Development of a Galleria mellonella Infection Model to Evaluate the Efficacy of Antibiotic-Loaded Polymethyl Methacrylate (PMMA) Bone Cement
Abstract
:1. Introduction
2. Results
2.1. ALBC Discs Show Released Activity and Inhibit Bacterial Attachment In Vitro
2.2. ALBC Implants Prevent S. aureus Infections In Vivo
2.2.1. ALBC Is Biocompatible in G. mellonella Larvae
2.2.2. ALBC Implants Prevent Both Early-Stage Biofilm and Hematogenous Infections In Vivo
2.2.3. ALBC Implants Eradicate Bacteria on Surface and in Tissue
3. Discussion
4. Materials and Methods
4.1. Bacterial Cultures
4.2. Preparation of ALBC Discs and Implants
4.3. In Vitro Antimicrobial Activity of ALBC Discs
4.4. G. mellonella Implant Infection Models
4.4.1. Animals
4.4.2. Early-Stage Biofilm Implant Infection Model
4.4.3. Hematogenous Implant Infection Model
4.4.4. Quantitative Culture
4.5. Scanning Electron Microscopy
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhao, Y.; Mannala, G.K.; Youf, R.; Rupp, M.; Alt, V.; Riool, M. Development of a Galleria mellonella Infection Model to Evaluate the Efficacy of Antibiotic-Loaded Polymethyl Methacrylate (PMMA) Bone Cement. Antibiotics 2024, 13, 692. https://doi.org/10.3390/antibiotics13080692
Zhao Y, Mannala GK, Youf R, Rupp M, Alt V, Riool M. Development of a Galleria mellonella Infection Model to Evaluate the Efficacy of Antibiotic-Loaded Polymethyl Methacrylate (PMMA) Bone Cement. Antibiotics. 2024; 13(8):692. https://doi.org/10.3390/antibiotics13080692
Chicago/Turabian StyleZhao, You, Gopala Krishna Mannala, Raphaëlle Youf, Markus Rupp, Volker Alt, and Martijn Riool. 2024. "Development of a Galleria mellonella Infection Model to Evaluate the Efficacy of Antibiotic-Loaded Polymethyl Methacrylate (PMMA) Bone Cement" Antibiotics 13, no. 8: 692. https://doi.org/10.3390/antibiotics13080692
APA StyleZhao, Y., Mannala, G. K., Youf, R., Rupp, M., Alt, V., & Riool, M. (2024). Development of a Galleria mellonella Infection Model to Evaluate the Efficacy of Antibiotic-Loaded Polymethyl Methacrylate (PMMA) Bone Cement. Antibiotics, 13(8), 692. https://doi.org/10.3390/antibiotics13080692