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Open AccessArticle

Development of Microalgae Biosensor Chip by Incorporating Microarray Oxygen Sensor for Pesticides Sensing

Department of Electric and Electronic Engineering, Faculty of Engineering, University of Toyama, Toyama 930-8555, Japan
Author to whom correspondence should be addressed.
Biosensors 2019, 9(4), 133;
Received: 12 September 2019 / Revised: 8 November 2019 / Accepted: 8 November 2019 / Published: 12 November 2019
(This article belongs to the Special Issue Dedication to Professor Isao Karube: Microbial Biosensors)
A microalgae (Pseudokirchneriella subcapitata) biosensor chip for pesticide sensing has been developed by attaching the immobilized microalgae biofilm pon the microarray dye spots (size 100 μm and pitch 200 μm). The dye spots (ruthenium complex) were printed upon SO3-modified glass slides using a polydimethylsiloxane (PDMS) stamp and a microcontact printer (μCP). Emitted fluorescence intensity (FI) variance due to photosynthetic activity (O2 production) of microalgae was monitored by an inverted fluorescent microscope and inhibition of the oxygen generation rate was calculated based on the FI responses both before and after injection of pesticide sample. The calibration curves, as the inhibition of oxygen generation rate (%) due to photosynthetic activity inhibition by the pesticides, depicted that among the 6 tested pesticides, the biosensor showed good sensitivity for 4 pesticides (diuron, simetryn, simazine, and atrazine) but was insensitive for mefenacet and pendimethalin. The detection limits were 1 ppb for diuron and 10 ppb for simetryn, simazine, and atrazine. The simple and low-cost nature of sensing of the developed biosensor sensor chip has apparently created opportunities for regular water quality monitoring, where pesticides are an important concern. View Full-Text
Keywords: microalgae; pesticides; biosensor chip; fluorescence oxygen sensor microalgae; pesticides; biosensor chip; fluorescence oxygen sensor
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MDPI and ACS Style

Kashem, M.A.; Kimoto, K.; Iribe, Y.; Suzuki, M. Development of Microalgae Biosensor Chip by Incorporating Microarray Oxygen Sensor for Pesticides Sensing. Biosensors 2019, 9, 133.

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