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Open AccessArticle

Recyclable SERS-Based Immunoassay Guided by Photocatalytic Performance of Fe3O4@TiO2@Au Nanocomposites

Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, China
Institute of Solid State Physics, Shanxi Datong University, Datong 037009, China
XLIM Research Institute, UMR 7252 CNRS/University of Limoges, Avenue Albert Thomas, 87060 Limoges, France
Author to whom correspondence should be addressed.
Biosensors 2020, 10(3), 25;
Received: 14 January 2020 / Revised: 20 February 2020 / Accepted: 13 March 2020 / Published: 16 March 2020
A novel recyclable surface-enhanced Raman scattering (SERS)-based immunoassay was demonstrated and exhibited extremely high sensitivity toward prostate specific antigen (PSA). The immunoassay, which possessed a sandwich structure, was constructed of multifunctional Fe3O4@TiO2@Au nanocomposites as immune probe and Ag-coated sandpaper as immune substrate. First, by adjusting the density of outside Au seeds on Fe3O4@TiO2 core-shell nanoparticles (NPs), the structure-dependent SERS and photocatalytic performance of the samples was explored by monitoring and degradating 4-mercaptobenzonic acid (4MBA). Afterwards, the SERS enhancement capability of Ag-coated sandpaper with different meshes was investigated, and a limit of detection (LOD), as low as 0.014 mM, was achieved by utilizing the substrate. Subsequently, the recyclable feasibility of PSA detection was approved by zeta potential measurement, absorption spectra, and SEM images and, particularly, more than 80% of SERS intensity still existed after even six cycles of immunoassay. The ultralow LOD of the recyclable immunoassay was finally calculated to be 1.871 pg/mL. Therefore, the recyclable SERS-based immunoassay exhibits good application prospects for diagnosis of cancer in clinical measurements. View Full-Text
Keywords: recyclable immunoassay; PSA; Fe3O4@TiO2@Au; Ag-coated sandpaper recyclable immunoassay; PSA; Fe3O4@TiO2@Au; Ag-coated sandpaper
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Du, Y.; Liu, H.; Tian, Y.; Gu, C.; Zhao, Z.; Zeng, S.; Jiang, T. Recyclable SERS-Based Immunoassay Guided by Photocatalytic Performance of Fe3O4@TiO2@Au Nanocomposites. Biosensors 2020, 10, 25.

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