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Open AccessEditor’s ChoiceArticle

Microfluidic-Based Electrochemical Immunosensing of Ferritin

1
CSIR-Central Scientific Instruments Organisation, Sector 30-C, Chandigarh 160030, India
2
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India
3
Department of Chemistry and Biochemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UK
*
Authors to whom correspondence should be addressed.
Biosensors 2020, 10(8), 91; https://doi.org/10.3390/bios10080091
Received: 9 July 2020 / Revised: 1 August 2020 / Accepted: 2 August 2020 / Published: 5 August 2020
(This article belongs to the Special Issue Microfluidics for Biosensing)
Ferritin is a clinically important biomarker which reflects the state of iron in the body and is directly involved with anemia. Current methods available for ferritin estimation are generally not portable or they do not provide a fast response. To combat these issues, an attempt was made for lab-on-a-chip-based electrochemical detection of ferritin, developed with an integrated electrochemically active screen-printed electrode (SPE), combining nanotechnology, microfluidics, and electrochemistry. The SPE surface was modified with amine-functionalized graphene oxide to facilitate the binding of ferritin antibodies on the electrode surface. The functionalized SPE was embedded in the microfluidic flow cell with a simple magnetic clamping mechanism to allow continuous electrochemical detection of ferritin. Ferritin detection was accomplished via cyclic voltammetry with a dynamic linear range from 7.81 to 500 ng·mL−1 and an LOD of 0.413 ng·mL−1. The sensor performance was verified with spiked human serum samples. Furthermore, the sensor was validated by comparing its response with the response of the conventional ELISA method. The current method of microfluidic flow cell-based electrochemical ferritin detection demonstrated promising sensitivity and selectivity. This confirmed the plausibility of using the reported technique in point-of-care testing applications at a much faster rate than conventional techniques. View Full-Text
Keywords: amine functionalization; graphene oxide; immunosensor; electrochemistry; ferritin; microfluidics amine functionalization; graphene oxide; immunosensor; electrochemistry; ferritin; microfluidics
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MDPI and ACS Style

Garg, M.; Christensen, M.G.; Iles, A.; Sharma, A.L.; Singh, S.; Pamme, N. Microfluidic-Based Electrochemical Immunosensing of Ferritin. Biosensors 2020, 10, 91.

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