Next Article in Journal
Effect of the Symbiosis with Mycoplasma hominis and Candidatus Mycoplasma Girerdii on Trichomonas vaginalis Metronidazole Susceptibility
Previous Article in Journal
Pattern of Antibiotic Use among Hospitalized Patients according to WHO Access, Watch, Reserve (AWaRe) Classification: Findings from a Point Prevalence Survey in Bangladesh
 
 
Article

Clonality and Persistence of Multiresistant Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from the Staff of a University Veterinary Hospital

1
Unidad de Patología Infecciosa y Epidemiología, Facultad de Veterinaria, Universidad de Extremadura, 10003 Cáceres, Spain
2
Área de Producción Animal, CICYTEX-La Orden, 06187 Badajoz, Spain
3
Unidad de Reproducción y Obstetricia, Facultad de Veterinaria, Universidad de Extremadura, 10003 Cáceres, Spain
4
One Health Group, Facultad de Ingeniería y Ciencias Aplicadas (FICA), Biotecnología, Universidad de las Américas (UDLA), Quito 170125, Ecuador
*
Author to whom correspondence should be addressed.
Antibiotics 2022, 11(6), 811; https://doi.org/10.3390/antibiotics11060811
Received: 28 May 2022 / Revised: 13 June 2022 / Accepted: 14 June 2022 / Published: 16 June 2022
The aim of this study was to characterize methicillin-resistant coagulase-negative staphylococci (MRCoNS) isolates from the healthy staff of a university veterinary hospital in order to assess their importance as a reservoir of antimicrobial resistance and to determine their population structure and evolution. The study duration was over two years (2020–2021), 94 individuals were analyzed in duplicate, and 78 strains were obtained. The overall prevalence of methicillin-resistant strains detected throughout the study was 61.7%, with point prevalence values of 53.2% in 2020 and 31.5% in 2021. A total of 19.1% of the individuals analyzed were carriers throughout the study. The most frequently identified MRCoNs were Staphylococcus epidermidis (92.3%) and S. warneri (3.8%). A total of 75.6% of the isolates obtained showed the development of multi-resistance, preferentially against erythromycin, gentamicin, and tetracycline, and to a lesser extent against fusidic acid, norfloxacin, and clindamycin; these antimicrobials are frequently used in the veterinary field. Although most of the S. epidermidis isolates obtained showed wide genetic variability and low dispersion, which are characteristic of community-associated isolates, a small number of strains spread between individuals in close physical proximity and were maintained over time, forming stable clones. These clones generally maintained the same type of staphylococcal cassette chromosome mec (SCCmec) and had a similar antimicrobial resistance pattern. View Full-Text
Keywords: methicillin-resistant; coagulase-negative staphylococci; antimicrobial resistance; multiresistant; clonality; persistence; SCCmec; PFGE methicillin-resistant; coagulase-negative staphylococci; antimicrobial resistance; multiresistant; clonality; persistence; SCCmec; PFGE
Show Figures

Graphical abstract

MDPI and ACS Style

Rey, J.; Gil, M.; de Mendoza, J.H.; García, A.; Gaitskell-Phillips, G.; Bastidas-Caldes, C.; Zalama, L. Clonality and Persistence of Multiresistant Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from the Staff of a University Veterinary Hospital. Antibiotics 2022, 11, 811. https://doi.org/10.3390/antibiotics11060811

AMA Style

Rey J, Gil M, de Mendoza JH, García A, Gaitskell-Phillips G, Bastidas-Caldes C, Zalama L. Clonality and Persistence of Multiresistant Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from the Staff of a University Veterinary Hospital. Antibiotics. 2022; 11(6):811. https://doi.org/10.3390/antibiotics11060811

Chicago/Turabian Style

Rey, Joaquín, María Gil, Javier Hermoso de Mendoza, Alfredo García, Gemma Gaitskell-Phillips, Carlos Bastidas-Caldes, and Laura Zalama. 2022. "Clonality and Persistence of Multiresistant Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from the Staff of a University Veterinary Hospital" Antibiotics 11, no. 6: 811. https://doi.org/10.3390/antibiotics11060811

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop