Bioengineering the Antimicrobial Activity of Yeast by Recombinant Thanatin Production
Abstract
:1. Introduction
2. Results
2.1. Bioengineering rThan—Producing Yeast
2.2. Constitutive Production and Purification of rThan
2.3. Chemical Synthesis of Thanatin
2.4. Antimicrobial Activity Spectra of rThan
2.5. Cocultivation Experiments Reveal Biocontrol Potential of rThan-Producing Yeasts
3. Discussion
4. Materials and Methods
4.1. Bacterial and Yeast Strains
4.2. Expression Vector Design and Construction
4.3. Yeast Transformation, rThan Production and Purification
4.4. Agar Overlay Assay
4.5. Cocultivation with Target Bacteria
4.6. Peptide Chemical Synthesis
4.6.1. Materials
4.6.2. Peptide Synthesis
4.6.3. Disulfide Bond Formation in Thanatin
4.6.4. Peptide Purification
4.7. Evaluation of Antimicrobial Activity
4.8. Evaluation of Cytotoxicity
4.9. Liquid Chromatography and Mass Spectrometry
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pipiya, S.O.; Kudzhaev, A.M.; Mirzoeva, N.Z.; Mokrushina, Y.A.; Ziganshin, R.H.; Komlev, A.S.; Petrova, P.E.; Smirnov, I.V.; Gabibov, A.G.; Shamova, O.V.; et al. Bioengineering the Antimicrobial Activity of Yeast by Recombinant Thanatin Production. Antibiotics 2023, 12, 1719. https://doi.org/10.3390/antibiotics12121719
Pipiya SO, Kudzhaev AM, Mirzoeva NZ, Mokrushina YA, Ziganshin RH, Komlev AS, Petrova PE, Smirnov IV, Gabibov AG, Shamova OV, et al. Bioengineering the Antimicrobial Activity of Yeast by Recombinant Thanatin Production. Antibiotics. 2023; 12(12):1719. https://doi.org/10.3390/antibiotics12121719
Chicago/Turabian StylePipiya, Sofiya O., Arsen M. Kudzhaev, Nisso Z. Mirzoeva, Yuliana A. Mokrushina, Rustam H. Ziganshin, Alexey S. Komlev, Polina E. Petrova, Ivan V. Smirnov, Alexander G. Gabibov, Olga V. Shamova, and et al. 2023. "Bioengineering the Antimicrobial Activity of Yeast by Recombinant Thanatin Production" Antibiotics 12, no. 12: 1719. https://doi.org/10.3390/antibiotics12121719