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Volume 10
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Article

Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota

by
Pedro Miguela-Villoldo
1,2,
Miguel A. Moreno
1,2,
Agustín Rebollada-Merino
1,3,
Antonio Rodríguez-Bertos
1,3,
Marta Hernández
4,
David Rodríguez-Lázaro
5,
Alejandro Gallardo
6,
Alberto Quesada
6,7,
Joaquín Goyache
1,2,
Lucas Domínguez
1,2 and
María Ugarte-Ruiz
1,*
1
VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, Spain
2
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, Spain
3
Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, Spain
4
Laboratorio de Biología Molecular y Microbiología, Instituto Tecnológico Agrario de Castilla y León, Ctra. Burgos Km. 119, 47071 Valladolid, Spain
5
Área de Microbiología, Departamento de Biotecnología y Ciencia de los Alimentos, Facultad de Ciencias, Universidad de Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain
6
Departamento de Bioquímica, Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad s/n, 10003 Caceres, Spain
7
INBIO G+C, Universidad de Extremadura, 0004 Caceres, Spain
*
Author to whom correspondence should be addressed.
Antibiotics 2021, 10(6), 677; https://doi.org/10.3390/antibiotics10060677
Submission received: 7 May 2021 / Revised: 24 May 2021 / Accepted: 3 June 2021 / Published: 5 June 2021
(This article belongs to the Special Issue Antibiotic Resistance and Infectious Diseases: A One Health Perspective)
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Abstract

Colistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the mcr-1 gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed to assess the effect of colistin administration on the dispersion of resistance in the microbiota of day-old broiler chicks and how the presence of mcr-1 genes influences the spread of colistin resistance determinants. In this study, 100 one-day-old chicks were divided into four groups of 25 animals (G1, G2, G3, and G4). Animals from G3/G4 were challenged with mcr-1-carrying Salmonella (day 7), while colistin (600 mg/L) was administered daily to G2/G4 animals through drinking water (from day 8 to day 15). Two quantitative PCR assays were performed to compare the amount of Salmonella and mcr-1 that were present in the caecal samples. We observed that levels of mcr-1 were higher in G3/G4 animals, especially G4, due to the spread of mcr-1-carrying Salmonella. On day 21, Salmonella levels decreased in G4, reaching similar values as those for G3, but mcr-1 levels remained significantly higher, suggesting that colistin may accelerate the spreading process when mcr-1-carrying bacteria reach the gut.
Keywords: colistin; mcr-1; Salmonella; in vivo assay; real-time PCR quantification; microbiota; chicks colistin; mcr-1; Salmonella; in vivo assay; real-time PCR quantification; microbiota; chicks

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MDPI and ACS Style

Miguela-Villoldo, P.; Moreno, M.A.; Rebollada-Merino, A.; Rodríguez-Bertos, A.; Hernández, M.; Rodríguez-Lázaro, D.; Gallardo, A.; Quesada, A.; Goyache, J.; Domínguez, L.; et al. Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota. Antibiotics 2021, 10, 677. https://doi.org/10.3390/antibiotics10060677

AMA Style

Miguela-Villoldo P, Moreno MA, Rebollada-Merino A, Rodríguez-Bertos A, Hernández M, Rodríguez-Lázaro D, Gallardo A, Quesada A, Goyache J, Domínguez L, et al. Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota. Antibiotics. 2021; 10(6):677. https://doi.org/10.3390/antibiotics10060677

Chicago/Turabian Style

Miguela-Villoldo, Pedro, Miguel A. Moreno, Agustín Rebollada-Merino, Antonio Rodríguez-Bertos, Marta Hernández, David Rodríguez-Lázaro, Alejandro Gallardo, Alberto Quesada, Joaquín Goyache, Lucas Domínguez, and et al. 2021. "Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota" Antibiotics 10, no. 6: 677. https://doi.org/10.3390/antibiotics10060677

APA Style

Miguela-Villoldo, P., Moreno, M. A., Rebollada-Merino, A., Rodríguez-Bertos, A., Hernández, M., Rodríguez-Lázaro, D., Gallardo, A., Quesada, A., Goyache, J., Domínguez, L., & Ugarte-Ruiz, M. (2021). Colistin Selection of the Mcr-1 Gene in Broiler Chicken Intestinal Microbiota. Antibiotics, 10(6), 677. https://doi.org/10.3390/antibiotics10060677

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