Next Article in Journal
CNN-Based Classification of Ziziphus Seeds with Focal Loss for Overcoming Size-Based Shortcut Learning
Previous Article in Journal
Development of Laser-Scribed Graphene Electrodes for Label-Free L-Histidine Sensing in Artificial Sweat
Previous Article in Special Issue
A RPA-CRISPR/Cas12a-Powered Catalytic Hairpin Assembly Fluorescence Biosensor for Duck Plague Virus Virulent Strain Detection
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biosensors
Volume 16
Issue 6
10.3390/bios16060321
biosensors-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

A Centrifugal Microfluidic Platform Integrating Immunomagnetic Separation and Isothermal Amplification for Rapid and High-Sensitivity Detection of Foodborne Pathogens

by
Qingfeng Zheng
1,2,
Zhun Zhuang
1,2,
Hua Lei
1,2,
Jianhan Lin
1,2,*,
Hua Yang
1,3,* and
Ruibin Hu
1,*
1
Institute of Biotechnology, Xianghu Laboratory, Hangzhou 311231, China
2
College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China
3
Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
*
Authors to whom correspondence should be addressed.
Biosensors 2026, 16(6), 321; https://doi.org/10.3390/bios16060321
Submission received: 29 April 2026 / Revised: 22 May 2026 / Accepted: 29 May 2026 / Published: 2 June 2026
(This article belongs to the Special Issue Sensors for Environmental Monitoring and Food Safety—2nd Edition)
Browse Figures
Versions Notes

Abstract

The contamination by foodborne pathogens posed a significant health threat and huge economic burden. Traditional detection methods were limited by cumbersome and time-consuming procedures, low automation, and reliance on expensive instrumentation, making them inadequate for on-site detection. This paper presented a centrifugal microfluidic chip that integrated sample pretreatment, nucleic acid extraction, amplification reaction, and signal detection. The chip featured an innovative design that combined a bursting valve with the siphon channel and employed a dual-channel configuration for splitting and directing the flow of different reagents, thereby overcoming the instability issue of unintended pre-activation or interruption that often occurred in the cascade design of multilevel siphon channels. Moreover, by synergistically combining with immunomagnetic separation as well as multi-enzyme isothermal rapid amplification, a portable, easy, rapid, high-sensitivity, and low-cost point-of-care testing (POCT) system for foodborne pathogens was developed. Under optimized conditions, the system enabled detection of Salmonella in spiked milk samples at 10 CFU/mL in 1 h. The recoveries ranged from 83.22% to 127.60%, with relative standard deviations of ≤13.7%, indicating that this system had great potential for rapid and high-sensitivity detection of foodborne pathogens in resource-limited settings.
Keywords: foodborne pathogens; immunomagnetic separation; multienzyme isothermal rapid amplification; centrifugal microfluidic chip; point-of-care testing foodborne pathogens; immunomagnetic separation; multienzyme isothermal rapid amplification; centrifugal microfluidic chip; point-of-care testing
Graphical Abstract

Share and Cite

MDPI and ACS Style

Zheng, Q.; Zhuang, Z.; Lei, H.; Lin, J.; Yang, H.; Hu, R. A Centrifugal Microfluidic Platform Integrating Immunomagnetic Separation and Isothermal Amplification for Rapid and High-Sensitivity Detection of Foodborne Pathogens. Biosensors 2026, 16, 321. https://doi.org/10.3390/bios16060321

AMA Style

Zheng Q, Zhuang Z, Lei H, Lin J, Yang H, Hu R. A Centrifugal Microfluidic Platform Integrating Immunomagnetic Separation and Isothermal Amplification for Rapid and High-Sensitivity Detection of Foodborne Pathogens. Biosensors. 2026; 16(6):321. https://doi.org/10.3390/bios16060321

Chicago/Turabian Style

Zheng, Qingfeng, Zhun Zhuang, Hua Lei, Jianhan Lin, Hua Yang, and Ruibin Hu. 2026. "A Centrifugal Microfluidic Platform Integrating Immunomagnetic Separation and Isothermal Amplification for Rapid and High-Sensitivity Detection of Foodborne Pathogens" Biosensors 16, no. 6: 321. https://doi.org/10.3390/bios16060321

APA Style

Zheng, Q., Zhuang, Z., Lei, H., Lin, J., Yang, H., & Hu, R. (2026). A Centrifugal Microfluidic Platform Integrating Immunomagnetic Separation and Isothermal Amplification for Rapid and High-Sensitivity Detection of Foodborne Pathogens. Biosensors, 16(6), 321. https://doi.org/10.3390/bios16060321

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop