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Antibiotics
Volume 10
Issue 4
10.3390/antibiotics10040375
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Article

Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model

by
Chani Rakov
1,
Shira Ben Porat
1,
Sivan Alkalay-Oren
1,
Ortal Yerushalmy
1,
Mohanad Abdalrhman
2,
Niv Gronovich
1,
Lina Huang
3,
David Pride
3,
Shunit Coppenhagen-Glazer
1,
Ran Nir-Paz
2 and
Ronen Hazan
1,*
1
Institute of Dental Sciences, School of Dentistry, Hebrew University of Jerusalem, Jerusalem 91120, Israel
2
Hadassah-Hebrew University Medical Center, Department of Clinical Microbiology and Infectious Diseases, The Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 91120, Israel
3
Department of Pathology, University of California, San Diego, CA 92093, USA
*
Author to whom correspondence should be addressed.
Antibiotics 2021, 10(4), 375; https://doi.org/10.3390/antibiotics10040375
Submission received: 8 March 2021 / Revised: 21 March 2021 / Accepted: 24 March 2021 / Published: 2 April 2021
(This article belongs to the Special Issue Benefits of Bacteriophages to Combat Antibiotic-Resistant Bacteria)
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Abstract

Providencia spp. are emerging pathogens mainly in nosocomial infections. Providencia stuartii in particular is involved in urinary tract infections and contributes significantly to the high incidence of biofilm-formation in catheterized patients. Furthermore, recent reports suggested a role for multiple drug resistant (MDR) P. stuartii in hospital-associated outbreaks which leads to excessive complications resulting in challenging treatments. Phage therapy is currently one of the most promising solutions to combat antibiotic-resistant infections. However, the number of available phages targeting Providencia spp. is extremely limited, restricting the use of phage therapy in such cases. In the present study, we describe the isolation and characterization of 17 lytic and temperate bacteriophages targeting clinical isolates of Providencia spp. as part of the Israeli Phage Bank (IPB). These phages, isolated from sewage samples, were evaluated for host range activity and effectively eradicated 95% of the tested bacterial strains isolated from different geographic locations and displaying a wide range of antibiotic resistance. Their lytic activity is demonstrated on agar plates, planktonic cultures, and biofilm formed in a catheter model. The results suggest that these bacteriophages can potentially be used for treatment of antibiotic-resistant Providencia spp. infections in general and of urinary tract infections in particular.
Keywords: phage therapy; antibiotic resistance; Providencia stuartii; Providencia rettgeri; catheter infections phage therapy; antibiotic resistance; Providencia stuartii; Providencia rettgeri; catheter infections

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MDPI and ACS Style

Rakov, C.; Ben Porat, S.; Alkalay-Oren, S.; Yerushalmy, O.; Abdalrhman, M.; Gronovich, N.; Huang, L.; Pride, D.; Coppenhagen-Glazer, S.; Nir-Paz, R.; et al. Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model. Antibiotics 2021, 10, 375. https://doi.org/10.3390/antibiotics10040375

AMA Style

Rakov C, Ben Porat S, Alkalay-Oren S, Yerushalmy O, Abdalrhman M, Gronovich N, Huang L, Pride D, Coppenhagen-Glazer S, Nir-Paz R, et al. Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model. Antibiotics. 2021; 10(4):375. https://doi.org/10.3390/antibiotics10040375

Chicago/Turabian Style

Rakov, Chani, Shira Ben Porat, Sivan Alkalay-Oren, Ortal Yerushalmy, Mohanad Abdalrhman, Niv Gronovich, Lina Huang, David Pride, Shunit Coppenhagen-Glazer, Ran Nir-Paz, and et al. 2021. "Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model" Antibiotics 10, no. 4: 375. https://doi.org/10.3390/antibiotics10040375

APA Style

Rakov, C., Ben Porat, S., Alkalay-Oren, S., Yerushalmy, O., Abdalrhman, M., Gronovich, N., Huang, L., Pride, D., Coppenhagen-Glazer, S., Nir-Paz, R., & Hazan, R. (2021). Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model. Antibiotics, 10(4), 375. https://doi.org/10.3390/antibiotics10040375

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