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Biosensors 2015, 5(2), 276-287;

Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion

Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR8576 du CNRS, Université Lille 1, 59655 Villeneuve d'Ascq, France
Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine
SENSIA SL, Poligono Aranguren, 9, Apdo. Correos 171, 20180 Oiartzun, Gipuzkoa, Spain
LUNAM Université, CEISAM, UMR 6230 du CNRS, 2, rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France
Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gheorghe Polizu, 011061 Bucharest, Romania
National R&D Institute for Chemistry and Petrochemistry ICECHIM, 202 Splaiul Independentei, 060021 Bucharest, Romania
Authors to whom correspondence should be addressed.
Academic Editors: Nicole Jaffrezic-Renault and Carole Chaix
Received: 30 April 2015 / Accepted: 19 May 2015 / Published: 26 May 2015
(This article belongs to the Special Issue Affinity Sensors)
Full-Text   |   PDF [521 KB, uploaded 26 May 2015]   |  


The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min−1), and reaches a maximum at flow rates of 30 µL·min−1 (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces. View Full-Text
Keywords: surface plasmon resonance (SPR); shear force enhancement; flow rate; Escherichia coli (E. coli); carbohydrates surface plasmon resonance (SPR); shear force enhancement; flow rate; Escherichia coli (E. coli); carbohydrates

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Zagorodko, O.; Bouckaert, J.; Dumych, T.; Bilyy, R.; Larroulet, I.; Serrano, A.Y.; Dorta, D.A.; Gouin, S.G.; Dima, S.-O.; Oancea, F.; Boukherroub, R.; Szunerits, S. Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion. Biosensors 2015, 5, 276-287.

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