Next Article in Journal
Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques
Previous Article in Journal
A Novel Surface Plasmon Resonance Biosensor for the Rapid Detection of Botulinum Neurotoxins
Previous Article in Special Issue
In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-Effective Thin Gold Film Biosensor
Article Menu
Issue 3 (September) cover image

Export Article

Open AccessArticle
Biosensors 2017, 7(3), 33;

Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment

Department of Basic and Applied Science for Engineering, Sapienza University of Rome, Via A. Scarpa 16, 00161 Rome, Italy
Regina Elena National Cancer Institute, Via E. Chianesi 53, 00144 Rome, Italy
Fraunhofer Institute for Applied Optics and Precision Engineering IOF, Albert-Einstein-Str. 7, 07745 Jena, Germany
Fraunhofer Institute for Material and Beam Technology IWS, Winterbergstr. 28, 01277 Dresden, Germany
IBI-Istituto Biochimico Italiano Giovanni Lorenzini Spa, Via Fossignano 2, 04011 Aprilia, Italy
Present Address: Bambino Gesù Pediatric Hospital, Viale S. Paolo 15, 00146 Rome, Italy.
Author to whom correspondence should be addressed.
Received: 27 June 2017 / Revised: 11 August 2017 / Accepted: 15 August 2017 / Published: 17 August 2017
(This article belongs to the Special Issue Biosensors for the Detection of Biomarkers)
Full-Text   |   PDF [3227 KB, uploaded 17 August 2017]   |  


We report on the use of one-dimensional photonic crystals to detect clinically relevant concentrations of the cancer biomarker ERBB2 in cell lysates. Overexpression of the ERBB2 protein is associated with aggressive breast cancer subtypes. To detect soluble ERBB2, we developed an optical set-up which operates in both label-free and fluorescence modes. The detection approach makes use of a sandwich assay, in which the one-dimensional photonic crystals sustaining Bloch surface waves are modified with monoclonal antibodies, in order to guarantee high specificity during the biological recognition. We present the results of exemplary protein G based label-free assays in complex biological matrices, reaching an estimated limit of detection of 0.5 ng/mL. On-chip and chip-to-chip variability of the results is addressed too, providing repeatability rates. Moreover, results on fluorescence operation demonstrate the capability to perform high sensitive cancer biomarker assays reaching a resolution of 0.6 ng/mL, without protein G assistance. The resolution obtained in both modes meets international guidelines and recommendations (15 ng/mL) for ERBB2 quantification assays, providing an alternative tool to phenotype and diagnose molecular cancer subtypes. View Full-Text
Keywords: Optical biosensors; Bloch surface waves; 1D photonic crystals; ERBB2; SK-BR 3; Breast cancer Optical biosensors; Bloch surface waves; 1D photonic crystals; ERBB2; SK-BR 3; Breast cancer

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Sinibaldi, A.; Sampaoli, C.; Danz, N.; Munzert, P.; Sonntag, F.; Centola, F.; Occhicone, A.; Tremante, E.; Giacomini, P.; Michelotti, F. Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment. Biosensors 2017, 7, 33.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top