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Open AccessArticle

Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion

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Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France
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Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR8576 du CNRS, Université Lille 1, 59655 Villeneuve d'Ascq, France
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Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine
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SENSIA SL, Poligono Aranguren, 9, Apdo. Correos 171, 20180 Oiartzun, Gipuzkoa, Spain
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LUNAM Université, CEISAM, UMR 6230 du CNRS, 2, rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France
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Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gheorghe Polizu, 011061 Bucharest, Romania
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National R&D Institute for Chemistry and Petrochemistry ICECHIM, 202 Splaiul Independentei, 060021 Bucharest, Romania
*
Authors to whom correspondence should be addressed.
Academic Editors: Nicole Jaffrezic-Renault and Carole Chaix
Biosensors 2015, 5(2), 276-287; https://doi.org/10.3390/bios5020276
Received: 30 April 2015 / Accepted: 19 May 2015 / Published: 26 May 2015
(This article belongs to the Special Issue Affinity Sensors)
The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min−1), and reaches a maximum at flow rates of 30 µL·min−1 (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces. View Full-Text
Keywords: surface plasmon resonance (SPR); shear force enhancement; flow rate; Escherichia coli (E. coli); carbohydrates surface plasmon resonance (SPR); shear force enhancement; flow rate; Escherichia coli (E. coli); carbohydrates
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Zagorodko, O.; Bouckaert, J.; Dumych, T.; Bilyy, R.; Larroulet, I.; Serrano, A.Y.; Dorta, D.A.; Gouin, S.G.; Dima, S.-O.; Oancea, F.; Boukherroub, R.; Szunerits, S. Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion. Biosensors 2015, 5, 276-287.

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