Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model
Abstract
:1. Introduction
2. Results
2.1. Chemical Analysis by GC-MS of the Essential Oils of M. spicata and E. globulus
2.2. Antibacterial Activity of the Essential Oil of M. spicata and E. globulus against S. mutans
2.3. Antibiofilm Activity Using an In Vitro Model on Dental Enamel Pieces of Bovine Origin
2.3.1. Culture Medium pH Fluctuation
2.3.2. Spectrophotometric Turbidity
2.3.3. Recoverable Microorganism Count
3. Discussion
4. Materials and Methods
4.1. Obtention of the Essential Oil
4.2. Composition Analysis by Gas Chromatography-Mass Spectrometry (GC/MS) of Essential Oils
4.3. Inhibitory Activity of the Essential Oils M. spicata and E. globulus and Determination of the Minimum Inhibitory Concentration
4.3.1. Evaluation of the Ability to Form Biofilms by the Congo Red Method
4.3.2. Agar-Well Diffusion Method
4.3.3. Determination of the Minimum Inhibitory Concentration (MIC) by the Colorimetric Microdilution Method
4.4. In Vitro Model for the Formation of Biofilms on Dental Enamel Pieces of Bovine Origin
4.4.1. Culture Media
4.4.2. Activation of the Microorganism and Preparation of the Inoculum
4.4.3. Preparation of Enamel Slabs
4.4.4. Biofilm Cultivation and Treatment
4.4.5. Biofilm Collection and Evaluation of the Treatment
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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N | Compound | Retention Index | % of Relative Area |
---|---|---|---|
1 | α-Pinene | 936 | 3.77 |
2 | Sabinene | 956 | 1.16 |
3 | α-Myrcene | 985 | 2.00 |
5 | 3-Carene | 1010 | 3.70 |
6 | Limonene | 1020 | 12.91 |
7 | 1,8-Cineol (eucalyptol) | 1033 | 1.20 |
8 | γ-Terpinene | 1062 | 0.33 |
9 | cis-α-Terpineol | 1078 | 0.20 |
10 | Terpinolene | 1086 | 0.41 |
11 | p-Menthan-3-one | 1092 | 0.70 |
12 | Menthone | 1125 | 0.37 |
13 | 1-menthol | 1136 | 1.88 |
14 | Carvone | 1142 | 57.93 |
15 | 2-isopropyl-5-methyl-3-cyclohexen-1-one | 1156 | 0.20 |
16 | p-Menthane | 1263 | 5.88 |
17 | Copaene | 1376 | 1.44 |
18 | α-Bourbonene | 1385 | 0.61 |
19 | Caryophyllene | 1420 | 1.03 |
N | Compound. | Retention Index | % of Relative Areas |
---|---|---|---|
1 | α-Pinene | 936 | 18.15 |
2 | β-Pinene | 983 | 1.78 |
3 | α–Myrcene | 985 | 1.56 |
4 | 1,8-Cineol (eucalyptol) | 1033 | 65.83 |
5 | 4-Terpineol | 1182 | 0.77 |
6 | α-Terpinol | 1242 | 2.02 |
7 | α-Terpineol acetate | 1330 | 5.14 |
8 | (+)-Aromadendrene | 1440 | 2.84 |
9 | Globulol | 1590 | 1.92 |
Essential Oil | Concentration | Diameter ± SD (mm) |
---|---|---|
Mentha spicata | 100% | 18.30 ± 0.47 |
50% | 15.75 ± 0.41 | |
10% | 13.66 ± 0.47 | |
Eucalyptus globulus | 100% | 27.00 ± 0.82 |
50% | 18.00 ± 0.82 | |
10% | 12.00 ± 0.82 | |
Solvent (DMSO) | 0.1% | 0 |
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Landeo-Villanueva, G.E.; Salazar-Salvatierra, M.E.; Ruiz-Quiroz, J.R.; Zuta-Arriola, N.; Jarama-Soto, B.; Herrera-Calderon, O.; Pari-Olarte, J.B.; Loyola-Gonzales, E. Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model. Antibiotics 2023, 12, 369. https://doi.org/10.3390/antibiotics12020369
Landeo-Villanueva GE, Salazar-Salvatierra ME, Ruiz-Quiroz JR, Zuta-Arriola N, Jarama-Soto B, Herrera-Calderon O, Pari-Olarte JB, Loyola-Gonzales E. Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model. Antibiotics. 2023; 12(2):369. https://doi.org/10.3390/antibiotics12020369
Chicago/Turabian StyleLandeo-Villanueva, Guillermo Ernesto, María Elena Salazar-Salvatierra, Julio Reynaldo Ruiz-Quiroz, Noemi Zuta-Arriola, Benjamín Jarama-Soto, Oscar Herrera-Calderon, Josefa Bertha Pari-Olarte, and Eddie Loyola-Gonzales. 2023. "Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model" Antibiotics 12, no. 2: 369. https://doi.org/10.3390/antibiotics12020369