Next Article in Journal
BCAbox Algorithm Expands Capabilities of Raman Microscope for Single Organelles Assessment
Previous Article in Journal
Applications of Nanomaterials for Immunosensing
Article Menu

Export Article

Open AccessArticle
Biosensors 2018, 8(4), 105; https://doi.org/10.3390/bios8040105

PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures

1
Technische Universität Ilmenau, Institute of Micro- and Nanotechnologies, Gustav-Kirchhoff-Straße 7, 98693 Ilmenau, Germany
2
Technische Universität Ilmenau, Department of Nanobiosystem Technology, Institute of Chemistry and Biotechnology, Prof.-Schmidt-Straße 26, 98693 Ilmenau, Germany
*
Authors to whom correspondence should be addressed.
Received: 30 August 2018 / Revised: 30 October 2018 / Accepted: 1 November 2018 / Published: 2 November 2018
Full-Text   |   PDF [5014 KB, uploaded 2 November 2018]   |  

Abstract

Low temperature cofired ceramics (LTCC) provide a technology for the 3-dimensional integration of sensor arrays into bioreactors covering dimensions of several hundred micrometers. Since optical control in such assemblies is not possible, the in situ detection of cell adhesion on impedance electrodes with high spatial resolution would deliver crucial information. A current limitation is the increasing impedance of microelectrodes with decreasing diameter. This study evaluates the suitability of thick film gold electrodes, pristine and coated with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT), for the detection of cell adhesion on the electrode surface. The impedance as criterion for cell attachment is measured with a recording system for electroactive cells with the aim of improving usability. Two cell cultures with different adhesion characteristic are used for adhesion assessment on planar test chips. The impedance increase measured on individual PEDOT coated electrodes due to tight contact of cells reaches a factor of 6.8 in cultures of well-adherent HepG2 cells. Less adhered NG108-15 cells produce a maximum impedance increase by a factor of 2.6. Since the electrode impedance is significantly reduced by PEDOT coating, a reduction of the electrode diameter to values below 100 µm and spatially resolved detection is possible. The results encourage further studies using PEDOT coated thick film electrodes as bio-electronic-interfaces. We presume that such miniaturized electrodes are suitable for 3-dimensional recordings in electroactive cell cultures, providing information of local cell adhesion at the same time. View Full-Text
Keywords: thick film electrodes; LTCC; PEDOT; impedance; spatial resolution; in situ monitoring; cell adhesion; in vitro cell culture; electroactive cells; neuronal cells thick film electrodes; LTCC; PEDOT; impedance; spatial resolution; in situ monitoring; cell adhesion; in vitro cell culture; electroactive cells; neuronal cells
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Bartsch, H.; Peipmann, R.; Klett, M.; Brauer, D.; Schober, A.; Müller, J. PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures. Biosensors 2018, 8, 105.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top