Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme
Abstract
:1. Introduction
2. Experimental Methods
2.1. Materials and Characterization
2.2. Preparation of the Photonic Hydrogel Aptasensors
2.3. Measurement of Debye Diffraction Ring
3. Results and Discussion
3.1. Preparation and Characterization of the Photonic Hydrogel Aptasensors
3.2. Response Mechanism of the Aptasensor
3.3. Response Performance of DNA−H1−960 toward Lysozyme
3.4. Detection of Lysozyme in Human Serum
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample # | Total (g) | AAm (g) | AAc (g) | Bis (g) | TBAm (g) | H2O (g) |
---|---|---|---|---|---|---|
H1 | 15 | 1.20 | 0.45 | 0.045 | 0.16 | 13.15 |
H2 | -- | 13.31 |
Sample | Added (nM) | Found (nM) | Recovery % | RSD % (n = 3) |
---|---|---|---|---|
Human serum | 20 | 21.37 | 106.90 | 0.64 |
50 | 49.97 | 99.94 | 0.76 | |
80 | 82.96 | 103.70 | 1.05 |
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Shen, P.; Shi, Y.; Li, R.; Han, B.; Ma, H.; Hou, X.; Zhang, Y.; Jiang, L. Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme. Biosensors 2022, 12, 662. https://doi.org/10.3390/bios12080662
Shen P, Shi Y, Li R, Han B, Ma H, Hou X, Zhang Y, Jiang L. Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme. Biosensors. 2022; 12(8):662. https://doi.org/10.3390/bios12080662
Chicago/Turabian StyleShen, Peiyan, Yuqing Shi, Ran Li, Bo Han, Haojie Ma, Xueyan Hou, Yuqi Zhang, and Lei Jiang. 2022. "Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme" Biosensors 12, no. 8: 662. https://doi.org/10.3390/bios12080662