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Open AccessArticle

Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)—An Electrochemical Optimisation Approach

Department of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UK
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Biosensors 2019, 9(4), 141; https://doi.org/10.3390/bios9040141
Received: 1 November 2019 / Revised: 28 November 2019 / Accepted: 4 December 2019 / Published: 6 December 2019
We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open-circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and noncomplementary DNA target sequences. View Full-Text
Keywords: field-effect transistor; biosensor; TP53; electrochemistry; open-circuit potential field-effect transistor; biosensor; TP53; electrochemistry; open-circuit potential
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Crossley, L.; Attoye, B.; Vezza, V.; Blair, E.; Corrigan, D.K.; Hannah, S. Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)—An Electrochemical Optimisation Approach. Biosensors 2019, 9, 141.

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