Next Article in Journal / Special Issue
High Content Imaging (HCI) on Miniaturized Three-Dimensional (3D) Cell Cultures
Previous Article in Journal / Special Issue
Microfluidic Impedimetric Cell Regeneration Assay to Monitor the Enhanced Cytotoxic Effect of Nanomaterial Perfusion
Open AccessArticle

A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip

1
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
2
CEA, INAC-SPrAM, Grenoble F-38000, France
3
CNRS, INAC-SPrAM, Grenoble F-38000, France
4
University Grenoble Alpes, Institut Albert Bonniot, Grenoble F-38000, France
5
INSERM U823, Grenoble F-38000, France
*
Author to whom correspondence should be addressed.
Academic Editor: Christophe A. Marquette
Biosensors 2015, 5(4), 750-767; https://doi.org/10.3390/bios5040750
Received: 30 September 2015 / Revised: 12 November 2015 / Accepted: 25 November 2015 / Published: 4 December 2015
(This article belongs to the Special Issue Cell and Organ on Chip: Challenges and Advances)
The identification and characterization, at the cellular level, of cytokine productions present a high interest for both fundamental research and clinical studies. However, the majority of techniques currently available (ELISA, ELISpot, flow cytometry, etc.) have several shortcomings including, notably, the assessment of several cytokines in relation to individual secreting cells and the monitoring of living cell responses for a long incubation time. In the present work, we describe a system composed of a microfluidic platform coupled with an antibody microarray chip for continuous SPR imaging and immunofluorescence analysis of cytokines (IL-2 and IFN-γ) secreted by T-Lymphocytes, specifically, and stably captured on the biochip under flow upon continued long-term on-chip culture (more than 24 h). View Full-Text
Keywords: Cytokine; T-Lymphocytes; biochip; microfluidics; antibody; PDMS; self-assembled monolayers; SPR imaging Cytokine; T-Lymphocytes; biochip; microfluidics; antibody; PDMS; self-assembled monolayers; SPR imaging
Show Figures

Figure 1

MDPI and ACS Style

Baganizi, D.R.; Leroy, L.; Laplatine, L.; Fairley, S.J.; Heidmann, S.; Menad, S.; Livache, T.; Marche, P.N.; Roupioz, Y. A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip. Biosensors 2015, 5, 750-767.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop