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Open AccessArticle

Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction

by Zui Tao 1,2,3, You Zhou 1,2,3, Xiang Li 1,2,3 and Zhouping Wang 1,2,3,4,*
1
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
2
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
3
International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China
4
Collaborative Innovation Center of Food safety and Quality Control of Jiangsu Province, Jiangnan University, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
Biosensors 2020, 10(4), 31; https://doi.org/10.3390/bios10040031
Received: 26 February 2020 / Revised: 19 March 2020 / Accepted: 25 March 2020 / Published: 30 March 2020
(This article belongs to the Special Issue Biomimetic Sensors and Their Applications)
Fumonisin B1 (FB1) is the most prevalent and toxic form among fumonisin homologues which are produced by fusarium species and it contaminates various types of food products, posing serious health hazards for humans and animals. In this work, a colorimetric assay for the detection of FB1 has been developed based on competitive horseradish peroxidase (HRP)-linked aptamer and dual biotin-streptavidin interaction. In short, a biotinylated aptamer of FB1 was immobilized on the microplate by biotin-streptavidin binding; the complementary strand (csDNA) of the aptamer was ligated with HRP by biotin-streptavidin binding again to form a csDNA-HRP sensing probe, competing with FB1 to bind to the aptamer. The color change can be observed after the addition of chromogenic and stop solution, thereby realizing the visual detection of FB1. Under optimal conditions, good linearity was observed within the concentration range of 0.5 to 300 ng/mL, with a detection of limit of 0.3 ng/mL. This assay is further validated by spike recovery tests towards beer and corn samples, it provides a simple, sensitive and reliable method for the screening of FB1 in food samples and may be potentially used as an alternative to conventional assays. View Full-Text
Keywords: aptamer; fumonisin B1; mycotoxin; horseradish peroxidase; food safety aptamer; fumonisin B1; mycotoxin; horseradish peroxidase; food safety
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MDPI and ACS Style

Tao, Z.; Zhou, Y.; Li, X.; Wang, Z. Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction. Biosensors 2020, 10, 31.

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