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Open AccessArticle

Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera

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Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, Wuhan 430065, China
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Resources and Environmental Engineering College, Wuhan University of Science and Technology, Wuhan 430081, China
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Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, Jianghan University, Wuhan 430056, China
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University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, 69100 Villeurbanne, France
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Authors to whom correspondence should be addressed.
Biosensors 2019, 9(3), 84; https://doi.org/10.3390/bios9030084
Received: 14 June 2019 / Revised: 24 June 2019 / Accepted: 27 June 2019 / Published: 30 June 2019
(This article belongs to the Special Issue Electrochemical Immunosensor)
The electrochemical aptamer sensor has been designed for detecting tau381, a critical biomarker of Alzheimer′s disease in human serum. The aptasensor is obtained by immobilizing the aptamer on a carboxyl graphene/thionin/gold nanoparticle modified glassy-carbon electrode. As a probe and bridge molecule, thionin connected carboxyl graphene and gold nanoparticles, and gave the electrical signal. Under optimal conditions, the increment of differential pulse voltammetry signal increased linearly with the logarithm of tau381 concentration in the range from 1.0 pM to 100 pM, and limit of detection was 0.70 pM. The aptasensor reliability was evaluated by determining its selectivity, reproducibility, stability, detection limit, and recovery. Performance analysis of the tau381 aptasensor in 10 patients’ serum samples showed that the aptasensor could screen patients with and without Alzheimer′s disease. The proposed aptasensor has potential for use in clinically diagnosing Alzheimer′s disease in the early stage. View Full-Text
Keywords: aptasensor; tau381; carboxyl graphene/thionin/gold nanoparticles; human serum; Alzheimer′s disease aptasensor; tau381; carboxyl graphene/thionin/gold nanoparticles; human serum; Alzheimer′s disease
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MDPI and ACS Style

Tao, D.; Shui, B.; Gu, Y.; Cheng, J.; Zhang, W.; Jaffrezic-Renault, N.; Song, S.; Guo, Z. Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera. Biosensors 2019, 9, 84.

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