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High Content Imaging (HCI) on Miniaturized Three-Dimensional (3D) Cell Cultures
Open AccessArticle

Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules

Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires—Institut de Chimie et Biochimie Moléculaires et Supramoléculaires—Université Claude Bernard Lyon 1, Villeurbanne 69100, France
Author to whom correspondence should be addressed.
Academic Editor: Roberto Pilloton
Biosensors 2016, 6(1), 2;
Received: 8 December 2015 / Revised: 8 January 2016 / Accepted: 12 January 2016 / Published: 15 January 2016
(This article belongs to the Special Issue Cell and Organ on Chip: Challenges and Advances)
PDF [2577 KB, uploaded 15 January 2016]


In the present report, we are making the proof of concept of cell small populations (from 1 to 100 cells) spotting, culture and secretion detection on a gold surface. In order to keep the cells in a hydrated environment during the robotized micropipetting and to address different cell lines on a single chip, a biocompatible alginate polymer was used. This approach enables the encapsulation of the cell in a very small volume (30 nL), directly on the substrate and permits a precise control of the number of cells in each alginate bead. After 24 h of culture, the adherent cells are ready for surface plasmon resonance imaging (SPRi) experimentation. To enable the detection of secreted proteins, various antibodies are immobilized in an organized manner on a SPRi sensor and permitted the multiplex detection of different proteins secreted by the different cultured cell lines. Evidence of the real-time detection will be presented for Prostate Specific Antigen (PSA) and β-2-microglobulin (B2M) secreted by prostate cancer cells following induction by dihydrotestosterone (DHT). Different kinetics for the two secreted proteins were then demonstrated and precisely determined using the chip. View Full-Text
Keywords: biosensor; cell microarray; cell encapsulation; SPRi; real-time biosensor; cell microarray; cell encapsulation; SPRi; real-time

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Berthuy, O.I.; Blum, L.J.; Marquette, C.A. Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules. Biosensors 2016, 6, 2.

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