Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of Pseudomonas aeruginosa

Kim, Young-Min; Son, Hyosuk; Park, Seong-Cheol; Lee, Jong-Kook; Jang, Mi-Kyeong; Lee, Jung Ro

doi:10.3390/antibiotics12020349

Open AccessArticle

Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of Pseudomonas aeruginosa

by

Young-Min Kim

^1,†,

Hyosuk Son

^1,2,†,

Seong-Cheol Park

^1,†,

Jong-Kook Lee

¹,

Mi-Kyeong Jang

^1,* and

Jung Ro Lee

^3,*

¹

Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of Korea

²

Department of Exhibition and Education, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea

³

LMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of Korea

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Antibiotics 2023, 12(2), 349; https://doi.org/10.3390/antibiotics12020349

Submission received: 14 January 2023 / Revised: 5 February 2023 / Accepted: 6 February 2023 / Published: 8 February 2023

(This article belongs to the Special Issue Action Mechanism of Peptide Variants with Antimicrobial, Anti-Biofilm, or Anti-Inflammatory Effects)

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Abstract

Biofilms are resistant to antibiotics and are a major source of persistent and recurring infections by clinically important pathogens. Drugs used for biofilm-associated infections are limited because biofilm-embedded or biofilm-matrix bacteria are difficult to kill or eradiate. Therefore, many researchers are developing new and effective antibiofilm agents. Among them, antimicrobial peptides have an attractive interest in the development of antibiofilm agents. The present study evaluated the effects of 10 synthetic peptides on growth inhibition, inhibition of biofilm formation, and biofilm elimination in drug-resistant Pseudomonas aeruginosa. The planktonic cell growth and biofilm formation were dose-dependently inhibited by most of the peptides. WIK-14 eliminated preformed biofilm masses by removing carbohydrates, extracellular nucleic acids, proteins, and lipids constituting extracellular polymeric substances. The results demonstrated that WIK-14 and WIKE-14 peptides might provide novel therapeutic drugs to overcome multidrug resistance in biofilm-associated infections.

Keywords: Pseudomonas aeruginosa; planktonic cells; antimicrobial peptide; antimicrobial resistance; biofilm

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MDPI and ACS Style

Kim, Y.-M.; Son, H.; Park, S.-C.; Lee, J.-K.; Jang, M.-K.; Lee, J.R. Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of Pseudomonas aeruginosa. Antibiotics 2023, 12, 349. https://doi.org/10.3390/antibiotics12020349

AMA Style

Kim Y-M, Son H, Park S-C, Lee J-K, Jang M-K, Lee JR. Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of Pseudomonas aeruginosa. Antibiotics. 2023; 12(2):349. https://doi.org/10.3390/antibiotics12020349

Chicago/Turabian Style

Kim, Young-Min, Hyosuk Son, Seong-Cheol Park, Jong-Kook Lee, Mi-Kyeong Jang, and Jung Ro Lee. 2023. "Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of Pseudomonas aeruginosa" Antibiotics 12, no. 2: 349. https://doi.org/10.3390/antibiotics12020349

APA Style

Kim, Y.-M., Son, H., Park, S.-C., Lee, J.-K., Jang, M.-K., & Lee, J. R. (2023). Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of Pseudomonas aeruginosa. Antibiotics, 12(2), 349. https://doi.org/10.3390/antibiotics12020349

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of Pseudomonas aeruginosa

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