Next Article in Journal
Technology for Rapid Detection of Cyromazine Residues in Fruits and Vegetables: Molecularly Imprinted Electrochemical Sensors
Next Article in Special Issue
Strategies for Enhancing the Sensitivity of Electrochemiluminescence Biosensors
Previous Article in Journal
Two Biosensors for the Determination of Interleukin-6 in Blood Plasma by Array SPRi
Previous Article in Special Issue
Rapid Capturing and Chemiluminescent Sensing of Programmed Death Ligand-1 Expressing Extracellular Vesicles
 
 
Article

ImmunoDisk—A Fully Automated Bead-Based Immunoassay Cartridge with All Reagents Pre-Stored

1
Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
2
Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
3
BioVendor-Laboratorní Medicína a.s., Research & Diagnostic Products Division, Karasek 1767/1, Reckovice, 62100 Brno, Czech Republic
4
Faculty of Pharmacy, Masaryk University, Palackeho trida 1946/1, 61242 Brno, Czech Republic
5
Section of Oral Health and Periodontology, Division of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, 14104 Huddinge, Sweden
*
Authors to whom correspondence should be addressed.
Biosensors 2022, 12(6), 413; https://doi.org/10.3390/bios12060413
Received: 13 May 2022 / Revised: 31 May 2022 / Accepted: 7 June 2022 / Published: 14 June 2022
(This article belongs to the Special Issue Bioassays and Biosensors for Rapid Detection and Analysis)
In this paper, we present the ImmunoDisk, a fully automated sample-to-answer centrifugal microfluidic cartridge, integrating a heterogeneous, wash-free, magnetic- and fluorescent bead-based immunoassay (bound-free phase detection immunoassay/BFPD-IA). The BFPD-IA allows the implementation of a simple fluidic structure, where the assay incubation, bead separation and detection are performed in the same chamber. The system was characterized using a C-reactive protein (CRP) competitive immunoassay. A parametric investigation on air drying of protein-coupled beads for pre-storage at room temperature is presented. The key parameters were buffer composition, drying temperature and duration. A protocol for drying two different types of protein-coupled beads with the same temperature and duration using different drying buffers is presented. The sample-to-answer workflow was demonstrated measuring CRP in 5 µL of human serum, without prior dilution, utilizing only one incubation step, in 20 min turnaround time, in the clinically relevant concentration range of 15–115 mg/L. A reproducibility assessment over three disk batches revealed an average signal coefficient of variation (CV) of 5.8 ± 1.3%. A CRP certified reference material was used for method verification with a concentration CV of 8.6%. Our results encourage future testing of the CRP-ImmunoDisk in clinical studies and its point-of-care implementation in many diagnostic applications. View Full-Text
Keywords: immunoassay; bound-free phase; micro/nanoparticles; point-of-care; centrifugal microfluidics; inflammation; reagent storage immunoassay; bound-free phase; micro/nanoparticles; point-of-care; centrifugal microfluidics; inflammation; reagent storage
Show Figures

Graphical abstract

MDPI and ACS Style

Johannsen, B.; Baumgartner, D.; Karkossa, L.; Paust, N.; Karpíšek, M.; Bostanci, N.; Zengerle, R.; Mitsakakis, K. ImmunoDisk—A Fully Automated Bead-Based Immunoassay Cartridge with All Reagents Pre-Stored. Biosensors 2022, 12, 413. https://doi.org/10.3390/bios12060413

AMA Style

Johannsen B, Baumgartner D, Karkossa L, Paust N, Karpíšek M, Bostanci N, Zengerle R, Mitsakakis K. ImmunoDisk—A Fully Automated Bead-Based Immunoassay Cartridge with All Reagents Pre-Stored. Biosensors. 2022; 12(6):413. https://doi.org/10.3390/bios12060413

Chicago/Turabian Style

Johannsen, Benita, Desirée Baumgartner, Lena Karkossa, Nils Paust, Michal Karpíšek, Nagihan Bostanci, Roland Zengerle, and Konstantinos Mitsakakis. 2022. "ImmunoDisk—A Fully Automated Bead-Based Immunoassay Cartridge with All Reagents Pre-Stored" Biosensors 12, no. 6: 413. https://doi.org/10.3390/bios12060413

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop