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Volume 12
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Article

Accurate and Rapid Genetic Tracing the Authenticity of Floral Originated Honey with the Molecular Lateral Flow Strip

by
Qian Wu
1,†,
Qi Chen
1,†,
Chao Yan
1,2,
Jianguo Xu
1,
Zhaoran Chen
2,
Li Yao
1,
Jianfeng Lu
1,
Bangben Yao
2,* and
Wei Chen
1,3,*
1
Engineering Research Center of Bio-Process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China
2
Product Quality Supervision and Inspection Research Institute of Anhui Province, Hefei 230009, China
3
Intelligent Manufacturing Institute, Hefei University of Technology, Hefei 230009, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biosensors 2022, 12(11), 971; https://doi.org/10.3390/bios12110971
Submission received: 18 September 2022 / Revised: 14 October 2022 / Accepted: 24 October 2022 / Published: 4 November 2022
(This article belongs to the Special Issue Application of Biosensors in Food Safety Analysis)
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Abstract

Honey is a natural product and is heavily consumed for its well-known nutritional functions. Honeys with different floral origins possess distinctive flavors, tastes, functions and economic values. It is vital to establish an effective strategy for identifying the authenticity of honey. The intrinsic genetic materials of pollen were adopted as target analytes for the effective identification of honey with floral origins. With an optimized protocol for the rapid gene extraction from honey, target genetic templates were amplified on-site with a portable device. Conveniently, all on-site amplified functional products were easily judged by the designed lateral flow strip (LFS), which was defined as the molecular LFS in this research. Additionally, the entire on-site genetic authentication of honey was completed in less than 2 h by visual observation. Commercial honey products have been successfully identified with excellent accuracy. This low-cost, high-efficiency and easy-operational strategy will greatly benefit the quality guarantee of foods with specific functions and geographical markers.
Keywords: locust honey; authentication; molecular amplification; lateral flow strip; food safety locust honey; authentication; molecular amplification; lateral flow strip; food safety

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MDPI and ACS Style

Wu, Q.; Chen, Q.; Yan, C.; Xu, J.; Chen, Z.; Yao, L.; Lu, J.; Yao, B.; Chen, W. Accurate and Rapid Genetic Tracing the Authenticity of Floral Originated Honey with the Molecular Lateral Flow Strip. Biosensors 2022, 12, 971. https://doi.org/10.3390/bios12110971

AMA Style

Wu Q, Chen Q, Yan C, Xu J, Chen Z, Yao L, Lu J, Yao B, Chen W. Accurate and Rapid Genetic Tracing the Authenticity of Floral Originated Honey with the Molecular Lateral Flow Strip. Biosensors. 2022; 12(11):971. https://doi.org/10.3390/bios12110971

Chicago/Turabian Style

Wu, Qian, Qi Chen, Chao Yan, Jianguo Xu, Zhaoran Chen, Li Yao, Jianfeng Lu, Bangben Yao, and Wei Chen. 2022. "Accurate and Rapid Genetic Tracing the Authenticity of Floral Originated Honey with the Molecular Lateral Flow Strip" Biosensors 12, no. 11: 971. https://doi.org/10.3390/bios12110971

APA Style

Wu, Q., Chen, Q., Yan, C., Xu, J., Chen, Z., Yao, L., Lu, J., Yao, B., & Chen, W. (2022). Accurate and Rapid Genetic Tracing the Authenticity of Floral Originated Honey with the Molecular Lateral Flow Strip. Biosensors, 12(11), 971. https://doi.org/10.3390/bios12110971

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