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Volume 13
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10.3390/antibiotics13030210
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Article

Anti-Listerial Activity of Bacteriocin-like Inhibitory Substance Produced by Enterococcus lactis LBM BT2 Using Alternative Medium with Sugarcane Molasses

by
Taciana Freire de Oliveira
1,†,
Taís Mayumi Kuniyoshi
1,†,
Elionio Galvão Frota
1,
Sebastián Bermúdez-Puga
1,
Letícia Naomy Sakaue
1,
Luara Lucena Cassiano
2,
Leonardo Tachibana
2,
Rosane Aparecida Moniz Piccoli
3,
Attilio Converti
4 and
Ricardo Pinheiro de Souza Oliveira
1,*
1
Laboratory of Microbial Biomolecules, School of Pharmaceutical Sciences, University of São Paulo, Rua do Lago, 250, São Paulo 05508-000, Brazil
2
Aquaculture Research Center, Scientific Research of Fisheries Institute, APTA, SAA, Av. Conselheiro Rodrigues Alves, 1252, São Paulo 04014-002, Brazil
3
Bionanomanufacturing Nucleus, Institute for Technological Research (IPT), Av. Prof. Almeida Prado, 532, São Paulo 05508-901, Brazil
4
Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Antibiotics 2024, 13(3), 210; https://doi.org/10.3390/antibiotics13030210
Submission received: 30 January 2024 / Revised: 17 February 2024 / Accepted: 20 February 2024 / Published: 23 February 2024
(This article belongs to the Special Issue Updates on Major Food-Related Pathogens: Antimicrobial Treatment, Drug Resistance, Inactivation, and Related Diseases)
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Abstract

Listeria monocytogenes is a foodborne pathogen that contaminates food-processing environments and persists within biofilms on equipment, thus reaching final products by cross-contamination. With the growing demand for clean-label products, the search for natural antimicrobials as biopreservants, such as bacteriocins, has shown promising potential. In this context, this study aimed to evaluate the anti-listerial action of bacteriocins produced by Enterococcus lactis LBM BT2 in an alternative medium containing sugarcane molasses (SCM). Molecular analyses were carried out to characterize the strain, including the presence of bacteriocin-related genes. In the kinetic study on SCM medium E. lactis, LBM BT2 showed biomass and bacteriocin productions similar to those observed on a sucrose-based medium (control), highlighting the potential of the sugarcane molasses as a low-cost substrate. Stability tests revealed that the molecule remained active in wide ranges of pH (4–10) and temperature (60–100 °C). Furthermore, the proteolytic treatment reduced the biomolecule’s antimicrobial activity, highlighting its proteinaceous nature. After primary purification by salting out and tangential flow filtration, the bacteriocin-like inhibitory substance (BLIS) showed bacteriostatic activity on suspended L. monocytogenes cells and against biofilm formation at a concentration of 0.625 mg/mL. These results demonstrate the potential of the produced BLIS as a biopreservative in the food industry.
Keywords: bacteriocin-like inhibitory substance; Enterococcus lactis; Listeria monocytogenes; anti-listerial activity; sugarcane molasses bacteriocin-like inhibitory substance; Enterococcus lactis; Listeria monocytogenes; anti-listerial activity; sugarcane molasses

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MDPI and ACS Style

Oliveira, T.F.d.; Kuniyoshi, T.M.; Frota, E.G.; Bermúdez-Puga, S.; Sakaue, L.N.; Cassiano, L.L.; Tachibana, L.; Piccoli, R.A.M.; Converti, A.; Oliveira, R.P.d.S. Anti-Listerial Activity of Bacteriocin-like Inhibitory Substance Produced by Enterococcus lactis LBM BT2 Using Alternative Medium with Sugarcane Molasses. Antibiotics 2024, 13, 210. https://doi.org/10.3390/antibiotics13030210

AMA Style

Oliveira TFd, Kuniyoshi TM, Frota EG, Bermúdez-Puga S, Sakaue LN, Cassiano LL, Tachibana L, Piccoli RAM, Converti A, Oliveira RPdS. Anti-Listerial Activity of Bacteriocin-like Inhibitory Substance Produced by Enterococcus lactis LBM BT2 Using Alternative Medium with Sugarcane Molasses. Antibiotics. 2024; 13(3):210. https://doi.org/10.3390/antibiotics13030210

Chicago/Turabian Style

Oliveira, Taciana Freire de, Taís Mayumi Kuniyoshi, Elionio Galvão Frota, Sebastián Bermúdez-Puga, Letícia Naomy Sakaue, Luara Lucena Cassiano, Leonardo Tachibana, Rosane Aparecida Moniz Piccoli, Attilio Converti, and Ricardo Pinheiro de Souza Oliveira. 2024. "Anti-Listerial Activity of Bacteriocin-like Inhibitory Substance Produced by Enterococcus lactis LBM BT2 Using Alternative Medium with Sugarcane Molasses" Antibiotics 13, no. 3: 210. https://doi.org/10.3390/antibiotics13030210

APA Style

Oliveira, T. F. d., Kuniyoshi, T. M., Frota, E. G., Bermúdez-Puga, S., Sakaue, L. N., Cassiano, L. L., Tachibana, L., Piccoli, R. A. M., Converti, A., & Oliveira, R. P. d. S. (2024). Anti-Listerial Activity of Bacteriocin-like Inhibitory Substance Produced by Enterococcus lactis LBM BT2 Using Alternative Medium with Sugarcane Molasses. Antibiotics, 13(3), 210. https://doi.org/10.3390/antibiotics13030210

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