Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Apparatus
2.2. Preparation of the Complementary Strand-HRP Sensing Probe
2.3. Preparation of a Streptavidin-Coated Microplate
2.4. Detection Procedure
2.5. Preparation and Measurement of Food Samples
3. Results and Discussion
3.1. Principle of the Detection of FB1 by the Aptasensor
3.2. Optimization of Test Conditions
3.3. Detection Performances
3.4. Analysis for the Specificity
3.5. Analysis for the Stability
3.6. Determination of FB1 in Spiked Food Samples
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Detection Method | LOD (ng/mL) | Linear Range (ng/mL) | Reference |
---|---|---|---|
SPE based Electrochemical Immunosensor | 4.2 | 200–4500 | [27] |
Chemiluminescence ELISA | 0.35 | 0.93–7.73 | [28] |
Microarray-Based Immunoassay | 11.1 | 17.3–79.6 | [29] |
AuNPs based dc-pELISA | 3.07 | 3.125–25 | [41] |
Nanotubes based Electrochemical sensor | 0.002 | 0.01–1000 | [42] |
Colloidal gold immunoassay | 2.5 | 2.5–10 | [43] |
Aptamer based colorimetric assay | 0.3 | 0.5–300 | this work |
Number | Background (ng/mL) | Added (ng/mL) | Found (ELISA) (ng/mL) | Spike Recovery (ELISA) (%) | Found (ng/mL) | Spike Recovery (%) | |
---|---|---|---|---|---|---|---|
beer | 1 | 0 | 10 | 9.140 | 91.40 | 9.010 | 90.10 |
2 | 0 | 50 | 50.68 | 101.4 | 51.08 | 102.2 | |
3 | 0 | 200 | 197.4 | 98.69 | 197.2 | 98.60 |
Number | Background (µg/kg) | Added (µg/kg) | Found (ELISA) (µg/kg) | Spike Recovery (ELISA) (%) | Found (µg/kg) | Spike Recovery (%) | |
---|---|---|---|---|---|---|---|
corn | 1 | 0 | 10 | 9.260 | 92.60 | 9.180 | 91.80 |
2 | 0 | 50 | 52.21 | 104.4 | 52.60 | 105.2 | |
3 | 0 | 200 | 206.3 | 103.1 | 207.3 | 103.7 |
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Tao, Z.; Zhou, Y.; Li, X.; Wang, Z. Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction. Biosensors 2020, 10, 31. https://doi.org/10.3390/bios10040031
Tao Z, Zhou Y, Li X, Wang Z. Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction. Biosensors. 2020; 10(4):31. https://doi.org/10.3390/bios10040031
Chicago/Turabian StyleTao, Zui, You Zhou, Xiang Li, and Zhouping Wang. 2020. "Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction" Biosensors 10, no. 4: 31. https://doi.org/10.3390/bios10040031