Genome-Based Retrospective Analysis of a Providencia stuartii Outbreak in Rome, Italy: Broad Spectrum IncC Plasmids Spread the NDM Carbapenemase within the Hospital
Abstract
:1. Introduction
2. Results and Discussion
2.1. An Outbreak of NDM-Producing Providencia Stuartii
2.2. The NDM-Carrying IncC Plasmid
- p0831_NDM_IncC carried an Insertion Sequence (IS, ISEc23), which was not present in the P. stuartii plasmid;
- pVCpro_41 carried three genes, which were not present in the p0831_NDM_IncC plasmid, encoding for an uncharacterized MFS-type transporter, and two uncharacterized proteins named YjiL and YjiM.
2.3. Genomic Epidemiology of blaNDM-Carrying IncC Plasmids
2.4. Anatomy of Three Providencia Stuartii Isolates Carrying the IncC Plasmid
3. Materials and Methods
3.1. Bacterial Isolation and Antimicrobial Susceptibility Testing
3.2. Whole-Genome Sequencing and Assembly
3.3. Genomic and Phylogenetic Analyses
3.4. IncC Plasmids Analysis
3.5. Statistical Analyses
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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PT | ID | SS | MIC mg/L | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
AK | P/T | CAZ | FOX | CRM | CZA | C/T | IMI | MEM | CIP | SXT | AZT | FOS | |||
Pt#1 | 41 * | RS | 32 | >16 | >32 | >16 | >8 | >16 | >32 | >16 | >16 | >4 | 80 | ≤1 | >64 |
Pt#2 | 38 | RS | 32 | >16 | >32 | >16 | >8 | >16 | >32 | >16 | >16 | >4 | 40 | 2 | >64 |
Pt#3 | 883 * | TBA | 32 | >16 | >32 | >16 | >8 | >16 | >32 | >16 | >16 | >4 | 40 | ≤1 | >64 |
919 | UR | 32 | >16 | >32 | >16 | >8 | >16 | >32 | >16 | >16 | >4 | 40 | ≤1 | >64 | |
Pt#4 | 65 * | UR | 32 | >16 | >32 | >16 | >8 | >16 | >32 | >16 | >16 | >4 | 40 | ≤1 | >64 |
607 | TBA | 32 | >16 | >32 | >16 | >8 | >16 | >32 | >16 | >16 | >4 | 40 | 2 | >64 | |
317 | TBA | 32 | >16 | >32 | >16 | >8 | >16 | >32 | >16 | >16 | >4 | 40 | ≤1 | >64 |
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Capitani, V.; Arcari, G.; Oliva, A.; Sacco, F.; Menichincheri, G.; Fenske, L.; Polani, R.; Raponi, G.; Antonelli, G.; Carattoli, A. Genome-Based Retrospective Analysis of a Providencia stuartii Outbreak in Rome, Italy: Broad Spectrum IncC Plasmids Spread the NDM Carbapenemase within the Hospital. Antibiotics 2023, 12, 943. https://doi.org/10.3390/antibiotics12050943
Capitani V, Arcari G, Oliva A, Sacco F, Menichincheri G, Fenske L, Polani R, Raponi G, Antonelli G, Carattoli A. Genome-Based Retrospective Analysis of a Providencia stuartii Outbreak in Rome, Italy: Broad Spectrum IncC Plasmids Spread the NDM Carbapenemase within the Hospital. Antibiotics. 2023; 12(5):943. https://doi.org/10.3390/antibiotics12050943
Chicago/Turabian StyleCapitani, Valerio, Gabriele Arcari, Alessandra Oliva, Federica Sacco, Gaia Menichincheri, Linda Fenske, Riccardo Polani, Giammarco Raponi, Guido Antonelli, and Alessandra Carattoli. 2023. "Genome-Based Retrospective Analysis of a Providencia stuartii Outbreak in Rome, Italy: Broad Spectrum IncC Plasmids Spread the NDM Carbapenemase within the Hospital" Antibiotics 12, no. 5: 943. https://doi.org/10.3390/antibiotics12050943