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Article

Highly Sensitive and Specific SARS-CoV-2 Serological Assay Using a Magnetic Modulation Biosensing System

1
Faculty of Engineering, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan 5290002, Israel
2
Central Virology Laboratory, Israel Ministry of Health, Chaim Sheba Medical Center, Tel-HaShomer, Ramat Gan 5262000, Israel
3
Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel
4
The Center for Geographic Medicine, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan 5262000, Israel
*
Author to whom correspondence should be addressed.
Biosensors 2022, 12(1), 7; https://doi.org/10.3390/bios12010007
Received: 9 November 2021 / Revised: 5 December 2021 / Accepted: 8 December 2021 / Published: 23 December 2021
(This article belongs to the Special Issue Biosensors for Body Fluid Analysis)
Sensitive serological assays are needed to provide valuable information about acute and past viral infections. For example, detection of anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG antibodies could serve as the basis for an “immunity passport” that would enable individuals to travel internationally. Here, utilizing a novel Magnetic Modulation Biosensing (MMB) system and the receptor-binding domain of the SARS-CoV-2 spike protein, we demonstrate a highly sensitive and specific anti-SARS-CoV-2 IgG serological assay. Using anti-SARS-CoV-2 IgG antibodies, RT-qPCR SARS-CoV-2-positive and healthy patients’ samples, and vaccinees’ samples, we compare the MMB-based SARS-CoV-2 IgG assay’s analytical and clinical sensitivities to those of the enzyme-linked immunosorbent assay (ELISA). Compared with ELISA, the MMB-based assay has an ~6-fold lower limit of detection (129 ng/L vs. 817 ng/L), and it detects an increase in the IgG concentration much earlier after vaccination. Using 85 RT-qPCR SARS-CoV-2-positive samples and 79 -negative samples, the MMB-based assay demonstrated similar clinical specificity (98% vs. 99%) and sensitivity (93% vs. 92%) to the ELISA test, but with a much faster turnaround time (45 min vs. 245 min). The high analytical and clinical sensitivity, short turnaround time, and simplicity of the MMB-based assay makes it a preferred method for antibody detection. View Full-Text
Keywords: SARS-CoV-2; COVID-19; magnetic modulation; optical biosensing; serology; fluorescence-based assay SARS-CoV-2; COVID-19; magnetic modulation; optical biosensing; serology; fluorescence-based assay
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MDPI and ACS Style

Avivi-Mintz, S.; Lustig, Y.; Indenbaum, V.; Schwartz, E.; Danielli, A. Highly Sensitive and Specific SARS-CoV-2 Serological Assay Using a Magnetic Modulation Biosensing System. Biosensors 2022, 12, 7. https://doi.org/10.3390/bios12010007

AMA Style

Avivi-Mintz S, Lustig Y, Indenbaum V, Schwartz E, Danielli A. Highly Sensitive and Specific SARS-CoV-2 Serological Assay Using a Magnetic Modulation Biosensing System. Biosensors. 2022; 12(1):7. https://doi.org/10.3390/bios12010007

Chicago/Turabian Style

Avivi-Mintz, Shira, Yaniv Lustig, Victoria Indenbaum, Eli Schwartz, and Amos Danielli. 2022. "Highly Sensitive and Specific SARS-CoV-2 Serological Assay Using a Magnetic Modulation Biosensing System" Biosensors 12, no. 1: 7. https://doi.org/10.3390/bios12010007

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