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Suppressing Non-Specific Binding of Proteins onto Electrode Surfaces in the Development of Electrochemical Immunosensors

Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias. Ave. Eugenio Garza Sada 2501, Monterrey 64849, N.L., Mexico
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Biosensors 2019, 9(1), 15; https://doi.org/10.3390/bios9010015
Received: 15 December 2018 / Revised: 7 January 2019 / Accepted: 13 January 2019 / Published: 18 January 2019
(This article belongs to the Special Issue Biomaterials and Biosensors: Current Advancements)
Electrochemical immunosensors, EIs, are systems that combine the analytical power of electrochemical techniques and the high selectivity and specificity of antibodies in a solid phase immunoassay for target analyte. In EIs, the most used transducer platforms are screen printed electrodes, SPEs. Some characteristics of EIs are their low cost, portability for point of care testing (POCT) applications, high specificity and selectivity to the target molecule, low sample and reagent consumption and easy to use. Despite all these attractive features, still exist one to cover and it is the enhancement of the sensitivity of the EIs. In this review, an approach to understand how this can be achieved is presented. First, it is necessary to comprise thoroughly all the complex phenomena that happen simultaneously in the protein-surface interface when adsorption of the protein occurs. Physicochemical properties of the protein and the surface as well as the adsorption phenomena influence the sensitivity of the EIs. From this point, some strategies to suppress non-specific binding, NSB, of proteins onto electrode surfaces in order to improve the sensitivity of EIs are mentioned. View Full-Text
Keywords: electrochemical immunosensors (EIs); EI sensitivity enhancement; non-specific binding (NSB); protein adsorption electrochemical immunosensors (EIs); EI sensitivity enhancement; non-specific binding (NSB); protein adsorption
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Contreras-Naranjo, J.E.; Aguilar, O. Suppressing Non-Specific Binding of Proteins onto Electrode Surfaces in the Development of Electrochemical Immunosensors. Biosensors 2019, 9, 15.

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