The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs

Antunes, Rafael Souza; Garcia, Luane Ferreira; Somerset, Vernon Sydwill; Gil, Eric De Souza; Lopes, Flavio Marques

doi:10.3390/bios8020036

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The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs

Rafael Souza Antunes

¹,

Luane Ferreira Garcia

¹,

Vernon Sydwill Somerset

Eric De Souza Gil

^1,* and

Flavio Marques Lopes

^1,*

Faculdade de Farmácia, Universidade Federal do Goiás (UFG), rua 221 esquina com a 5ª avenida s/n, Setor Universitário, Goiânia-GO 74605-170, Brazil

Department of Chemistry, Faculty of Applied Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa

Authors to whom correspondence should be addressed.

Biosensors 2018, 8(2), 36; https://doi.org/10.3390/bios8020036

Received: 20 February 2018 / Revised: 12 March 2018 / Accepted: 29 March 2018 / Published: 2 April 2018

(This article belongs to the Special Issue Novel Biosensing Platforms for Disease Diagnosis: Translation of Lab-based Technologies into Clinical Settings)

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Abstract

The vegetable kingdom is a wide source of a diverse variety of enzymes with broad biotechnological applications. Among the main classes of plant enzymes, the polyphenol oxidases, which convert phenolic compounds to the related quinones, have been successfully used for biosensor development. The oxidation products from such enzymes can be electrochemically reduced, and the sensing is easily achieved by amperometric transducers. In this work, the polyphenoloxidases were extracted from jurubeba (Solanum paniculatum L.) fruits, and the extract was used to construct a carbon paste-based biosensor for pharmaceutical analysis and applications. The assay optimization was performed using a 0.1 mM catechol probe, taking into account the amount of enzymatic extract (50 or 200 μL) and the optimum pH (3.0 to 9.0) as well as some electrochemical differential pulse voltammetric (DPV) parameters (e.g., pulse amplitude, pulse range, pulse width, scan rate). Under optimized conditions, the biosensor was evaluated for the quantitative determination of acetaminophen, acetylsalicylic acid, methyldopa, and ascorbic acid. The best performance was obtained for acetaminophen, which responded linearly in the range between 5 and 245 μM (R = 0.9994), presenting a limit of detection of 3 μM and suitable repeatability ranging between 1.52% and 1.74% relative standard deviation (RSD). View Full-Text

Keywords: plant enzymes; vegetable polyphenoloxidases; amperometric biosensors; pharmaceutical analysis

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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MDPI and ACS Style

Antunes, R.S.; Garcia, L.F.; Somerset, V.S.; Gil, E.D.S.; Lopes, F.M. The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs. Biosensors 2018, 8, 36. https://doi.org/10.3390/bios8020036

AMA Style

Antunes RS, Garcia LF, Somerset VS, Gil EDS, Lopes FM. The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs. Biosensors. 2018; 8(2):36. https://doi.org/10.3390/bios8020036

Chicago/Turabian Style

Antunes, Rafael S., Luane F. Garcia, Vernon S. Somerset, Eric D.S. Gil, and Flavio M. Lopes. 2018. "The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs" Biosensors 8, no. 2: 36. https://doi.org/10.3390/bios8020036

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The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs

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