Next Article in Journal
The Influence of the Parameters of a Gold Nanoparticle Deposition Method on Titanium Dioxide Nanotubes, Their Electrochemical Response, and Protein Adsorption
Previous Article in Journal
Bioelectrical Analysis of Various Cancer Cell Types Immobilized in 3D Matrix and Cultured in 3D-Printed Well
Previous Article in Special Issue
Development of Microalgae Biosensor Chip by Incorporating Microarray Oxygen Sensor for Pesticides Sensing
Open AccessArticle

Multiwalled Carbon Nanotubes and the Electrocatalytic Activity of Gluconobacter oxydans as the Basis of a Biosensor

1
FSBIS G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia
2
FSBIS V.A. Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Moscow 125009, Russia
3
FSBIS TBP RC, Branch of the FSBIS SRC Institute of Immunology, Federal Immunobiological Agency, Serpukhov 142253, Russia
4
CNR, Institute for Microelectronics and Microsystems, Via Monteroni, 73100 Lecce, Italy
*
Author to whom correspondence should be addressed.
Biosensors 2019, 9(4), 137; https://doi.org/10.3390/bios9040137
Received: 3 October 2019 / Revised: 8 November 2019 / Accepted: 12 November 2019 / Published: 14 November 2019
(This article belongs to the Special Issue Dedication to Professor Isao Karube: Microbial Biosensors)
This paper considers the effect of multiwalled carbon nanotubes (MWCNTs) on the parameters of Gluconobacter oxydans microbial biosensors. MWCNTs were shown not to affect the structural integrity of microbial cells and their respiratory activity. The positive results from using MWCNTs were due to a decrease in the impedance of the electrode. The total impedance of the system decreased significantly, from 9000 kOhm (G. oxydans/chitosan composite) to 600 kOhm (G. oxydans/MWCNTs/chitosan). Modification of the amperometric biosensor with nanotubes led to an increase in the maximal signal from 65 to 869 nA for glucose and from 181 to 1048 nA for ethanol. The biosensor sensitivity also increased 4- and 5-fold, respectively, for each of the substrates. However, the addition of MWCNTs reduced the affinity of respiratory chain enzymes to their substrates (both sugars and alcohols). Moreover, the minimal detection limits were not reduced despite a sensitivity increase. The use of MWCNTs thus improved only some microbial biosensor parameters. View Full-Text
Keywords: microbial electrochemical biosensors; Gluconobacter oxydans; multiwalled carbon nanotubes; impedance spectroscopy; oxygen Clark-type electrode; amperometric detection microbial electrochemical biosensors; Gluconobacter oxydans; multiwalled carbon nanotubes; impedance spectroscopy; oxygen Clark-type electrode; amperometric detection
Show Figures

Figure 1

MDPI and ACS Style

Plekhanova, Y.; Tarasov, S.; Bykov, A.; Prisyazhnaya, N.; Kolesov, V.; Sigaev, V.; Signore, M.A.; Reshetilov, A. Multiwalled Carbon Nanotubes and the Electrocatalytic Activity of Gluconobacter oxydans as the Basis of a Biosensor. Biosensors 2019, 9, 137.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop