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Open AccessArticle

Laccase-Based Biosensor Encapsulated in a Galactomannan-Chitosan Composite for the Evaluation of Phenolic Compounds

1
Laboratory of Applied Energetics and Materials, University of Jijel, Ouled Aissa 18000, Algeria
2
Institute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, France
*
Author to whom correspondence should be addressed.
Biosensors 2020, 10(6), 70; https://doi.org/10.3390/bios10060070
Received: 25 May 2020 / Revised: 11 June 2020 / Accepted: 19 June 2020 / Published: 22 June 2020
(This article belongs to the Special Issue Polymers-Based Biosensors)
Galactomannan, a neutral polysaccharide, was extracted from carob seeds and characterized. It was used for the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite. The fabricated biosensor was characterized by FTIR, scanning electron microscopy and cyclic voltammetry. The pyrocatechol detection was obtained by cyclic voltammetry measurements, through the detection of o-quinone at −0.447 V. The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10−16 M–10−4 M range was very high. The voltammetric response of the biosensor was stable for more than two weeks. To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin–Ciocalteu method. View Full-Text
Keywords: galactomannan; chitosan; laccase; voltammetry; phenolic content galactomannan; chitosan; laccase; voltammetry; phenolic content
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MDPI and ACS Style

Boubezari, I.; Bessueille, F.; Bonhomme, A.; Raimondi, G.; Zazoua, A.; Errachid, A.; Jaffrezic-Renault, N. Laccase-Based Biosensor Encapsulated in a Galactomannan-Chitosan Composite for the Evaluation of Phenolic Compounds. Biosensors 2020, 10, 70.

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