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Article

In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov.

1
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba 292-0818, Japan
2
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Tokyo 151-0066, Japan
3
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama 939-0398, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Nicholas Dixon
Antibiotics 2021, 10(12), 1447; https://doi.org/10.3390/antibiotics10121447
Received: 31 October 2021 / Revised: 19 November 2021 / Accepted: 22 November 2021 / Published: 25 November 2021
(This article belongs to the Special Issue 10th Anniversary of Antibiotics—Feature Papers)
Micromonospora sp. TP-A0316 and Micromonospora sp. TP-A0468 are producers of arisostatin and kosinostatin, respectively. Micromonospora sp. TP-A0316 showed a 16S rRNA gene sequence similarity of 100% to Micromonosporaoryzae CP2R9-1T whereas Micromonospora sp. TP-A0468 showed a 99.3% similarity to Micromonospora haikouensis 232617T. A phylogenetic analysis based on gyrB sequences suggested that Micromonospora sp. TP-A0316 is closely related to Micromonospora oryzae whereas Micromonospora TP-A0468 is an independent genomospecies. As Micromonospora sp. TP-A0468 showed some phenotypic differences to its closely related species, it was classified as a novel species, for which the name Micromonospora okii sp. nov. is proposed. The type strain is TP-A0468T (= NBRC 110461T). Micromonospora sp. TP-A0316 and M. okii TP-A0468T were both found to harbor 15 gene clusters for secondary metabolites such as polyketides and nonribosomal peptides in their genomes. Arisostatin-biosynthetic gene cluster (BGC) of Micromonospora sp. TP-A0316 closely resembled tetrocarcin A-BGC of Micromonospora chalcea NRRL 11289. A large type-I polyketide synthase gene cluster was present in each genome of Micromonospora sp. TP-A0316 and M. okii TP-A0468T. It was an ortholog of quinolidomicin-BGC of M. chalcea AK-AN57 and widely distributed in the genus Micromonospora. View Full-Text
Keywords: arisostatin; classification; kosinostatin; Micromonospora; polyketide; quinolidomicin; secondary metabolite arisostatin; classification; kosinostatin; Micromonospora; polyketide; quinolidomicin; secondary metabolite
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MDPI and ACS Style

Komaki, H.; Ichikawa, N.; Hosoyama, A.; Hamada, M.; Igarashi, Y. In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov. Antibiotics 2021, 10, 1447. https://doi.org/10.3390/antibiotics10121447

AMA Style

Komaki H, Ichikawa N, Hosoyama A, Hamada M, Igarashi Y. In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov. Antibiotics. 2021; 10(12):1447. https://doi.org/10.3390/antibiotics10121447

Chicago/Turabian Style

Komaki, Hisayuki, Natsuko Ichikawa, Akira Hosoyama, Moriyuki Hamada, and Yasuhiro Igarashi. 2021. "In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov." Antibiotics 10, no. 12: 1447. https://doi.org/10.3390/antibiotics10121447

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