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Article

Towards Bacteria Counting in DI Water of Several Microliters or Growing Suspension Using Impedance Biochips

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Department Nano Device Technology, Fraunhofer Institute for Electronic Nano Systems, Technologie-Campus 3, 09126 Chemnitz, Germany
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Material Systems for Nanoelectronics, Chemnitz University of Technology, 09126 Chemnitz, Germany
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Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany
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Center for Sepsis Control and Care, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany
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Institute for Solid State Physics, Friedrich Schiller University Jena, 07743 Jena, Germany
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Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstraße 400, 01328 Dresden, Germany
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Institute for Integrative Nanosciences IFW Dresden, Helmholtzstr. 20, 01069 Dresden, Germany
*
Authors to whom correspondence should be addressed.
Biosensors 2020, 10(8), 82; https://doi.org/10.3390/bios10080082
Received: 6 June 2020 / Revised: 9 July 2020 / Accepted: 18 July 2020 / Published: 23 July 2020
(This article belongs to the Section Biosensor and Bioelectronic Devices)
We counted bacterial cells of E. coli strain K12 in several-microliter DI water or in several-microliter PBS in the low optical density (OD) range (OD = 0.05–1.08) in contact with the surface of Si-based impedance biochips with ring electrodes by impedance measurements. The multiparameter fit of the impedance data allowed calibration of the impedance data with the concentration cb of the E. coli cells in the range of cb = 0.06 to 1.26 × 109 cells/mL. The results showed that for E. coli in DI water and in PBS, the modelled impedance parameters depend linearly on the concentration of cells in the range of cb = 0.06 to 1.26 × 109 cells/mL, whereas the OD, which was independently measured with a spectrophotometer, was only linearly dependent on the concentration of the E. coli cells in the range of cb = 0.06 to 0.50 × 109 cells/mL. View Full-Text
Keywords: bacterial cell counting; biochips; impedance spectroscopy; Escherichia coli; electrical equivalent circuit model bacterial cell counting; biochips; impedance spectroscopy; Escherichia coli; electrical equivalent circuit model
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MDPI and ACS Style

Kiani, M.; Tannert, A.; Du, N.; Hübner, U.; Skorupa, I.; Bürger, D.; Zhao, X.; Blaschke, D.; Rebohle, L.; Cherkouk, C.; Neugebauer, U.; Schmidt, O.G.; Schmidt, H. Towards Bacteria Counting in DI Water of Several Microliters or Growing Suspension Using Impedance Biochips. Biosensors 2020, 10, 82. https://doi.org/10.3390/bios10080082

AMA Style

Kiani M, Tannert A, Du N, Hübner U, Skorupa I, Bürger D, Zhao X, Blaschke D, Rebohle L, Cherkouk C, Neugebauer U, Schmidt OG, Schmidt H. Towards Bacteria Counting in DI Water of Several Microliters or Growing Suspension Using Impedance Biochips. Biosensors. 2020; 10(8):82. https://doi.org/10.3390/bios10080082

Chicago/Turabian Style

Kiani, Mahdi; Tannert, Astrid; Du, Nan; Hübner, Uwe; Skorupa, Ilona; Bürger, Danilo; Zhao, Xianyue; Blaschke, Daniel; Rebohle, Lars; Cherkouk, Charaf; Neugebauer, Ute; Schmidt, Oliver G.; Schmidt, Heidemarie. 2020. "Towards Bacteria Counting in DI Water of Several Microliters or Growing Suspension Using Impedance Biochips" Biosensors 10, no. 8: 82. https://doi.org/10.3390/bios10080082

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