Next Article in Journal
Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture
Next Article in Special Issue
Aptamer-Based Biosensors for Antibiotic Detection: A Review
Previous Article in Journal
Sensors Based on Bio and Biomimetic Receptors in Medical Diagnostic, Environment, and Food Analysis
Previous Article in Special Issue
Review of Stratum Corneum Impedance Measurement in Non-Invasive Penetration Application
Open AccessArticle

The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs

1
Faculdade de Farmácia, Universidade Federal do Goiás (UFG), rua 221 esquina com a 5ª avenida s/n, Setor Universitário, Goiânia-GO 74605-170, Brazil
2
Department of Chemistry, Faculty of Applied Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa
*
Authors to whom correspondence should be addressed.
Biosensors 2018, 8(2), 36; https://doi.org/10.3390/bios8020036
Received: 20 February 2018 / Revised: 12 March 2018 / Accepted: 29 March 2018 / Published: 2 April 2018
The vegetable kingdom is a wide source of a diverse variety of enzymes with broad biotechnological applications. Among the main classes of plant enzymes, the polyphenol oxidases, which convert phenolic compounds to the related quinones, have been successfully used for biosensor development. The oxidation products from such enzymes can be electrochemically reduced, and the sensing is easily achieved by amperometric transducers. In this work, the polyphenoloxidases were extracted from jurubeba (Solanum paniculatum L.) fruits, and the extract was used to construct a carbon paste-based biosensor for pharmaceutical analysis and applications. The assay optimization was performed using a 0.1 mM catechol probe, taking into account the amount of enzymatic extract (50 or 200 μL) and the optimum pH (3.0 to 9.0) as well as some electrochemical differential pulse voltammetric (DPV) parameters (e.g., pulse amplitude, pulse range, pulse width, scan rate). Under optimized conditions, the biosensor was evaluated for the quantitative determination of acetaminophen, acetylsalicylic acid, methyldopa, and ascorbic acid. The best performance was obtained for acetaminophen, which responded linearly in the range between 5 and 245 μM (R = 0.9994), presenting a limit of detection of 3 μM and suitable repeatability ranging between 1.52% and 1.74% relative standard deviation (RSD). View Full-Text
Keywords: plant enzymes; vegetable polyphenoloxidases; amperometric biosensors; pharmaceutical analysis plant enzymes; vegetable polyphenoloxidases; amperometric biosensors; pharmaceutical analysis
Show Figures

Graphical abstract

MDPI and ACS Style

Antunes, R.S.; Garcia, L.F.; Somerset, V.S.; Gil, E.D.S.; Lopes, F.M. The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs. Biosensors 2018, 8, 36.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop