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Open AccessArticle

Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate

1
Institute of Genetics, Technische Universität Dresden, Zellescher Weg 20b, 01217 Dresden, Germany
2
Institute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany
*
Author to whom correspondence should be addressed.
Biosensors 2019, 9(3), 104; https://doi.org/10.3390/bios9030104
Received: 12 July 2019 / Revised: 15 August 2019 / Accepted: 26 August 2019 / Published: 29 August 2019
(This article belongs to the Special Issue Functional Nanomaterials for Biosensing and Bioimaging)
Glyphosate, the most widely used pesticide worldwide, is under debate due to its potentially cancerogenic effects and harmful influence on biodiversity and environment. Therefore, the detection of glyphosate in water, food or environmental probes is of high interest. Currently detection of glyphosate usually requires specialized, costly instruments, is labor intensive and time consuming. Here we present a fast and simple method to detect glyphosate in the nanomolar range based on the surface immobilization of glyphosate’s target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) via fusion to the hydrophobin Ccg2 and determination of enzyme activity with a malachite green assay, which is a common photometric technique to measure inorganic phosphate (Pi). The assay demonstrates a new approach for a fast and simple detection of pesticides. View Full-Text
Keywords: glyphosate; malachite green assay; hydrophobin; EPSPS; immobilization glyphosate; malachite green assay; hydrophobin; EPSPS; immobilization
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MDPI and ACS Style

Döring, J.; Rettke, D.; Rödel, G.; Pompe, T.; Ostermann, K. Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate. Biosensors 2019, 9, 104.

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