Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System
Abstract
:1. Introduction
2. Results
2.1. Time Course Changes of Water Quality Parameters in Microalgal Cultures
2.1.1. Total Dissolved Solids
2.1.2. Electric Conductivity
2.1.3. Total Suspended Solids
2.1.4. pH
2.1.5. Temperature
2.1.6. Nitrate Nitrogen
2.2. Occurrence and Relative Abundance of the Genes in the Influent
2.3. The Effect of Treatment on the Relative Abundance of Individual Genes
2.4. Comparison of the Efficiencies of Holding Times in Reduction of Relative Abundances of Total ARGs, Total int1 and Total uidA
2.5. Relationships between ARGs, Indicator Genes and Water Quality Parameters
3. Discussion
3.1. The Effect of Microalgal Cultivation on Water Quality Indicators
3.2. Occurrence and Abundance of ARGs in the MWW
3.3. The Effect of Treatment on ARGs Relative Abundance
3.3.1. β-lactam Resistance Genes: blaCTX and blaTEM
3.3.2. Sulfonamides Resistance Genes: sul1 and sul2
3.3.3. Phenicol Resistance Genes: cmlA and floR
3.3.4. Aminoglycosides Resistance Gene: aadA
3.3.5. Tetracycline Resistance Genes: tetO, tetW and tetX
3.3.6. Macrolide-Lincosamide-Streptogramin Resistance Gene: ermB
3.3.7. The Effect of Treatment on int1 Relative Abundance
3.3.8. The Effect of the Treatment on uidA Relative Abundance
3.4. Comparison of Genes Reduction Performance between Cultures
3.5. Relationships between ARGs, Indicator Genes and Water Quality Parameters
3.5.1. Relationships between ARGs and Indicator Genes
3.5.2. Relationships between ARGs and Water Quality Parameters
4. Materials and Methods
4.1. Outdoor Cultivation of Microalgae Combinations in MWW
4.2. Determination of Physicochemical Water Quality Parameters
4.3. Antibiotic Resistance Genes Pollution Studies
4.3.1. Genomic DNA Extraction
4.3.2. Qualitative Polymerase Chain Reaction
4.3.3. Real-Time Polymerase Chain Reaction
4.4. Statistical Analyses
5. Conclusions, Future Prospects, Limitations and Recommendations
5.1. Conclusions
- Two indicator genes, int1 and uidA, analyzed in this study proved reliable biomarkers for ARGs pollution in the microalgae-based WWT system. Interestingly, the uidA level could be used to monitor potential fecal and/or ARGs pollution. However, the relationship is most precise with aadA, blaCTX, blaTEM and cmlA. In the same vein, int1 proved to be a reliable biomarker for sul1 and aadA genes.
- The abundance of ARGs in the system can be represented indirectly by the concentrations of TDS, EC and TSS. More specifically, TDS and EC related most with sul1, whereas TSS showed the most solid relationship with blaCTX, blaTEM and aadA.
- Tetracycline resistance genes (tetO, tetW and tetX) and ermB were highly vulnerable to reduction in the microalgae-based WWT system, whereas floR was the most persistent.
- Although all the cultures proved promising in removing the conventional MWW quality parameters, the monocultures were the most efficient in terms of gene reduction from the MWW stream. This proves the superiority of the monocultures over the multi-species cultures, synthetic or natural, both in binary or polyculture combinations.
5.2. Future Prospects, Limitations and Recommendations
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Inuwa, A.B.; Mahmood, Q.; Iqbal, J.; Widemann, E.; Shafiq, S.; Irshad, M.; Irshad, U.; Iqbal, A.; Hafeez, F.; Nazir, R. Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System. Antibiotics 2022, 11, 1531. https://doi.org/10.3390/antibiotics11111531
Inuwa AB, Mahmood Q, Iqbal J, Widemann E, Shafiq S, Irshad M, Irshad U, Iqbal A, Hafeez F, Nazir R. Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System. Antibiotics. 2022; 11(11):1531. https://doi.org/10.3390/antibiotics11111531
Chicago/Turabian StyleInuwa, Abdullahi B., Qaisar Mahmood, Jamshed Iqbal, Emilie Widemann, Sarfraz Shafiq, Muhammad Irshad, Usman Irshad, Akhtar Iqbal, Farhan Hafeez, and Rashid Nazir. 2022. "Removal of Antibiotic Resistance Genes, Class 1 Integrase Gene and Escherichia coli Indicator Gene in a Microalgae-Based Wastewater Treatment System" Antibiotics 11, no. 11: 1531. https://doi.org/10.3390/antibiotics11111531