Transcription Factor EepR Is Required for Serratia marcescens Host Proinflammatory Response by Corneal Epithelial Cells
Abstract
:1. Introduction
2. Results
2.1. HCLE Cells Exposed to eepR Mutant S. marcescens Secretomes Have an Attenuated Inflammatory Response Compared with Wild-type Treated HCLE Cells
2.2. Bacterial Secretomes Influence Corneal Epithelial CellLipid Metabolism
3. Discussion
4. Materials and Methods
4.1. Bacterial Growth Conditions and Media
4.2. Microarray
4.3. Quantitative Reverse Transcriptase PCR (qPCR)
4.4. Metabolomics
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Mean of Normalized Expression, Duplicates | Expression Ratios | Scaled eepR | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Gene symbol | Entrez Gene number | LB control | WT Serratia | eepR mutant | WT/cont | eepR/cont | eepR/WT | WT/eepR | Biological Function | |
CXCL8 * | 3576 | 41 | 2346 | 145 | 56.6 | 3.5 | 0.1 | 16.2 | 0.04 | Inflammatory cytokine |
CXCL1 * | 2919 | 51 | 1452 | 566 | 28.4 | 11.1 | 0.4 | 2.6 | 0.37 | Inflammatory cytokine |
CCL20 * | 6364 | 297 | 8224 | 1757 | 27.7 | 5.9 | 0.2 | 4.7 | 0.18 | Inflammatory cytokine |
ITGB8 | 3696 | 4 | 91 | 38 | 25.7 | 10.8 | 0.4 | 2.4 | 0.40 | Integrin-mediated cell adhesion |
CXCL3 | 2921 | 35 | 830 | 108 | 23.7 | 3.1 | 0.1 | 7.7 | 0.09 | Chemotaxis |
GFPT2 | 9945 | 5 | 96 | 39 | 18.4 | 7.4 | 0.4 | 2.5 | 0.37 | Glutamine fructose-6-phosphate transaminase |
CSF2 * | 1437 | 74 | 1311 | 192 | 17.7 | 2.6 | 0.1 | 6.8 | 0.10 | granulocyte macrophage colony-stimulating factor receptor binding |
LIF | 3976 | 71 | 1164 | 93 | 16.4 | 1.3 | 0.1 | 12.5 | 0.02 | TGF Beta Signaling |
CSF3 | 1440 | 47 | 746 | 146 | 16.0 | 3.1 | 0.2 | 5.1 | 0.14 | granulocyte colony-stimulating factor receptor binding |
MMP1 * | 4312 | 104 | 1639 | 114 | 15.7 | 1.1 | 0.1 | 14.4 | 0.01 | Proteolysis |
CXCL2 | 2920 | 83 | 1163 | 428 | 14.1 | 5.2 | 0.4 | 2.7 | 0.32 | Chemokine |
MTSS1 | 9788 | 11 | 137 | 31 | 12.9 | 2.9 | 0.2 | 4.4 | 0.16 | Actin binding |
HCAR3 | 8843 | 306 | 3840 | 589 | 12.6 | 1.9 | 0.2 | 6.5 | 0.08 | G-protein coupled receptor signaling |
IL20 | 50604 | 31 | 379 | 26 | 12.3 | 0.8 | 0.1 | 14.6 | −0.01 | Receptor binding |
TNFAIP2 | 7127 | 20 | 236 | 198 | 11.7 | 9.9 | 0.8 | 1.2 | 0.83 | Angiogenesis |
ICAM1 * | 3383 | 40 | 445 | 217 | 11.3 | 5.5 | 0.5 | 2.1 | 0.44 | T cell antigen processing and presentation |
IL36G | 56300 | 92 | 1011 | 536 | 11.0 | 5.8 | 0.5 | 1.9 | 0.48 | Positive regulation of cytokine production |
SQSTM1 | 8878 | 8 | 81 | 28 | 10.7 | 3.7 | 0.3 | 2.9 | 0.28 | Positive regulation of protein phosphorylation |
MMP10 | 4319 | 100 | 1064 | 92 | 10.7 | 0.9 | 0.1 | 11.6 | −0.01 | Proteolysis |
PRDM1 | 639 | 139 | 1394 | 146 | 10.1 | 1.1 | 0.1 | 9.5 | 0.01 | Negative regulation of transcription from RNA polymerase II |
TRAF1 | 7185 | 16 | 160 | 54 | 10.0 | 3.4 | 0.3 | 3.0 | 0.27 | Apoptosis |
IL1R2 | 7850 | 66 | 640 | 412 | 9.7 | 6.2 | 0.6 | 1.6 | 0.60 | Immune response |
IL24 | 11009 | 474 | 4413 | 678 | 9.3 | 1.4 | 0.2 | 6.5 | 0.05 | Apoptosis |
MMP9 * | 4318 | 408 | 3792 | 2017 | 9.3 | 4.9 | 0.5 | 1.9 | 0.48 | Proteolysis |
IL6 * | 3569 | 60 | 545 | 152 | 9.1 | 2.5 | 0.3 | 3.6 | 0.19 | Inflammatory cytokine |
Mean of Normalized Expression, Duplicates | Expression Ratios | Scaled eepR | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | Entrez Gene number | LB control | WT Serratia | eepR mutant | WT/cont | eepR/cont | eepR/WT | WT/eepR | Biological Function | |
TXNIP | 10628 | 2659 | 64 | 320 | 0.02 | 0.1 | 5.0 | 0.2 | −0.90 | Negative regulation of transcription from RNA polymerase II |
CTGF | 1490 | 1245 | 55 | 35 | 0.04 | 0.0 | 0.6 | 1.6 | −1.02 | Cartilage condensation |
236865_at | --- | 117 | 7 | 24 | 0.06 | 0.2 | 3.5 | 0.3 | −0.84 | Unknown |
ARRDC4 | 91947 | 1338 | 96 | 285 | 0.07 | 0.2 | 3.0 | 0.3 | −0.85 | Positive regulation of ubiquitin-protein ligase activity |
LOC100287896 | 100287896 | 81 | 6 | 38 | 0.08 | 0.5 | 5.9 | 0.2 | −0.57 | Unknown |
NAP1L3 | 4675 | 35 | 4 | 31 | 0.10 | 0.9 | 8.6 | 0.1 | −0.14 | Nucleosome assembly |
RP4-813F11.4 | --- | 146 | 19 | 13 | 0.13 | 0.1 | 0.7 | 1.5 | −1.05 | Unknown |
HJURP | 55355 | 747 | 105 | 430 | 0.14 | 0.6 | 4.1 | 0.2 | −0.49 | Nucleosome assembly |
PIK3R3 | 8503 | 95 | 14 | 70 | 0.14 | 0.7 | 5.2 | 0.2 | −0.31 | Phospholipid metabolic process |
SLC26A7 | 115111 | 24 | 4 | 5 | 0.14 | 0.2 | 1.3 | 0.8 | −0.95 | Gastric acid secretion |
ARRDC3 | 57561 | 257 | 40 | 113 | 0.15 | 0.4 | 2.9 | 0.4 | −0.66 | Temperature homeostasis |
ZNF750 | 79755 | 148 | 24 | 62 | 0.16 | 0.4 | 2.6 | 0.4 | −0.69 | Transcription, DNA-dependent |
GPX8 | 493869 | 92 | 15 | 86 | 0.16 | 0.9 | 5.8 | 0.2 | −0.08 | Response to oxidative stress |
MECOM | 2122 | 154 | 25 | 65 | 0.16 | 0.4 | 2.6 | 0.4 | −0.69 | Neutrophil homeostasis |
ENC1 | 8507 | 379 | 64 | 169 | 0.17 | 0.4 | 2.6 | 0.4 | −0.67 | Multicellular organismal development |
THAP2 | 83591 | 88 | 15 | 11 | 0.17 | 0.1 | 0.7 | 1.4 | −1.05 | Nucleic acid binding |
1560973_a_at | --- | 34 | 6 | 16 | 0.18 | 0.5 | 2.7 | 0.4 | −0.63 | Unknown |
ZNF658 | 26149 | 56 | 10 | 56 | 0.19 | 1.0 | 5.4 | 0.2 | −0.00 | Transcription, DNA-dependent |
ST6GALNAC5 | 81849 | 76 | 14 | 44 | 0.19 | 0.6 | 3.1 | 0.3 | −0.51 | Protein glycosylation |
AOC3 | 8639 | 84 | 16 | 8 | 0.19 | 0.1 | 0.5 | 2.0 | −1.11 | Cell adhesion |
AKNAD1 | 254268 | 67 | 13 | 23 | 0.20 | 0.3 | 1.7 | 0.6 | −0.82 | Cytoplasm |
FAM83D | 81610 | 1588 | 313 | 1235 | 0.20 | 0.8 | 3.9 | 0.3 | −0.28 | Cell cycle |
242708_at | --- | 44 | 9 | 8 | 0.20 | 0.2 | 0.9 | 1.1 | −1.01 | Unknown |
ZC3H6 | 376940 | 99 | 20 | 32 | 0.21 | 0.3 | 1.6 | 0.6 | −0.85 | Nucleic acid binding |
* FAM72A | 554282 | 1976 | 413 | 1063 | 0.21 | 0.5 | 2.6 | 0.4 | −0.58 | Cytoplasm |
Mean of Normalized Expression, Duplicates | Expression Ratios | Scaled eepR | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | Entrez Gene number | LB control | WT Serratia | eepR mutant | WT/cont | eepR/cont | eepR/WT | WT/eepR | Biological Function | |
CXCL1 | 2919 | 51 | 1452 | 566 | 28.4 | 11.1 | 0.4 | 2.6 | 0.37 | Inflammatory cytokine |
ITGB8 | 3696 | 4 | 91 | 38 | 25.7 | 10.8 | 0.4 | 2.4 | 0.40 | Integrin-mediated cell adhesion |
TNFAIP2 | 7127 | 20 | 236 | 198 | 11.7 | 9.9 | 0.8 | 1.2 | 0.83 | Angiogenesis |
OLR1 | 4973 | 155 | 1302 | 1195 | 8.4 | 7.7 | 0.9 | 1.1 | 0.91 | Proteolysis |
IL1R2 | 7850 | 66 | 640 | 412 | 9.7 | 6.2 | 0.6 | 1.6 | 0.60 | Immune response |
CCL20 | 6364 | 297 | 8224 | 1757 | 27.7 | 5.9 | 0.2 | 4.7 | 0.18 | Inflammatory cytokine |
IL36G | 56300 | 92 | 1011 | 536 | 11.0 | 5.8 | 0.5 | 1.9 | 0.48 | Positive regulation of cytokine production |
SLC2A6 | 11182 | 34 | 97 | 189 | 2.9 | 5.6 | 1.9 | 0.5 | 2.45 | Transport |
ICAM1 | 3383 | 40 | 445 | 217 | 11.3 | 5.5 | 0.5 | 2.1 | 0.44 | T cell antigen processing and presentation |
CXCL2 | 2920 | 83 | 1163 | 428 | 14.1 | 5.2 | 0.4 | 2.7 | 0.32 | Chemokine |
MMP9 | 4318 | 408 | 3792 | 2017 | 9.3 | 4.9 | 0.5 | 1.9 | 0.48 | Proteolysis |
CXCL10 | 3627 | 115 | 211 | 533 | 1.8 | 4.6 | 2.5 | 0.4 | 4.36 | Positive regulation of leukocyte chemotaxis |
IL1R2 | 7850 | 53 | 435 | 241 | 8.3 | 4.6 | 0.6 | 1.8 | 0.49 | Immune response |
ICAM1 | 3383 | 47 | 367 | 213 | 7.9 | 4.6 | 0.6 | 1.7 | 0.52 | T cell antigen processing and presentation |
BIRC3 | 330 | 27 | 147 | 114 | 5.4 | 4.2 | 0.8 | 1.3 | 0.72 | Toll-like receptor signaling pathway |
SGPP2 | --- | 51 | 297 | 206 | 5.8 | 4.0 | 0.7 | 1.4 | 0.63 | Phospholipid metabolic process |
C15orf48 | 84419 | 26 | 92 | 99 | 3.5 | 3.8 | 1.1 | 0.9 | 1.11 | Mitochondrion |
JMJD4 | 65094 | 38 | 56 | 146 | 1.5 | 3.8 | 2.6 | 0.4 | 5.88 | Protein binding |
C6orf132 | 647024 | 42 | 140 | 159 | 3.3 | 3.8 | 1.1 | 0.9 | 1.19 | Unknown |
S100A7 | 6278 | 76 | 168 | 288 | 2.2 | 3.8 | 1.7 | 0.6 | 2.31 | Response to reactive oxygen species |
KMO | 8564 | 27 | 107 | 99 | 3.9 | 3.6 | 0.9 | 1.1 | 0.89 | Metabolic process |
EFNA1 | 1942 | 278 | 1973 | 985 | 7.1 | 3.5 | 0.5 | 2.0 | 0.42 | Negative regulation of transcription from RNA polymerase II promoter |
FAM20C | 56975 | 148 | 528 | 525 | 3.6 | 3.5 | 1.0 | 1.0 | 0.99 | Phosphorylation |
CXCL8 | 3576 | 41 | 2346 | 145 | 56.6 | 3.5 | 0.1 | 16.2 | 0.04 | Inflammatory cytokine |
KMO | 8564 | 28 | 121 | 96 | 4.4 | 3.5 | 0.8 | 1.3 | 0.74 | Metabolic process |
Mean of Normalized Expression, Duplicates | Expression Ratios | Scaled eepR | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | Entrez Gene number | LB control | WT Serratia | eepR mutant | WT/cont | eepR/cont | eepR/WT | WT/eepR | Biological Function | |
CTGF | 1490 | 1245 | 55 | 35 | 0.04 | 0.03 | 0.64 | 1.6 | −1.02 | Cartilage condensation |
RP4-813F11.4 | --- | 146 | 19 | 13 | 0.13 | 0.09 | 0.69 | 1.5 | −1.05 | Unknown |
AOC3 | 8639 | 84 | 16 | 8 | 0.19 | 0.10 | 0.51 | 2.0 | −1.11 | Cell adhesion |
SERPINE1 | 5054 | 81 | 42 | 9 | 0.52 | 0.11 | 0.22 | 4.7 | −1.86 | Regulation of mRNA stability |
TXNIP | 10628 | 2659 | 64 | 320 | 0.02 | 0.12 | 5.01 | 0.2 | −0.90 | Negative regulation of transcription from RNA polymerase II |
THAP2 | 83591 | 88 | 15 | 11 | 0.17 | 0.13 | 0.74 | 1.4 | −1.05 | Nucleic acid binding |
SLC6A13 | 6540 | 122 | 46 | 18 | 0.38 | 0.15 | 0.39 | 2.6 | −1.37 | Transport |
RFPL3S | 10737 | 31 | 11 | 6 | 0.35 | 0.18 | 0.53 | 1.8 | −1.25 | Unknown |
242708_at | --- | 44 | 9 | 8 | 0.20 | 0.19 | 0.95 | 1.1 | −1.01 | Unknown |
SLC26A7 | 115111 | 24 | 4 | 5 | 0.14 | 0.19 | 1.31 | 0.8 | −0.95 | Gastric acid secretion |
EGR3 | 1960 | 567 | 602 | 108 | 1.06 | 0.19 | 0.18 | 5.6 | −12.90 | Positive regulation of endothelial cell proliferation |
SERTAD4 | 56256 | 40 | 14 | 8 | 0.35 | 0.20 | 0.58 | 1.8 | −1.23 | Unknown |
236865_at | --- | 117 | 7 | 24 | 0.06 | 0.21 | 3.55 | 0.3 | −0.84 | Unknown |
ARRDC4 | 91947 | 1338 | 96 | 285 | 0.07 | 0.21 | 2.97 | 0.3 | −0.85 | Temperature homeostasis |
MYEF2 | 50804 | 37 | 5 | 8 | 0.14 | 0.23 | 1.65 | 0.6 | −0.90 | Transcription, DNA-dependent |
RYBP | 23429 | 63 | 31 | 15 | 0.49 | 0.24 | 0.49 | 2.1 | −1.49 | Negative regulation of transcription from RNA polymerase II promoter |
238548_at | 238548_at | 44 | 19 | 11 | 0.43 | 0.25 | 0.59 | 1.7 | −1.31 | Unknown |
LOC100130705 | 100130705 | 67 | 29 | 17 | 0.43 | 0.26 | 0.60 | 1.7 | −1.30 | Unknown |
CYR61 | 3491 | 5396 | 1506 | 1419 | 0.28 | 0.26 | 0.94 | 1.1 | −1.02 | Regulation of cell growth |
ZBTB1 | 22890 | 396 | 217 | 108 | 0.55 | 0.27 | 0.50 | 2.0 | −1.61 | Transcription, DNA-dependent |
FOS | 2353 | 425 | 496 | 117 | 1.17 | 0.28 | 0.24 | 4.2 | −4.34 | Toll-like receptor signaling pathway |
BC034636 /// CTB-113P19.4 | --- | 53 | 18 | 15 | 0.34 | 0.28 | 0.81 | 1.2 | −1.10 | Unknown |
ANGPTL4 | 51129 | 393 | 89 | 111 | 0.23 | 0.28 | 1.25 | 0.8 | −0.93 | Angiogenesis |
UQCRB | 7381 | 47 | 14 | 14 | 0.30 | 0.30 | 1.02 | 1.0 | −0.99 | Oxidative phosphorylation |
C1orf52 | 148423 | 171 | 56 | 52 | 0.33 | 0.30 | 0.93 | 1.1 | −1.04 | Unknown |
Mean of Normalized Expression, Duplicates | Expression Ratios | Scaled eepR | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | Entrez Gene number | LB control | WT Serratia | eepR mutant | WT/cont | eepR/cont | eepR/WT | WT/eepR | Biological Function | |
TOMM40L | 84134 | 72 | 71 | 237 | 0.99 | 3.29 | 3.33 | 0.3 | 235.4 | Transport |
ARL11 | 115761 | 18 | 19 | 51 | 1.01 | 2.75 | 2.72 | 0.4 | 161.0 | Intracellular protein transport |
IGFL1 | 374918 | 170 | 173 | 420 | 1.02 | 2.47 | 2.43 | 0.4 | 83.3 | Protein binding |
227356_at | --- | 109 | 112 | 182 | 1.02 | 1.66 | 1.63 | 0.6 | 30.8 | Unknown |
TMEM177 | 80775 | 125 | 120 | 221 | 0.96 | 1.76 | 1.84 | 0.5 | 18.5 | Membrane |
TRIM14 | 9830 | 221 | 212 | 376 | 0.96 | 1.70 | 1.77 | 0.6 | 17.8 | Protein binding |
ZSCAN16 | 80345 | 54 | 52 | 97 | 0.95 | 1.78 | 1.86 | 0.5 | 17.2 | Transcription, DNA-dependent |
RITA1 | 84934 | 80 | 75 | 157 | 0.94 | 1.97 | 2.10 | 0.5 | 15.1 | Intracellular protein transport |
KRT34 /// LOC100653049 | 3885 /// 100653049 | 202 | 220 | 463 | 1.09 | 2.29 | 2.11 | 0.5 | 14.8 | Epidermis development |
CTSC | 1075 | 69 | 64 | 135 | 0.93 | 1.97 | 2.11 | 0.5 | 13.7 | T cell mediated cytotoxicity |
FAM13B | 51306 | 128 | 121 | 218 | 0.95 | 1.70 | 1.80 | 0.6 | 13.1 | Signal transduction |
CCDC8 | 83987 | 70 | 58 | 215 | 0.83 | 3.08 | 3.73 | 0.3 | 12.1 | Negative regulation of phosphatase activity |
KIAA1586 | 57691 | 34 | 37 | 65 | 1.08 | 1.91 | 1.77 | 0.6 | 11.9 | Nucleic acid binding |
COG8 /// PDF | 64146 /// 84342 | 199 | 217 | 384 | 1.09 | 1.93 | 1.77 | 0.6 | 10.7 | Translation |
MTRR | 4552 | 418 | 449 | 708 | 1.07 | 1.69 | 1.58 | 0.6 | 9.4 | Sulfur amino acid metabolic process |
SLC35F6 | 54978 | 125 | 141 | 269 | 1.13 | 2.15 | 1.91 | 0.5 | 9.1 | Establishment of mitotic spindle orientation |
CXCL11 | 6373 | 121 | 100 | 287 | 0.83 | 2.38 | 2.87 | 0.3 | 8.1 | Positive regulation of leukocyte chemotaxis |
HSD17B1 | 3292 | 83 | 103 | 234 | 1.24 | 2.82 | 2.28 | 0.4 | 7.6 | Lipid metabolic process |
LOC284926 | 284926 | 8 | 13 | 44 | 1.63 | 5.55 | 3.41 | 0.3 | 7.2 | Unknown |
NOP56 | 10528 | 206 | 233 | 384 | 1.13 | 1.87 | 1.65 | 0.6 | 6.5 | rRNA processing |
* FAM86B1 | *55199 | 32 | 26 | 65 | 0.82 | 2.07 | 2.51 | 0.4 | 6.1 | Unknown |
JMJD4 | 65094 | 38 | 56 | 146 | 1.48 | 3.82 | 2.58 | 0.4 | 5.9 | Protein binding |
PPAPDC2 | 403313 | 67 | 85 | 156 | 1.26 | 2.33 | 1.84 | 0.5 | 5.0 | Metabolic process |
AIMP2 | 7965 | 1059 | 966 | 1505 | 0.91 | 1.42 | 1.56 | 0.6 | 4.8 | Translation |
ZNF165 | 7718 | 184 | 220 | 358 | 1.20 | 1.95 | 1.63 | 0.6 | 4.8 | Transcription, DNA-dependent |
Mean of Normalized Expression, Duplicates | Expression Ratios | Scaled eepR | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | Entrez Gene number | LB control | WT Serratia | eepR mutant | WT/cont | eepR/cont | eepR/WT | WT/eepR | (eepR—cont) |(WT—cont)| | Biological Function |
NUFIP2 | 57532 | 1790 | 1786 | 1204 | 1.00 | 0.67 | 0.67 | 1.5 | −144.7 | Protein binding |
ZFP36L2 | 678 | 3551 | 3536 | 2305 | 1.00 | 0.65 | 0.65 | 1.5 | −83.9 | Regulation of transcription, DNA dependent |
TUFT1 | 7286 | 1322 | 1329 | 818 | 1.01 | 0.62 | 0.62 | 1.6 | −74.7 | Protein binding |
PARD6B | 84612 | 495 | 491 | 230 | 0.99 | 0.46 | 0.47 | 2.1 | −59.7 | Protein complex assembly |
GPR157 | 80045 | 364 | 359 | 208 | 0.99 | 0.57 | 0.58 | 1.7 | −33.0 | Signal transduction |
ARPC5L | 81873 | 1049 | 1062 | 602 | 1.01 | 0.57 | 0.57 | 1.8 | −32.4 | Regulation of actin filament polymerization |
JUN | 3725 | 1312 | 1338 | 621 | 1.02 | 0.47 | 0.46 | 2.2 | −27.1 | Angiogenesis |
DUSP6 | 1848 | 3965 | 3858 | 1638 | 0.97 | 0.41 | 0.42 | 2.4 | −21.6 | Inactivation of MAPK activity |
CD274 | 29126 | 373 | 386 | 184 | 1.04 | 0.49 | 0.48 | 2.1 | −14.4 | Immune response |
EGR3 | 1960 | 567 | 602 | 108 | 1.06 | 0.19 | 0.18 | 5.6 | −12.9 | Positive regulation of endothelial cell proliferation |
1555897_at | --- | 89 | 85 | 47 | 0.96 | 0.53 | 0.55 | 1.8 | −11.9 | Unknown |
CHMP1B | 57132 | 220 | 227 | 145 | 1.03 | 0.66 | 0.64 | 1.6 | −11.4 | Cytokinesis |
FHL2 | 2274 | 2117 | 2058 | 1460 | 0.97 | 0.69 | 0.71 | 1.4 | −11.3 | Negative regulation of transcription from RNA polymerase II promoter |
E2F7 | 144455 | 1501 | 1411 | 612 | 0.94 | 0.41 | 0.43 | 2.3 | −10.0 | Negative regulation of transcription from RNA polymerase II promoter |
SLC2A14 /// SLC2A3 | 6515 /// 144195 | 358 | 342 | 203 | 0.96 | 0.57 | 0.59 | 1.7 | −9.8 | Carbohydrate metabolic process |
PHF13 | 148479 | 631 | 661 | 347 | 1.05 | 0.55 | 0.52 | 1.9 | −9.5 | Mitotic cell cycle |
JAG1 | 182 | 3839 | 3973 | 2726 | 1.03 | 0.71 | 0.69 | 1.5 | −8.3 | Angiogenesis |
SERTAD1 | 29950 | 1254 | 1334 | 653 | 1.06 | 0.52 | 0.49 | 2.0 | −7.5 | Regulation of cyclin-dependent protein serine/threonine kinase activity |
KIAA0907 | 22889 | 1868 | 1755 | 1034 | 0.94 | 0.55 | 0.59 | 1.7 | −7.4 | Unknown |
SOS1 | 6654 | 442 | 467 | 268 | 1.06 | 0.61 | 0.58 | 1.7 | −7.1 | Apoptotic process |
C16orf72 | 29035 | 2072 | 2169 | 1411 | 1.05 | 0.68 | 0.65 | 1.5 | −6.8 | Unknown |
RND3 | 390 | 1928 | 1794 | 1126 | 0.93 | 0.58 | 0.63 | 1.6 | −6.0 | GTP catabolic process |
SMAD7 | 4092 | 271 | 290 | 158 | 1.07 | 0.58 | 0.54 | 1.8 | −5.7 | Negative regulation of transcription from RNA polymerase II promoter |
ADAMTS6 | 11174 | 171 | 187 | 86 | 1.10 | 0.50 | 0.46 | 2.2 | −5.1 | Proteolysis |
FZD7 | 8324 | 63 | 60 | 31 | 0.89 | 0.45 | 0.50 | 2.0 | −5.0 | Wnt signaling |
Canonical Pathway. | −log(p-Value) | Number Genes up-Regulated | Number Genes down-Regulated | Total Genes in Pathway |
---|---|---|---|---|
IL-6 Signaling | 8.4 | 20 | 1 | 116 |
Toll-like Receptor Signaling | 7.9 | 16 | 1 | 72 |
NF-kB Signaling | 7.0 | 22 | 1 | 164 |
Colorectal Cancer Metastasis Signaling | 5.4 | 20 | 1 | 230 |
PPAR Signaling | 5.0 | 14 | 1 | 90 |
TREM1 Signaling | 4.9 | 12 | 1 | 69 |
HMGB1 Signaling | 4.8 | 15 | 1 | 118 |
Acute Phase Response Signaling | 4.6 | 18 | 1 | 166 |
Role of Pattern Recognition Receptors in Recognition of Bacteria and Viruses | 4.2 | 13 | 1 | 118 |
Cholecystokinin/Gastrin-mediated Signaling | 3.3 | 11 | 1 | 99 |
B Cell Activating Factor Signaling | 3.1 | 7 | 1 | 40 |
LXR/RXR Activation | 3.1 | 12 | 1 | 120 |
Pancreatic Adenocarcinoma Signaling | 3.0 | 10 | 1 | 106 |
Glioma Invasiveness Signaling | 2.8 | 7 | 1 | 57 |
Cell Cycle: G2/M DNA Damage Checkpoint Regulation | 2.6 | 1 | 1 | 49 |
NF-kB Activation by Viruses | 2.1 | 7 | 1 | 73 |
NRF2-mediated Oxidative Stress Response | 2.0 | 12 | 1 | 175 |
CXCL8 Signaling | 1.9 | 13 | 1 | |
Tec Kinase Signaling | 1.8 | 10 | 2 | 183 |
MIF Regulation of Innate Immunity | 1.8 | 5 | 0 | 150 |
iNOS Signaling | 1.6 | 5 | 0 | 39 |
Antioxidant Action of Vitamin C | 1.6 | 8 | 0 | 43 |
PPARα/RXRα Activation | 1.5 | 12 | 0 | 91 |
Phospholipase C Signaling | 1.3 | 13 | 1 | 165 |
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Brothers, K.M.; Harvey, S.A.K.; Shanks, R.M.Q. Transcription Factor EepR Is Required for Serratia marcescens Host Proinflammatory Response by Corneal Epithelial Cells. Antibiotics 2021, 10, 770. https://doi.org/10.3390/antibiotics10070770
Brothers KM, Harvey SAK, Shanks RMQ. Transcription Factor EepR Is Required for Serratia marcescens Host Proinflammatory Response by Corneal Epithelial Cells. Antibiotics. 2021; 10(7):770. https://doi.org/10.3390/antibiotics10070770
Chicago/Turabian StyleBrothers, Kimberly M., Stephen A. K. Harvey, and Robert M. Q. Shanks. 2021. "Transcription Factor EepR Is Required for Serratia marcescens Host Proinflammatory Response by Corneal Epithelial Cells" Antibiotics 10, no. 7: 770. https://doi.org/10.3390/antibiotics10070770