Next Article in Journal
Ratiometric G-Quadruplex Assay for Robust Lead Detection in Food Samples
Previous Article in Journal
Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of Saccharomyces pastorianus from Dekkera spp.
Previous Article in Special Issue
Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads
Article

Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application

1
School of Chemical and Biomolecular Sciences, Southern Illinois University, 1245 Lincoln Drive, Carbondale, IL 62918, USA
2
School of Physics and Applied Physics, Southern Illinois University, Carbondale, IL 62918, USA
*
Author to whom correspondence should be addressed.
Biosensors 2021, 11(8), 273; https://doi.org/10.3390/bios11080273
Received: 26 July 2021 / Revised: 12 August 2021 / Accepted: 12 August 2021 / Published: 14 August 2021
(This article belongs to the Special Issue Microfluidic Bio-Sensors and Their Applications)
DNA is strongly adsorbed on oxidized graphene surfaces in the presence of divalent cations. Here, we studied the effect of DNA adsorption on electrochemical charge transfer at few-layered, oxygen-functionalized graphene (GOx) electrodes. DNA adsorption on the inkjet-printed GOx electrodes caused amplified current response from ferro/ferricyanide redox probe at concentration range 1 aM–10 nM in differential pulse voltammetry. We studied a number of variables that may affect the current response of the interface: sequence type, conformation, concentration, length, and ionic strength. Later, we showed a proof-of-concept DNA biosensing application, which is free from chemical immobilization of the probe and sensitive at attomolar concentration regime. We propose that GOx electrodes promise a low-cost solution to fabricate a highly sensitive platform for label-free and chemisorption-free DNA biosensing. View Full-Text
Keywords: DNA biosensors; graphene electrodes; inkjet-printing; trinucleotide repeats; label-free; electrochemical biosensors DNA biosensors; graphene electrodes; inkjet-printing; trinucleotide repeats; label-free; electrochemical biosensors
Show Figures

Figure 1

MDPI and ACS Style

Asefifeyzabadi, N.; Holland, T.E.; Sivakumar, P.; Talapatra, S.; Senanayake, I.M.; Goodson, B.M.; Shamsi, M.H. Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application. Biosensors 2021, 11, 273. https://doi.org/10.3390/bios11080273

AMA Style

Asefifeyzabadi N, Holland TE, Sivakumar P, Talapatra S, Senanayake IM, Goodson BM, Shamsi MH. Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application. Biosensors. 2021; 11(8):273. https://doi.org/10.3390/bios11080273

Chicago/Turabian Style

Asefifeyzabadi, Narges, Torrey E. Holland, Poopalasingam Sivakumar, Saikat Talapatra, Ishani M. Senanayake, Boyd M. Goodson, and Mohtashim H. Shamsi 2021. "Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application" Biosensors 11, no. 8: 273. https://doi.org/10.3390/bios11080273

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop