with the neighbor-joining method using MEGA11 (v11.0.10) and iTOL (v6) software.
Figure 2. Whole genome sequencing (WGS) single nucleotide polymorphism (SNP) analysis of C. duobushaemulonii isolates. On 33 publicly available WGS data, SNP analysis was performed. Isolates from this study (2022–2024) are marked in bold. B09383 was used as the reference genome, and positions with a minimal coverage of 15 were included for the comparative analysis and the tree was constructed with the neighbor-joining method using MEGA11 (v11.0.10) and iTOL (v6) software.
Antibiotics 15 00237 g002
Table 1. Clinical characteristics and factors associated with invasive Candida duobushaemulonii infection in a pediatric ward.
Table 1. Clinical characteristics and factors associated with invasive Candida duobushaemulonii infection in a pediatric ward.
PatientDate of FungemiaAge/GenderCancer DiagnosisCentral Vascular Catheter-DaysIndwelling Urethral Catheter-DaysNasoenteric TubeParenteral Nutrition-DaysFever (>37.8C)ChillsHypotension (Mean Arterial Pressure ≤ 90 mmHg)Tachycardia (>100 bpm)
CD2004/25/227/MALL-B *PICC ***
30 days		NoNoNoYesYesYesNo
CD2203/27/224/FALL-B *PICC ***
32 days		NoNoNoYesYesYesYes
CD2304/04/2210/FALL-B *PICC ***
62 days		NoNoNoNoYesNoNo
CD2502/14/2212/MALL-B *PICC ***
69 days		NoNoYes/13 dYesNoNoYes
66999228805/07/244/MRetroperitoneal embryonic rhabdomyosarcoma (lung as primary focus)DL-CVC ****
14 days		Yes/90 dYes/30 dYes/20 dYesYesNoYes
66967141006/08/243/FALL-T **PICC ***
21 days		NoNoNoYesYesYesYes
* Acute lymphoblastic leukemia of B cells, ** Acute lymphoblastic leukemia of T cells, *** Peripherally inserted central catheter, **** Double-lumen central venous catheter.
Table 2. Laboratory data, treatment and outcome of invasive Candida duobushaemulonii infection in a pediatric ward.
Table 2. Laboratory data, treatment and outcome of invasive Candida duobushaemulonii infection in a pediatric ward.
PatientNeutrophils/mm3 *Kidney Dysfunction *Concomitant Bacteremia *Echocardiography *Antifungal TherapyPersistent Candidemia30 Day-Outcome
CD201476NoNoNoFLU **Nodeath
CD2231NoS.epidermidisYes-No vegetationFLUNoalive
CD231071NoNoNoFLUNoalive
CD2530NoNoYes-No vegetationFLUNodeath
66999228820,939NoAcinetobacter baumanniiYes-No vegetationFCZ-switched to MFG ***Nodeath
669671410411NoNoNoFLU-switched to AMB ****Nodeath
* at the time of fungemia, ** Fluconazole, *** Micafungin, **** Amphotericin B.
Table 3. Minimum inhibitory concentrations (MICs) of six Candida duobushaemulonii isolates from two outbreaks against eight antifungals according to Clinical and Laboratory Standards Institute (CLSI) M27 guideline.
Table 3. Minimum inhibitory concentrations (MICs) of six Candida duobushaemulonii isolates from two outbreaks against eight antifungals according to Clinical and Laboratory Standards Institute (CLSI) M27 guideline.
Lab IDAMBFLUITCVORPOSISAAFGMFG
CD20240.0630.0630.0310.0310.0630.063
CD22240.0630.063≤0.016≤0.0160.1250.063
CP23240.0630.063≤0.016≤0.0160.0630.063
CD25240.0630.0630.0310.0310.0630.063
669992288280.0630.0630.0310.0310.0630.125
669671410240.1250.125≤0.0160.0310.1250.063
AMB, amphotericin B; FLU, fluconazole; ITC, itraconazole; VOR, voriconazole; POS, posaconazole; ISA, isavuconazole; AFG, anidulafungin; MFG, micafungin. All MICs are in µg/mL.
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Wagner de Castro Lima Santos, D.; Spruijtenburg, B.; Meijer, E.F.J.; Azevedo Coelho de Souza, D.; de Maria Pedrozo e Silva de Azevedo, C.; Meis, J.F. Silent Outbreaks of Candida duobushaemulonii in a Pediatric Ward in Brazil. Antibiotics 2026, 15, 237. https://doi.org/10.3390/antibiotics15030237

AMA Style

Wagner de Castro Lima Santos D, Spruijtenburg B, Meijer EFJ, Azevedo Coelho de Souza D, de Maria Pedrozo e Silva de Azevedo C, Meis JF. Silent Outbreaks of Candida duobushaemulonii in a Pediatric Ward in Brazil. Antibiotics. 2026; 15(3):237. https://doi.org/10.3390/antibiotics15030237

Chicago/Turabian Style

Wagner de Castro Lima Santos, Daniel, Bram Spruijtenburg, Eelco F. J. Meijer, Dayse Azevedo Coelho de Souza, Conceição de Maria Pedrozo e Silva de Azevedo, and Jacques F. Meis. 2026. "Silent Outbreaks of Candida duobushaemulonii in a Pediatric Ward in Brazil" Antibiotics 15, no. 3: 237. https://doi.org/10.3390/antibiotics15030237

APA Style

Wagner de Castro Lima Santos, D., Spruijtenburg, B., Meijer, E. F. J., Azevedo Coelho de Souza, D., de Maria Pedrozo e Silva de Azevedo, C., & Meis, J. F. (2026). Silent Outbreaks of Candida duobushaemulonii in a Pediatric Ward in Brazil. Antibiotics, 15(3), 237. https://doi.org/10.3390/antibiotics15030237

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