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Biosensors 2015, 5(4), 768-790;

High Content Imaging (HCI) on Miniaturized Three-Dimensional (3D) Cell Cultures

Department of Chemical & Biomedical Engineering, Cleveland State University, 1960 East 24th Street Cleveland, Ohio, OH 44115-2214, USA
Author to whom correspondence should be addressed.
Academic Editor: Christophe A. Marquette
Received: 26 September 2015 / Revised: 9 December 2015 / Accepted: 10 December 2015 / Published: 14 December 2015
(This article belongs to the Special Issue Cell and Organ on Chip: Challenges and Advances)
Full-Text   |   PDF [572 KB, uploaded 14 December 2015]   |  


High content imaging (HCI) is a multiplexed cell staining assay developed for better understanding of complex biological functions and mechanisms of drug action, and it has become an important tool for toxicity and efficacy screening of drug candidates. Conventional HCI assays have been carried out on two-dimensional (2D) cell monolayer cultures, which in turn limit predictability of drug toxicity/efficacy in vivo; thus, there has been an urgent need to perform HCI assays on three-dimensional (3D) cell cultures. Although 3D cell cultures better mimic in vivo microenvironments of human tissues and provide an in-depth understanding of the morphological and functional features of tissues, they are also limited by having relatively low throughput and thus are not amenable to high-throughput screening (HTS). One attempt of making 3D cell culture amenable for HTS is to utilize miniaturized cell culture platforms. This review aims to highlight miniaturized 3D cell culture platforms compatible with current HCI technology. View Full-Text
Keywords: high content imaging; three-dimensional (3D) cell culture; miniaturized cell-based assay; predictive toxicology high content imaging; three-dimensional (3D) cell culture; miniaturized cell-based assay; predictive toxicology

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Joshi, P.; Lee, M.-Y. High Content Imaging (HCI) on Miniaturized Three-Dimensional (3D) Cell Cultures. Biosensors 2015, 5, 768-790.

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