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Article

Antimicrobial Resistance Profiles and Genes of Staphylococci Isolated from Mastitic Cow’s Milk in Kenya

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Department of Veterinary Pathology, Microbiology and Parasitology, College of Agriculture and Veterinary Sciences, University of Nairobi, Nairobi P.O. Box 29053-00625, Kenya
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Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
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Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
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National Institute for Antimicrobial Resistance Research and Education, Ames, IA 50010, USA
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Centre for Biotechnology and Bioinformatics, University of Nairobi, Nairobi P.O. Box 30197-00100, Kenya
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Department of Clinical Studies, College of Agriculture and Veterinary Sciences, University of Nairobi, Nairobi P.O. Box 29053-00625, Kenya
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Department of Animal Production, College of Agriculture and Veterinary Sciences, University of Nairobi, Nairobi P.O. Box 29053-00625, Kenya
*
Author to whom correspondence should be addressed.
Academic Editor: Mark A. Holmes
Antibiotics 2021, 10(7), 772; https://doi.org/10.3390/antibiotics10070772
Received: 16 April 2021 / Revised: 7 June 2021 / Accepted: 15 June 2021 / Published: 24 June 2021
Increasing numbers of potentially zoonotic multidrug-resistant (MDR) staphylococci strains, associated with mastitis in dairy cows, are being reported globally and threaten disease management in both animal and human health. However, the prevalence and antimicrobial resistance profiles of these strains, including methicillin-resistant staphylococci (MRS), in Kenya is not well known. This study investigated the drug resistance profiles and genes carried by 183 staphylococci isolates from 142 dairy cows representing 93 farms recovered from mastitis milk of dairy cows in two selected counties in Kenya. Staphylococci isolates were characterized by phenotypic characteristics, polymerase chain reaction (PCR) amplification, partial sequencing and susceptibility testing for 10 antimicrobial drugs. Detection of seven resistance genes to the various antimicrobial drugs was conducted using PCR. Overall, phenotypic resistance among the staphylococci ranged between 66.1% for ampicillin and 3.5% for fluoroquinolones. Twenty-five percent (25%) of S. aureus and 10.8% of the coagulase-negative staphylococci (CoNS) isolates, were methicillin-resistant staphylococci phenotypically (defined as resistance to cefoxitin disk diffusion). The most common genes found in S. aureus and CoNS were blaZ and strB at 44.3% and 26%, and 78% and 50%, respectively. MDR was observed in 29.67% and 16.3% of S. aureus and CoNS, respectively. These findings pose a threat to bovine mastitis treatment and management as well as human health. View Full-Text
Keywords: methicillin-resistant staphylococci; bovine mastitis; antibiotic resistance genes; S. aureus coagulase-negative staphylococci methicillin-resistant staphylococci; bovine mastitis; antibiotic resistance genes; S. aureus coagulase-negative staphylococci
MDPI and ACS Style

Mbindyo, C.M.; Gitao, G.C.; Plummer, P.J.; Kulohoma, B.W.; Mulei, C.M.; Bett, R. Antimicrobial Resistance Profiles and Genes of Staphylococci Isolated from Mastitic Cow’s Milk in Kenya. Antibiotics 2021, 10, 772. https://doi.org/10.3390/antibiotics10070772

AMA Style

Mbindyo CM, Gitao GC, Plummer PJ, Kulohoma BW, Mulei CM, Bett R. Antimicrobial Resistance Profiles and Genes of Staphylococci Isolated from Mastitic Cow’s Milk in Kenya. Antibiotics. 2021; 10(7):772. https://doi.org/10.3390/antibiotics10070772

Chicago/Turabian Style

Mbindyo, Christine M., George C. Gitao, Paul Joseph Plummer, Benard W. Kulohoma, Charles M. Mulei, and Rawlynce Bett. 2021. "Antimicrobial Resistance Profiles and Genes of Staphylococci Isolated from Mastitic Cow’s Milk in Kenya" Antibiotics 10, no. 7: 772. https://doi.org/10.3390/antibiotics10070772

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