Speciation and Antibiotic Susceptibilities of Coagulase Negative Staphylococci Isolated from Ocular Infections
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Coagulase-Negative Staphylococci
4.2. Antibiotic Susceptibility Testing of CoNS
4.3. API Staph
4.4. Biolog
4.5. DNA Sequencing
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Isolated from Endophthalmitis | API Staph | Biolog | Sequencing | Correlation of ID Tests | |
---|---|---|---|---|---|
n (%) | n (%) | n (%) | 3 of 3 | 2 of 3 | |
S. epidermidis | 42 (84) | 44 (88) | 45 (90) | 41 | 3 |
S. hominis | 3 (6) | 1 (2) | 1 (2) | 0 | 0 |
S. lugdunensis | 2 (4) | 4 (8) | 3 (6) | 0 | 3 |
S. haemolyticus | 1 (2) | 1 (2) | 1 (2) | 1 | 0 |
S. capitis | 1 (2) | 0 (0) | 0 (0) | ||
S. aureus | 1 (2) | 0 (0) | 0 (0) | ||
Isolated from Keratitis | |||||
S. epidermidis | 40 (80) | 40 (80) | 43 (86) | 37 | 4 |
S. caprae | 3 (6) | 0 (0) | 1 (2) | 0 | 1 |
S. hominis | 2 (4) | 3 (6) | 0 (0) | 0 | 1 |
S. warneri | 2 (4) | 0 (0) | 1 (2) | 0 | 1 |
S. lugdunensis | 1 (2) | 1 (2) | 1 (2) | 0 | 2 |
S. aureus | 1 (2) | 0 (0) | 1 (2) | 0 | 2 |
S. capitis | 0 (0) | 4 (8) | 2 (4) | ||
S. pasteuri | 0 (0) | 2 (4) | 0 (0) | ||
S. pettenkoferi | 0 (0) | 0 (0) | 1 (2) | ||
Micrococcus species | 1 (2) | 0 (0) | 0 (0) | ||
Isolated from Conjunctivitis/Blepharitis | |||||
S. epidermidis | 31 (62) | 33 (66) | 34 (68) | 28 | 6 |
S. aureus | 6 (12) | 0 (0) | 0 (0) | ||
S. haemolyticus | 2 (4) | 3 (6) | 5 (10) | 2 | 0 |
S. hominis | 2 (4) | 2 (4) | 2 (4) | 0 | 2 |
S. lugdunensis | 2(4) | 4 (8) | 2 (4) | 1 | 2 |
S. warneri | 2 (4) | 1 (2) | 2 (4) | 0 | 1 |
S. capitis | 1 (2) | 3 (6) | 1 (2) | 1 | 0 |
S. caprae | 1 (2) | 1 (2) | 2 (4) | 0 | 2 |
S. chromogenes | 1 (2) | 0 (0) | 0 (0) | ||
S. cohnii | 1 (2) | 1 (2) | 1 (2) | 1 | 0 |
S. sciuri | 1 (2) | 0 (0) | 0 (0) | ||
S. pasteuri | 0 (0) | 1 (2) | 1 (2) | ||
S. saprophyticus | 0 (0) | 1 (2) | 0 (0) |
CoNS (Number Identified) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Endophthalmitis | VA | GM | CIP | OFX | CZ | AMK | CAZ | CC | MXF | FOX | |
S. epidermidis (44) | 100 | 93.2 | 47.7 | 45.5 | 100 | 97.7 | 81.8 | 84.1 | 65.9 | 68.2 | |
S. lugdunensis (4) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | |
S. hominis (1) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | |
S. haemolyticus (1) | 100 | 100 | 0 | 0 | 100 | 100 | 0 | 0 | 0 | 0 | |
Total (50) | 100 | 94 | 52 | 50 | 100 | 98 | 82 | 84 | 68 | 70 | |
Keratitis | BAC | VA | GM | CIP | OFX | PB | CZ | TOB | Sulfa | MXF | FOX |
S. epidermidis (40) | 75 | 100 | 87.5 | 50 | 50 | 82.5 | 97.5 | 85 | 82.5 | 67.5 | 57.5 |
S. capitis (4) | 100 | 100 | 75 | 75 | 75 | 100 | 100 | 100 | 100 | 100 | 100 |
S. hominis (3) | 66.7 | 100 | 66.7 | 33.3 | 33.3 | 100 | 100 | 66.7 | 66.7 | 66.7 | 66.7 |
S. pasteuri (2) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
S. lugdunensis (1) | 0 | 100 | 100 | 0 | 0 | 100 | 100 | 100 | 100 | 0 | 0 |
Total (50) | 76 | 100 | 86 | 52 | 52 | 86 | 98 | 86 | 86 | 70 | 64 |
Conjunctivitis/ Blepharitis | BAC | ERYT | GM | CIP | OFX | TMP | PB | TOB | Sulfa | MXF | |
S. epidermidis (34) | 79.4 | 26.5 | 70.6 | 47.1 | 47.1 | 50 | 85.3 | 70.6 | 79.4 | 29.4 | |
S. lugdunensis (3) | 66.7 | 0 | 100 | 66.7 | 66.7 | 33.3 | 100 | 100 | 66.7 | 66.7 | |
S. hominis (2) | 100 | 0 | 100 | 50 | 50 | 0 | 50 | 100 | 100 | 50 | |
S. haemolyticus (3) | 66.7 | 0 | 33.3 | 0 | 0 | 0 | 100 | 0 | 33.3 | 0 | |
S. cohnii (1) | 0 | 0 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | |
S. saprophyticus (1) | 0 | 0 | 100 | 100 | 100 | 100 | 0 | 100 | 100 | 100 | |
S. capitis (3) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 66.7 | |
S. pasteuri (1) | 100 | 100 | 0 | 100 | 100 | 0 | 0 | 0 | 100 | 0 | |
S. warneri (1) | 100 | 100 | 100 | 0 | 0 | 100 | 100 | 100 | 100 | 0 | |
S. caprae (1) | 100 | 0 | 100 | 0 | 0 | 0 | 0 | 100 | 100 | 100 | |
Total (50) | 78 | 30 | 74 | 50 | 50 | 48 | 82 | 72 | 80 | 36 |
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Romanowski, J.E.; Nayyar, S.V.; Romanowski, E.G.; Jhanji, V.; Shanks, R.M.Q.; Kowalski, R.P. Speciation and Antibiotic Susceptibilities of Coagulase Negative Staphylococci Isolated from Ocular Infections. Antibiotics 2021, 10, 721. https://doi.org/10.3390/antibiotics10060721
Romanowski JE, Nayyar SV, Romanowski EG, Jhanji V, Shanks RMQ, Kowalski RP. Speciation and Antibiotic Susceptibilities of Coagulase Negative Staphylococci Isolated from Ocular Infections. Antibiotics. 2021; 10(6):721. https://doi.org/10.3390/antibiotics10060721
Chicago/Turabian StyleRomanowski, John E., Shannon V. Nayyar, Eric G. Romanowski, Vishal Jhanji, Robert M. Q. Shanks, and Regis P. Kowalski. 2021. "Speciation and Antibiotic Susceptibilities of Coagulase Negative Staphylococci Isolated from Ocular Infections" Antibiotics 10, no. 6: 721. https://doi.org/10.3390/antibiotics10060721