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Open AccessArticle

Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars

Centre for Human Metabolomics, North-West University, Potchefstroom 2520, South Africa
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Biosensors 2019, 9(3), 83; https://doi.org/10.3390/bios9030083
Received: 3 May 2019 / Revised: 13 June 2019 / Accepted: 24 June 2019 / Published: 29 June 2019
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Abstract

Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sensitivity was observed due to the nanostars clustering after being functionalised with glucose oxidase (GOx). The biosensor quantified glucose in the serum samples with a 1:1000 dilution factor, and colorimetrically distinguished between the concentrations. The assay demonstrated good specificity and sensitivity. The fabricated glucose biosensor is a rapid kinetic assay using a basic entry level laboratory spectrophotometric microplate reader. Such a biosensor could be very useful in resource-constrained regions without state-of-the-art laboratory equipment. Furthermore, naked eye detection of glucose makes this a suitable biosensor for technology transfer to other point-of-care devices. View Full-Text
Keywords: glucose biosensor; gold nanostars; colorimetric detection; glucose oxidase; localized surface plasmon resonance; biocatalytic shape-altering glucose biosensor; gold nanostars; colorimetric detection; glucose oxidase; localized surface plasmon resonance; biocatalytic shape-altering
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Phiri, M.M.; Mulder, D.W.; Vorster, B.C. Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars. Biosensors 2019, 9, 83.

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